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The map label for this gene is priA [H]

Identifier: 218710815

GI number: 218710815

Start: 3051424

End: 3053625

Strand: Direct

Name: priA [H]

Synonym: VS_2898

Alternate gene names: 218710815

Gene position: 3051424-3053625 (Clockwise)

Preceding gene: 218710812

Following gene: 218710816

Centisome position: 92.49

GC content: 48.73

Gene sequence:

>2202_bases
ATGCGTCCAATGATTGCCCGTGTGGCACTGCCTGTTCCACTCGACAAGCAGTTCGATTACAAGATTCCTAGCCACCTATT
TCCGATTATTGGCGGTCGAGTCTCTGTGCCTTTTGGACGACAAACCTTAACAGGCATTGTTACGGCTCTGGTTAACGAAT
CTGAATTCGAGTTAGACAAACTCAAGCCCATTAAAGCCTTACTAGACAACCAACCCGTTTGGCCGGAATCGGTCTACTCA
CTCTTGGTATGGTGCAGCCAGTTCTATCAGTATCCACTAGGTGAAACCTTGGCCAACGCACTACCAAGCGCCTTACGAAA
GGGCAAAGCGGCCGATTTCGCGACGCTTGTTGAATGGCAACTGACCTCGTCAGGTAGAGATCAACTGATGCAGGGCTTTG
GCCGTGCCGTCAAACAAGCCAAAGTAATGCACATGCTTGAGCACGGCCCAGTTCCCCATCAAGAATTCATTGATGAAGAA
GTGGGCAGCGCGGTACTTAAGACGTTGGAAGAGAAAGGCTGGGTTGAATCAGTAGAGAAAAAGCCGAAGCGCCAGCCGTG
GCCAGTTGAGCTCGAAAACGATCAAGATAAGCCAAAACTCAATGAAGAACAGGCTATAGCAATAGCGACAGTAAATAGTC
AAACCGACTTTGGGTGCTTCTTGCTAGAGGGTGTAACTGGCTCAGGTAAGACCGAAGTCTACCTGAATATGATCAAGCCG
ATTCTCGACCAAGGTAAACAAGCGTTAGTGTTAGTGCCCGAGATCGGTCTAACCCCTCAAACGATTAACCGCTTTAAGCG
ACGCTTTAATGTTCCAGTTGAGGTTATCCATTCTGGTTTAAACGATTCTGAGCGACTAAATGCTTGGTTGTCGGCGCGTG
ATAAAATAGCGGGCATTGTGATCGGTACTCGTTCAGCTCTGTTCACACCGTTCGCGGATCTGGGCATTATTATTGTCGAT
GAAGAGCACGACGCCTCTTATAAGCAGCAAGATAGCCTACGCTACCACGCTCGTGATGTTGCCATCATGCGTGCTCATAA
AGCGCAGATTCCGGTTGTGCTAGGCTCGGCGACTCCGGCCTTTGAAACGCTGCACAACGCTCAGATCGGTAAATACAGTT
ACCTCACCCTGACCTCACGCGCAGGTGTCGCGCTGCCGACCACCAATAAAGTGTTGGACGTTAAGGGGGAATATCTAGAA
AGTGGCTTGTCTGCGTCGTTGATTGCTGAAATGCAAAGACACCTCAAAGCAGGCAACCAAGTGATGTTGTTCCTCAATCG
TCGTGGGTTTTCTCCCGCATTAATGTGTCACGATTGTGGTTGGACAGCAGAATGTAAGCGTTGTGATGCCTACTACACCT
ATCATCAATACAGCAATGAGATGCGCTGCCACCACTGTGGCTCGCAGCAGCATATCGTGCATCATTGCCAAGGTTGTGGT
TCAGCGAATTTAGTAACAGTGGGGGTTGGTACCGAACAGTTAGAGGCTCAACTGGGTCAACTATTCCCTGAATACAAAAC
CATCCGTATTGACCGAGACAGCACTCGCCGCAAAGGCAGTTTAGAAAGCGCGTTAGAATCGATTCGTAAAGGTGAATACC
AAATTCTGATTGGTACACAGATGCTTGCTAAAGGGCATCACTTCCCAGATGTGACACTTGTGGCATTGCTGGACGTTGAT
GCCTCTTTATATAGCAGTGACTTCCGCGCCTCTGAGCGATTGGCTCAATTGTTTACTCAAGTCGCCGGTCGAGCAGGACG
AGCCAGTAAGCCGGGGGAAGTGATCTTACAAACTCACCATCCAGAACACGGCTTATTACAGGCATTACTGCACAAAAACT
ACAACCACTTTGCTCAGACTGCATTAGCAGAGCGTAAACAAGCGATGTTGCCGCCTTATACCTTCATGACTCTGTTTAGA
GCGGAAGCCAATGACACTCGCTTAGTGGAAGAGTTTTTGCGTCAAGTGCGCCACACATTGGAATCGCATCCGTTATTTGA
CCAATATTGTATGGTGCTAGGGCCAACCCCTGCACCACTAGCCAAACGAGCAGGTAAATCTCGCTGGCAGCTTATTTTAC
AAACACAGACTCGCTCGTTGATGCAAAAGCTATTAATGAGCGCCAAGCCAGCAATCAATATGTTACCCGCTGCGAAAAAA
GTCCGTTGGTCACTCGATATTGAGCCGCAAGATCTGAGCTAA

