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The map label for this gene is metL [H]

Identifier: 218710810

GI number: 218710810

Start: 3044929

End: 3047340

Strand: Reverse

Name: metL [H]

Synonym: VS_2893

Alternate gene names: 218710810

Gene position: 3047340-3044929 (Counterclockwise)

Preceding gene: 218710811

Following gene: 218710809

Centisome position: 92.36

GC content: 49.0

Gene sequence:

>2412_bases
ATGACAACCTTTCGCCAGCTACATAAATTTGGTGGCAGCAGTTTAGCGAACCCAGAGTGTTACCAACGCGTGGTCAATAT
TCTTAGAGAATACTCATCAACTACTGACTTGGTCGTGGTATCAGCTGCGGGTAAAACAACTAACCGCTTGATTGAGTTTG
TTGAAGCGCTCGACAAAGATGGTCGTATCGCTCACGAATGTCTACAAACCCTTCGTCAGTTCCAACTTGAGCTGATTGAA
GCGCTGCTTGAAGGTGAGTCTGCGGCTCAGCTAACGGCCGCTATTCAACAAGAATTTACTGCTTTGGGTGAGCTAACTGC
TCCTCTAAGCGAATCGCAAAAAGCACAGGTGTTAGGACACGGTGAGGTTTGGTCTTCACGTTTGTTAGCCGCTCTGTTGT
GCCAACATGACTTACAAGCAGTTGCTCAAGATGCGCGTGCCTTTTTACGTGCAGAAGCGGGTGCTCAACCCGAAGTGGAT
CGCGCGCGTTCTTATCCTCTGATTAAAGAAGTTTTGGCTCAGCATACGCATTGCCGAGTGGTGATTACGGGCTTTATGGC
TCAGAACTGCGAAGGCGAAACGGTACTGCTAGGTCGTAACGGCTCGGATTACTCAGCAACTGTGATTGGCGCCTTGGCTG
AAGTTGAACGTGTGACGATTTGGAGTGACGTGGCGGGCGTATACAGCGCTGACCCTCGTTTGGTTTCGGATGCGTGTTTA
CTGCCTCTGCTTCGCCTTGATGAAGCGAGTGAACTCGCTCGTCTGGCGGCTCCAGTGCTCCATAGCCGAACGCTACAGCC
TGTGGCTCAAAGTGCTATGGATCTCAGCTTGCGCTGCAGCTACCAGCCGGAGGCGGGCTCTACACAGATAGAGCGTGTAC
TGGCATCGGGTCGTGGCGCAAAAATTATTACCTCTCTGGATGAAGTTCTGATCGTGCAGCTGACCTTTGGTCATGGCCAT
GATTTTGACCGTCTAGAAAGTGAAGTACTGGAAGGGCTTAAGCGAGCTCAATTAGAGCCGCTTGCTTATGAGCTTGAACC
GGATCAGCACTGCTTACGTCTTGCTTATACAGAAGAGATCGCTGGTGGTGCATTAGAATATCTACAAGACCACGCGATTG
AAGCGGAGATTAAGCTCAAAGAAGGTTTCTCTCTTATCGCGGCGGTAGGTGCGGGTGTGACTAAGAACCCGAACCATTGC
TACGGTTTCTACCAGCAGCTCAAGAGCTCGCCAGTTGAGTTTATCTCAGAAGCGAACTCTGGCTTGAGCTTAGTCGCTGT
GATTCGTAAGAGTGAGACATCAAGCCTAGTGAAAGGCATTCACTCTCAACTGTTCCAAGCGCAGAAGCGTGTTGCGATTG
CCTTGTGTGGCAAGGGCAATATTGGTTCAAGTTGGTTAAGCCTGTTTGCTGAGCAAAAGGCGGAACTTGAAAAGCGCCGT
GGGATGAACTTTGAATTGGTTGCGGTGGTTGATAGCCAAACTTATTGGTTCGACGATCAAGGCATTGATGCGACTTCAGT
TGGTAAACGCTTTGATGACGAAGCGATTGCCAATAATGGTAATGACTGGTTAGAGCGTTTGGGTTCTATTCAGGGTTACG
ATGAAGCTGTAGTACTCGATGTCACTGCAAGCCCAGTGCTTGCAGCAAAATATCTGCAAGTCGCACAACAAGGTATTCAC
CTGATTTCGGCTAACAAGGTGGCTGGTTCTGCATCGAGCGAGTATTACCATCAAGTACAGGATGCTTTCGCTAAGATCAG
CCGTCACTGGTTGTACAACGCGACCGTTGGTGCAGGCCTGCCGATTAACCACACAGTCAGAGACTTGCGTGAAAGTGGCG
ATGACATTATTGCGCTGTCAGGTATCTTTTCAGGCACGCTGTCTTGGTTGTTCCAACAGTTTGATGGCACCGTGCCATTC
AGCGAGTTGGTTGACTTGGCATGGCAACAAGGTCTTACGGAACCAGACCCTCGTGCTGACCTAGATGGTTCAGACGTGAT
GCGTAAGCTGGTGATTCTGGCGCGTGAATCGGGTTTAGATATTGAGCCTGAAAACGTCAAAGTAGAATCATTGGTACCTG
AAGAATTACAAGATCTGTCAGTGGATGACTTCTTTGATAAAGCTTCTGTACTGAGTGAAGAGTTGGCCGAGCGTTTAGAG
AAAGCGCAGTCTCAACAGAAGGTCCTTCGTTACGTAGCGCGTTTAGAGAAGAGTGGTAAGGCAACGGTAGGCGTTGAAGC
GTTATCTAAAGAACACGCTCTGGCGAACTTACTGCCTTGCGACAACATCTTTGCGATTGAGAGCAAATGGTACAAAGATA
ATCCATTGGTTATTCGTGGCCCGGGTGCTGGTCGTGAAGTAACAGCTGGTGCAATTCAGTCTGATTTAAACAGAATGTCT
AGCCTGTTTTAA

Upstream 100 bases:

>100_bases
GCCTTTCTGTTGGCCTTGAAGATGCAGAAGACTTGATTGATGATCTCAAGCAAGCGTTTGAAAAAACACAAGGCTTTATC
GCAGAAGGGGAGGTTTAATA

Downstream 100 bases:

>100_bases
TACTGAGAGCGTTTGGCTTAATACTGAGAGCGTTTGGCTTAATACTTAGAGCGATTGGCATAATAGTCAGATCAATTGCT
TTAATACTCAGATTAATTGT

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MTTFRQLHKFGGSSLANPECYQRVVNILREYSSTTDLVVVSAAGKTTNRLIEFVEALDKDGRIAHECLQTLRQFQLELIE
ALLEGESAAQLTAAIQQEFTALGELTAPLSESQKAQVLGHGEVWSSRLLAALLCQHDLQAVAQDARAFLRAEAGAQPEVD
RARSYPLIKEVLAQHTHCRVVITGFMAQNCEGETVLLGRNGSDYSATVIGALAEVERVTIWSDVAGVYSADPRLVSDACL
LPLLRLDEASELARLAAPVLHSRTLQPVAQSAMDLSLRCSYQPEAGSTQIERVLASGRGAKIITSLDEVLIVQLTFGHGH
DFDRLESEVLEGLKRAQLEPLAYELEPDQHCLRLAYTEEIAGGALEYLQDHAIEAEIKLKEGFSLIAAVGAGVTKNPNHC
YGFYQQLKSSPVEFISEANSGLSLVAVIRKSETSSLVKGIHSQLFQAQKRVAIALCGKGNIGSSWLSLFAEQKAELEKRR
GMNFELVAVVDSQTYWFDDQGIDATSVGKRFDDEAIANNGNDWLERLGSIQGYDEAVVLDVTASPVLAAKYLQVAQQGIH
LISANKVAGSASSEYYHQVQDAFAKISRHWLYNATVGAGLPINHTVRDLRESGDDIIALSGIFSGTLSWLFQQFDGTVPF
SELVDLAWQQGLTEPDPRADLDGSDVMRKLVILARESGLDIEPENVKVESLVPEELQDLSVDDFFDKASVLSEELAERLE
KAQSQQKVLRYVARLEKSGKATVGVEALSKEHALANLLPCDNIFAIESKWYKDNPLVIRGPGAGREVTAGAIQSDLNRMS
SLF

Sequences:

>Translated_803_residues
MTTFRQLHKFGGSSLANPECYQRVVNILREYSSTTDLVVVSAAGKTTNRLIEFVEALDKDGRIAHECLQTLRQFQLELIE
ALLEGESAAQLTAAIQQEFTALGELTAPLSESQKAQVLGHGEVWSSRLLAALLCQHDLQAVAQDARAFLRAEAGAQPEVD
RARSYPLIKEVLAQHTHCRVVITGFMAQNCEGETVLLGRNGSDYSATVIGALAEVERVTIWSDVAGVYSADPRLVSDACL
LPLLRLDEASELARLAAPVLHSRTLQPVAQSAMDLSLRCSYQPEAGSTQIERVLASGRGAKIITSLDEVLIVQLTFGHGH
DFDRLESEVLEGLKRAQLEPLAYELEPDQHCLRLAYTEEIAGGALEYLQDHAIEAEIKLKEGFSLIAAVGAGVTKNPNHC
YGFYQQLKSSPVEFISEANSGLSLVAVIRKSETSSLVKGIHSQLFQAQKRVAIALCGKGNIGSSWLSLFAEQKAELEKRR
GMNFELVAVVDSQTYWFDDQGIDATSVGKRFDDEAIANNGNDWLERLGSIQGYDEAVVLDVTASPVLAAKYLQVAQQGIH
LISANKVAGSASSEYYHQVQDAFAKISRHWLYNATVGAGLPINHTVRDLRESGDDIIALSGIFSGTLSWLFQQFDGTVPF
SELVDLAWQQGLTEPDPRADLDGSDVMRKLVILARESGLDIEPENVKVESLVPEELQDLSVDDFFDKASVLSEELAERLE
KAQSQQKVLRYVARLEKSGKATVGVEALSKEHALANLLPCDNIFAIESKWYKDNPLVIRGPGAGREVTAGAIQSDLNRMS
SLF
>Mature_802_residues
TTFRQLHKFGGSSLANPECYQRVVNILREYSSTTDLVVVSAAGKTTNRLIEFVEALDKDGRIAHECLQTLRQFQLELIEA
LLEGESAAQLTAAIQQEFTALGELTAPLSESQKAQVLGHGEVWSSRLLAALLCQHDLQAVAQDARAFLRAEAGAQPEVDR
ARSYPLIKEVLAQHTHCRVVITGFMAQNCEGETVLLGRNGSDYSATVIGALAEVERVTIWSDVAGVYSADPRLVSDACLL
PLLRLDEASELARLAAPVLHSRTLQPVAQSAMDLSLRCSYQPEAGSTQIERVLASGRGAKIITSLDEVLIVQLTFGHGHD
FDRLESEVLEGLKRAQLEPLAYELEPDQHCLRLAYTEEIAGGALEYLQDHAIEAEIKLKEGFSLIAAVGAGVTKNPNHCY
GFYQQLKSSPVEFISEANSGLSLVAVIRKSETSSLVKGIHSQLFQAQKRVAIALCGKGNIGSSWLSLFAEQKAELEKRRG
MNFELVAVVDSQTYWFDDQGIDATSVGKRFDDEAIANNGNDWLERLGSIQGYDEAVVLDVTASPVLAAKYLQVAQQGIHL
ISANKVAGSASSEYYHQVQDAFAKISRHWLYNATVGAGLPINHTVRDLRESGDDIIALSGIFSGTLSWLFQQFDGTVPFS
ELVDLAWQQGLTEPDPRADLDGSDVMRKLVILARESGLDIEPENVKVESLVPEELQDLSVDDFFDKASVLSEELAERLEK
AQSQQKVLRYVARLEKSGKATVGVEALSKEHALANLLPCDNIFAIESKWYKDNPLVIRGPGAGREVTAGAIQSDLNRMSS
LF

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=803, Percent_Identity=57.7833125778331, Blast_Score=934, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=840, Percent_Identity=29.6428571428571, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=26.8980477223427, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322599, Length=358, Percent_Identity=31.5642458100559, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320893, Length=366, Percent_Identity=21.5846994535519, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 87805; Mature: 87674

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFRQLHKFGGSSLANPECYQRVVNILREYSSTTDLVVVSAAGKTTNRLIEFVEALDKD
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCC
GRIAHECLQTLRQFQLELIEALLEGESAAQLTAAIQQEFTALGELTAPLSESQKAQVLGH
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEC
GEVWSSRLLAALLCQHDLQAVAQDARAFLRAEAGAQPEVDRARSYPLIKEVLAQHTHCRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCCCCEE
VITGFMAQNCEGETVLLGRNGSDYSATVIGALAEVERVTIWSDVAGVYSADPRLVSDACL
EEEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LPLLRLDEASELARLAAPVLHSRTLQPVAQSAMDLSLRCSYQPEAGSTQIERVLASGRGA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHCCCCC
KIITSLDEVLIVQLTFGHGHDFDRLESEVLEGLKRAQLEPLAYELEPDQHCLRLAYTEEI
HHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHH
AGGALEYLQDHAIEAEIKLKEGFSLIAAVGAGVTKNPNHCYGFYQQLKSSPVEFISEANS
CCHHHHHHHHHCCCEEEEHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCC
GLSLVAVIRKSETSSLVKGIHSQLFQAQKRVAIALCGKGNIGSSWLSLFAEQKAELEKRR
CCEEEEEHHHCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
GMNFELVAVVDSQTYWFDDQGIDATSVGKRFDDEAIANNGNDWLERLGSIQGYDEAVVLD
CCCEEEEEEEECCEEEECCCCCCHHHHCCCCCCHHHHCCCHHHHHHHCCCCCCCCEEEEE
VTASPVLAAKYLQVAQQGIHLISANKVAGSASSEYYHQVQDAFAKISRHWLYNATVGAGL
ECCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCC
PINHTVRDLRESGDDIIALSGIFSGTLSWLFQQFDGTVPFSELVDLAWQQGLTEPDPRAD
CCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
LDGSDVMRKLVILARESGLDIEPENVKVESLVPEELQDLSVDDFFDKASVLSEELAERLE
CCHHHHHHHHHHHHHCCCCCCCCCCEEEHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
KAQSQQKVLRYVARLEKSGKATVGVEALSKEHALANLLPCDNIFAIESKWYKDNPLVIRG
HHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEC
PGAGREVTAGAIQSDLNRMSSLF
CCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTFRQLHKFGGSSLANPECYQRVVNILREYSSTTDLVVVSAAGKTTNRLIEFVEALDKD
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCC
GRIAHECLQTLRQFQLELIEALLEGESAAQLTAAIQQEFTALGELTAPLSESQKAQVLGH
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEC
GEVWSSRLLAALLCQHDLQAVAQDARAFLRAEAGAQPEVDRARSYPLIKEVLAQHTHCRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHCCCCCEE
VITGFMAQNCEGETVLLGRNGSDYSATVIGALAEVERVTIWSDVAGVYSADPRLVSDACL
EEEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LPLLRLDEASELARLAAPVLHSRTLQPVAQSAMDLSLRCSYQPEAGSTQIERVLASGRGA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHCCCCC
KIITSLDEVLIVQLTFGHGHDFDRLESEVLEGLKRAQLEPLAYELEPDQHCLRLAYTEEI
HHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHH
AGGALEYLQDHAIEAEIKLKEGFSLIAAVGAGVTKNPNHCYGFYQQLKSSPVEFISEANS
CCHHHHHHHHHCCCEEEEHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCC
GLSLVAVIRKSETSSLVKGIHSQLFQAQKRVAIALCGKGNIGSSWLSLFAEQKAELEKRR
CCEEEEEHHHCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
GMNFELVAVVDSQTYWFDDQGIDATSVGKRFDDEAIANNGNDWLERLGSIQGYDEAVVLD
CCCEEEEEEEECCEEEECCCCCCHHHHCCCCCCHHHHCCCHHHHHHHCCCCCCCCEEEEE
VTASPVLAAKYLQVAQQGIHLISANKVAGSASSEYYHQVQDAFAKISRHWLYNATVGAGL
ECCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCC
PINHTVRDLRESGDDIIALSGIFSGTLSWLFQQFDGTVPFSELVDLAWQQGLTEPDPRAD
CCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
LDGSDVMRKLVILARESGLDIEPENVKVESLVPEELQDLSVDDFFDKASVLSEELAERLE
CCHHHHHHHHHHHHHCCCCCCCCCCEEEHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
KAQSQQKVLRYVARLEKSGKATVGVEALSKEHALANLLPCDNIFAIESKWYKDNPLVIRG
HHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEC
PGAGREVTAGAIQSDLNRMSSLF
CCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]