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The map label for this gene is metF [H]

Identifier: 218710809

GI number: 218710809

Start: 3043669

End: 3044682

Strand: Reverse

Name: metF [H]

Synonym: VS_2892

Alternate gene names: 218710809

Gene position: 3044682-3043669 (Counterclockwise)

Preceding gene: 218710810

Following gene: 218710805

Centisome position: 92.28

GC content: 44.87

Gene sequence:

>1014_bases
ATGGATCTGTTGACATTAAATCGTATTCAATTCATTCTGGAGACATATAGACGTCTAAACGTCGCTAGGGATTTGAGATT
ATTTAGTGGTTGCTTTTGCCACAGGGAGAGTAAGATGGGATACACACACGCAAGTCATATCGACGCTTTAAATCAGAATA
TTGCAGAGCTTTCTGACAACATCAATGTGTCATTTGAATTTTTCCCACCAAGCAGTGAGAAGATGGAAGAGACCCTGTGG
AATTCTGTTCACCGTCTTAAAACACTTCAACCTAAATTTGTATCAGTAACCTATGGTGCAAACTCGGGTGAGCGTGATCG
TACCCACTCAATCATTAAAGAAATTAAAAACCAAACAGGCTTAGTTGCTGCACCACACTTAACGTGTATTGATGCTAGCC
GCGAAGAGCTGATTCAAATTGCCGACGATTACTGGGCAAATGGCATTGAAAGTATTGTTGCGTTGCGCGGTGATATTCCA
GCAGGCGGTGGTGCGCCAGATATGTATGCGTCTGATTTAGTCGAGCTGCTTAAATCTCGTCACGACTTTGATATATCAGT
AGCGGCATTCCCTGAGGTTCACCCTGAAGCAAAAAGCGCTCAATCTGATCTTATTAACCTAAAGCGTAAAGTGGATGCGG
GTGCAAACCGTGCAATCACTCAGTTCTTCTTCGATGTCGAAAGCTACTTACGTTTTCGTGACCGTTGTGTGGCGGCTGGC
GTTGACGTAGAGATTGTACCGGGTATCTTGCCGGTTTCTAACTTCAAGCAAGCGTCTCGTTTTGCTGCGATGAATAACGT
AAAAGTACCAGGTTGGATGGCGAAGCAGTTCGAAGGCTTGGATGATGATCCAACAACTCGTCAGTTAGTCGGTGCTAGCC
AAGCGATCGATATGGTTCGTACGCTAAGCCGTGAAGGTGTGAAAGATTTCCACTTTTACACGCTAAACCGTGCAGAAATG
ACTTATGCACTTTGCCATACGTTAGGTGTTCGCCCACAGGTTGCTGCGCTGTAA

Upstream 100 bases:

>100_bases
AAGCACGATGTGTTTTCAGTCTTTTTTCTACATTATTTCTTCTTTGCGACCCACCTATCGGCTTGAGCTTTTCTCACTAT
CTACTTGAAAAAAATTCATA

Downstream 100 bases:

>100_bases
GTAAAGCCCAAGCGCTTGTAAAAGCTTCTGGTAGTAACATTTCAAATCCTATAGATACAAAAAAGGCTTGGACTCTAAGA
GTTCAAGCCTTTTACTTTTC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MDLLTLNRIQFILETYRRLNVARDLRLFSGCFCHRESKMGYTHASHIDALNQNIAELSDNINVSFEFFPPSSEKMEETLW
NSVHRLKTLQPKFVSVTYGANSGERDRTHSIIKEIKNQTGLVAAPHLTCIDASREELIQIADDYWANGIESIVALRGDIP
AGGGAPDMYASDLVELLKSRHDFDISVAAFPEVHPEAKSAQSDLINLKRKVDAGANRAITQFFFDVESYLRFRDRCVAAG
VDVEIVPGILPVSNFKQASRFAAMNNVKVPGWMAKQFEGLDDDPTTRQLVGASQAIDMVRTLSREGVKDFHFYTLNRAEM
TYALCHTLGVRPQVAAL

Sequences:

>Translated_337_residues
MDLLTLNRIQFILETYRRLNVARDLRLFSGCFCHRESKMGYTHASHIDALNQNIAELSDNINVSFEFFPPSSEKMEETLW
NSVHRLKTLQPKFVSVTYGANSGERDRTHSIIKEIKNQTGLVAAPHLTCIDASREELIQIADDYWANGIESIVALRGDIP
AGGGAPDMYASDLVELLKSRHDFDISVAAFPEVHPEAKSAQSDLINLKRKVDAGANRAITQFFFDVESYLRFRDRCVAAG
VDVEIVPGILPVSNFKQASRFAAMNNVKVPGWMAKQFEGLDDDPTTRQLVGASQAIDMVRTLSREGVKDFHFYTLNRAEM
TYALCHTLGVRPQVAAL
>Mature_337_residues
MDLLTLNRIQFILETYRRLNVARDLRLFSGCFCHRESKMGYTHASHIDALNQNIAELSDNINVSFEFFPPSSEKMEETLW
NSVHRLKTLQPKFVSVTYGANSGERDRTHSIIKEIKNQTGLVAAPHLTCIDASREELIQIADDYWANGIESIVALRGDIP
AGGGAPDMYASDLVELLKSRHDFDISVAAFPEVHPEAKSAQSDLINLKRKVDAGANRAITQFFFDVESYLRFRDRCVAAG
VDVEIVPGILPVSNFKQASRFAAMNNVKVPGWMAKQFEGLDDDPTTRQLVGASQAIDMVRTLSREGVKDFHFYTLNRAEM
TYALCHTLGVRPQVAAL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=281, Percent_Identity=33.4519572953737, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=76.530612244898, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI25147441, Length=298, Percent_Identity=30.2013422818792, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25147436, Length=298, Percent_Identity=30.2013422818792, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=278, Percent_Identity=31.294964028777, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=28.5211267605634, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24662658, Length=227, Percent_Identity=26.431718061674, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 37740; Mature: 37740

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLTLNRIQFILETYRRLNVARDLRLFSGCFCHRESKMGYTHASHIDALNQNIAELSDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
INVSFEFFPPSSEKMEETLWNSVHRLKTLQPKFVSVTYGANSGERDRTHSIIKEIKNQTG
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
LVAAPHLTCIDASREELIQIADDYWANGIESIVALRGDIPAGGGAPDMYASDLVELLKSR
EEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
HDFDISVAAFPEVHPEAKSAQSDLINLKRKVDAGANRAITQFFFDVESYLRFRDRCVAAG
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
VDVEIVPGILPVSNFKQASRFAAMNNVKVPGWMAKQFEGLDDDPTTRQLVGASQAIDMVR
CCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TLSREGVKDFHFYTLNRAEMTYALCHTLGVRPQVAAL
HHHHCCCCHHEEEEECHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MDLLTLNRIQFILETYRRLNVARDLRLFSGCFCHRESKMGYTHASHIDALNQNIAELSDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
INVSFEFFPPSSEKMEETLWNSVHRLKTLQPKFVSVTYGANSGERDRTHSIIKEIKNQTG
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
LVAAPHLTCIDASREELIQIADDYWANGIESIVALRGDIPAGGGAPDMYASDLVELLKSR
EEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
HDFDISVAAFPEVHPEAKSAQSDLINLKRKVDAGANRAITQFFFDVESYLRFRDRCVAAG
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
VDVEIVPGILPVSNFKQASRFAAMNNVKVPGWMAKQFEGLDDDPTTRQLVGASQAIDMVR
CCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
TLSREGVKDFHFYTLNRAEMTYALCHTLGVRPQVAAL
HHHHCCCCHHEEEEECHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]