Upstream 100 bases:

>100_bases
AAGCCTCAAGTAAGCTCTGTCATCTAAACTGCTCCTCGAATTTAAGGCCCTTATCCATTAGACTGTTCGCTCTCGTCAAC
TCAGATAAAGCCGTCCCTTT

Downstream 100 bases:

>100_bases
ACCCACTTGGGTTAAAACTAATGATAAGTTTGTTCACAAATATTTCATATTTCTCGTGAGCAACTTCACACTAGTCATGT
GATTTTTGTTAATCTAGCCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MRPMIARVALPVPLDKQFDYKIPSHLFPIIGGRVSVPFGRQTLTGIVTALVNESEFELDKLKPIKALLDNQPVWPESVYS
LLVWCSQFYQYPLGETLANALPSALRKGKAADFATLVEWQLTSSGRDQLMQGFGRAVKQAKVMHMLEHGPVPHQEFIDEE
VGSAVLKTLEEKGWVESVEKKPKRQPWPVELENDQDKPKLNEEQAIAIATVNSQTDFGCFLLEGVTGSGKTEVYLNMIKP
ILDQGKQALVLVPEIGLTPQTINRFKRRFNVPVEVIHSGLNDSERLNAWLSARDKIAGIVIGTRSALFTPFADLGIIIVD
EEHDASYKQQDSLRYHARDVAIMRAHKAQIPVVLGSATPAFETLHNAQIGKYSYLTLTSRAGVALPTTNKVLDVKGEYLE
SGLSASLIAEMQRHLKAGNQVMLFLNRRGFSPALMCHDCGWTAECKRCDAYYTYHQYSNEMRCHHCGSQQHIVHHCQGCG
SANLVTVGVGTEQLEAQLGQLFPEYKTIRIDRDSTRRKGSLESALESIRKGEYQILIGTQMLAKGHHFPDVTLVALLDVD
ASLYSSDFRASERLAQLFTQVAGRAGRASKPGEVILQTHHPEHGLLQALLHKNYNHFAQTALAERKQAMLPPYTFMTLFR
AEANDTRLVEEFLRQVRHTLESHPLFDQYCMVLGPTPAPLAKRAGKSRWQLILQTQTRSLMQKLLMSAKPAINMLPAAKK
VRWSLDIEPQDLS

Sequences:

>Translated_733_residues
MRPMIARVALPVPLDKQFDYKIPSHLFPIIGGRVSVPFGRQTLTGIVTALVNESEFELDKLKPIKALLDNQPVWPESVYS
LLVWCSQFYQYPLGETLANALPSALRKGKAADFATLVEWQLTSSGRDQLMQGFGRAVKQAKVMHMLEHGPVPHQEFIDEE
VGSAVLKTLEEKGWVESVEKKPKRQPWPVELENDQDKPKLNEEQAIAIATVNSQTDFGCFLLEGVTGSGKTEVYLNMIKP
ILDQGKQALVLVPEIGLTPQTINRFKRRFNVPVEVIHSGLNDSERLNAWLSARDKIAGIVIGTRSALFTPFADLGIIIVD
EEHDASYKQQDSLRYHARDVAIMRAHKAQIPVVLGSATPAFETLHNAQIGKYSYLTLTSRAGVALPTTNKVLDVKGEYLE
SGLSASLIAEMQRHLKAGNQVMLFLNRRGFSPALMCHDCGWTAECKRCDAYYTYHQYSNEMRCHHCGSQQHIVHHCQGCG
SANLVTVGVGTEQLEAQLGQLFPEYKTIRIDRDSTRRKGSLESALESIRKGEYQILIGTQMLAKGHHFPDVTLVALLDVD
ASLYSSDFRASERLAQLFTQVAGRAGRASKPGEVILQTHHPEHGLLQALLHKNYNHFAQTALAERKQAMLPPYTFMTLFR
AEANDTRLVEEFLRQVRHTLESHPLFDQYCMVLGPTPAPLAKRAGKSRWQLILQTQTRSLMQKLLMSAKPAINMLPAAKK
VRWSLDIEPQDLS
>Mature_733_residues
MRPMIARVALPVPLDKQFDYKIPSHLFPIIGGRVSVPFGRQTLTGIVTALVNESEFELDKLKPIKALLDNQPVWPESVYS
LLVWCSQFYQYPLGETLANALPSALRKGKAADFATLVEWQLTSSGRDQLMQGFGRAVKQAKVMHMLEHGPVPHQEFIDEE
VGSAVLKTLEEKGWVESVEKKPKRQPWPVELENDQDKPKLNEEQAIAIATVNSQTDFGCFLLEGVTGSGKTEVYLNMIKP
ILDQGKQALVLVPEIGLTPQTINRFKRRFNVPVEVIHSGLNDSERLNAWLSARDKIAGIVIGTRSALFTPFADLGIIIVD
EEHDASYKQQDSLRYHARDVAIMRAHKAQIPVVLGSATPAFETLHNAQIGKYSYLTLTSRAGVALPTTNKVLDVKGEYLE
SGLSASLIAEMQRHLKAGNQVMLFLNRRGFSPALMCHDCGWTAECKRCDAYYTYHQYSNEMRCHHCGSQQHIVHHCQGCG
SANLVTVGVGTEQLEAQLGQLFPEYKTIRIDRDSTRRKGSLESALESIRKGEYQILIGTQMLAKGHHFPDVTLVALLDVD
ASLYSSDFRASERLAQLFTQVAGRAGRASKPGEVILQTHHPEHGLLQALLHKNYNHFAQTALAERKQAMLPPYTFMTLFR
AEANDTRLVEEFLRQVRHTLESHPLFDQYCMVLGPTPAPLAKRAGKSRWQLILQTQTRSLMQKLLMSAKPAINMLPAAKK
VRWSLDIEPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=727, Percent_Identity=53.0949105914718, Blast_Score=791, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82063; Mature: 82063

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPMIARVALPVPLDKQFDYKIPSHLFPIIGGRVSVPFGRQTLTGIVTALVNESEFELDK
CCCCHHHEECCCCCCCCCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHH
LKPIKALLDNQPVWPESVYSLLVWCSQFYQYPLGETLANALPSALRKGKAADFATLVEWQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHEEEE
LTSSGRDQLMQGFGRAVKQAKVMHMLEHGPVPHQEFIDEEVGSAVLKTLEEKGWVESVEK
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KPKRQPWPVELENDQDKPKLNEEQAIAIATVNSQTDFGCFLLEGVTGSGKTEVYLNMIKP
CCCCCCCCEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHH
ILDQGKQALVLVPEIGLTPQTINRFKRRFNVPVEVIHSGLNDSERLNAWLSARDKIAGIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCEEEEE
IGTRSALFTPFADLGIIIVDEEHDASYKQQDSLRYHARDVAIMRAHKAQIPVVLGSATPA
EECCHHHHCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHH
FETLHNAQIGKYSYLTLTSRAGVALPTTNKVLDVKGEYLESGLSASLIAEMQRHLKAGNQ
HHHHHCCCCCCEEEEEEECCCCEECCCCCCEEECCHHHHHCCCCHHHHHHHHHHHCCCCE
VMLFLNRRGFSPALMCHDCGWTAECKRCDAYYTYHQYSNEMRCHHCGSQQHIVHHCQGCG
EEEEEECCCCCCEEEEECCCCCHHHHHCCHHHHHHHHCCCCHHHHCCCHHHHHHHHCCCC
SANLVTVGVGTEQLEAQLGQLFPEYKTIRIDRDSTRRKGSLESALESIRKGEYQILIGTQ
CCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEHH
MLAKGHHFPDVTLVALLDVDASLYSSDFRASERLAQLFTQVAGRAGRASKPGEVILQTHH
HHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
PEHGLLQALLHKNYNHFAQTALAERKQAMLPPYTFMTLFRAEANDTRLVEEFLRQVRHTL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ESHPLFDQYCMVLGPTPAPLAKRAGKSRWQLILQTQTRSLMQKLLMSAKPAINMLPAAKK
HCCCCHHHHHHHCCCCCCHHHHHCCCCHHHEEHHHHHHHHHHHHHHHCCCCHHHCCCCCC
VRWSLDIEPQDLS
EEEEECCCCCCCC
>Mature Secondary Structure
MRPMIARVALPVPLDKQFDYKIPSHLFPIIGGRVSVPFGRQTLTGIVTALVNESEFELDK
CCCCHHHEECCCCCCCCCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHH
LKPIKALLDNQPVWPESVYSLLVWCSQFYQYPLGETLANALPSALRKGKAADFATLVEWQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHEEEE
LTSSGRDQLMQGFGRAVKQAKVMHMLEHGPVPHQEFIDEEVGSAVLKTLEEKGWVESVEK
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KPKRQPWPVELENDQDKPKLNEEQAIAIATVNSQTDFGCFLLEGVTGSGKTEVYLNMIKP
CCCCCCCCEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHH
ILDQGKQALVLVPEIGLTPQTINRFKRRFNVPVEVIHSGLNDSERLNAWLSARDKIAGIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCEEEEE
IGTRSALFTPFADLGIIIVDEEHDASYKQQDSLRYHARDVAIMRAHKAQIPVVLGSATPA
EECCHHHHCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHH
FETLHNAQIGKYSYLTLTSRAGVALPTTNKVLDVKGEYLESGLSASLIAEMQRHLKAGNQ
HHHHHCCCCCCEEEEEEECCCCEECCCCCCEEECCHHHHHCCCCHHHHHHHHHHHCCCCE
VMLFLNRRGFSPALMCHDCGWTAECKRCDAYYTYHQYSNEMRCHHCGSQQHIVHHCQGCG
EEEEEECCCCCCEEEEECCCCCHHHHHCCHHHHHHHHCCCCHHHHCCCHHHHHHHHCCCC
SANLVTVGVGTEQLEAQLGQLFPEYKTIRIDRDSTRRKGSLESALESIRKGEYQILIGTQ
CCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEHH
MLAKGHHFPDVTLVALLDVDASLYSSDFRASERLAQLFTQVAGRAGRASKPGEVILQTHH
HHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
PEHGLLQALLHKNYNHFAQTALAERKQAMLPPYTFMTLFRAEANDTRLVEEFLRQVRHTL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ESHPLFDQYCMVLGPTPAPLAKRAGKSRWQLILQTQTRSLMQKLLMSAKPAINMLPAAKK
HCCCCHHHHHHHCCCCCCHHHHHCCCCHHHEEHHHHHHHHHHHHHHHCCCCHHHCCCCCC
VRWSLDIEPQDLS
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]