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The map label for this gene is metJ [H]

Identifier: 218710812

GI number: 218710812

Start: 3048733

End: 3049053

Strand: Direct

Name: metJ [H]

Synonym: VS_2895

Alternate gene names: 218710812

Gene position: 3048733-3049053 (Clockwise)

Preceding gene: 218710808

Following gene: 218710815

Centisome position: 92.41

GC content: 45.79

Gene sequence:

>321_bases
ATGGCCGACTGGAATGGTGAATACATAAGCCCATATGCTGAGCATGGAAAGAAAAGCGAACAAGTAAAGAAAATTACAGT
TTCTATCCCTCTAAAAGTGTTAAAGGTTCTTACTGACGAGCGCACTCGCCGCCAGATCAATAACCTACGCCATGCAACAA
ACAGTGAGCTACTGTGCGAAGCCTTTCTACATGCGTACACAGGCCAACCACTGCCAACGGATGAAGACCTTCGTAAAGAT
CGTCCAGACGACATCCCGACTGAAGTCAAAAAACTGATGACAGAGATGGGCATCGAGTTCGAAGCGTTTGACGAAGAATA
A

Upstream 100 bases:

>100_bases
AACACTTCCGGACGTCTATATGTCTTTGCTTATGGCAGTAAATCCCGCTAAAATCAGCACTATTAATTTATTCTAACAAC
TAAAGTGATGAAGGTACGCA

Downstream 100 bases:

>100_bases
AAACAACTCAGTCACCGCCTTCATTATTGATTCGCTATAGCTGAACCCTATTTGTCACATCACCCACTACGTTATCTGTA
GCTGCGCTGTGAATGATAGA

Product: transcriptional repressor protein MetJ

Products: NA

Alternate protein names: Met regulon regulatory protein metJ [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MADWNGEYISPYAEHGKKSEQVKKITVSIPLKVLKVLTDERTRRQINNLRHATNSELLCEAFLHAYTGQPLPTDEDLRKD
RPDDIPTEVKKLMTEMGIEFEAFDEE

Sequences:

>Translated_106_residues
MADWNGEYISPYAEHGKKSEQVKKITVSIPLKVLKVLTDERTRRQINNLRHATNSELLCEAFLHAYTGQPLPTDEDLRKD
RPDDIPTEVKKLMTEMGIEFEAFDEE
>Mature_105_residues
ADWNGEYISPYAEHGKKSEQVKKITVSIPLKVLKVLTDERTRRQINNLRHATNSELLCEAFLHAYTGQPLPTDEDLRKDR
PDDIPTEVKKLMTEMGIEFEAFDEE

Specific function: This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis [H]

COG id: COG3060

COG function: function code KE; Transcriptional regulator of met regulon

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metJ family [H]

Homologues:

Organism=Escherichia coli, GI1790373, Length=104, Percent_Identity=79.8076923076923, Blast_Score=179, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002084
- InterPro:   IPR010985
- ProDom:   PD020365 [H]

Pfam domain/function: PF01340 MetJ [H]

EC number: NA

Molecular weight: Translated: 12275; Mature: 12144

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWNGEYISPYAEHGKKSEQVKKITVSIPLKVLKVLTDERTRRQINNLRHATNSELLCE
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAYTGQPLPTDEDLRKDRPDDIPTEVKKLMTEMGIEFEAFDEE
HHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADWNGEYISPYAEHGKKSEQVKKITVSIPLKVLKVLTDERTRRQINNLRHATNSELLCE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAYTGQPLPTDEDLRKDRPDDIPTEVKKLMTEMGIEFEAFDEE
HHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA