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The map label for this gene is metB [H]

Identifier: 218710811

GI number: 218710811

Start: 3047343

End: 3048560

Strand: Reverse

Name: metB [H]

Synonym: VS_2894

Alternate gene names: 218710811

Gene position: 3048560-3047343 (Counterclockwise)

Preceding gene: 218710813

Following gene: 218710810

Centisome position: 92.4

GC content: 46.8

Gene sequence:

>1218_bases
GTGATTATCAGACGCAAAACTTGTAAGGAAGCACCTATGAGCAGCCGGAAGCCAGCAACCATCGCAGTACGTACTGGCAT
CGAGTCAGACACGCAACACCATGCCGTTGTCCCACCTATTTATCTTTCGACTAACTATGGGTTTCCCGCTTTTGGTGAAG
TGCCAAAGTATGATTATACCCGCTCTGGTAACCCAAATCGCGGTCTATTAGAAACGGCACTATTTGAGCTTGAGTCTGGT
AAAGGCGCGGTTGTCACTAACTGTGGTACGTCGGCACTTAACTTATGGGTATCCGCTTTCTTAGGTGGCGATGATCTTAT
TATTGCACCGCACGACTGCTATGGCGGTACTTACCGTCTGTTTAACACACGCTCGCTCAAAGGTGACTTCAAAGTTCTGT
TTGTTGATCAATCGGATCAAGCTGCACTGGATGCGGCGATCGCGCTTAAGCCAAAGTTGATCTTAATCGAAACGCCATCG
AATCCATTGGTTCGTGTGGTTGATATTGCAGAAACTTGCCGTAAAGCGAAAGAAGTAGGTGCTCTGGTTGCTGTCGACAA
CACATTTTTGACGCCTGTGTTCCAAAAGCCTTTAGAGCTGGGTGCGGATTTTGTTATCCACTCAACCACTAAATACATCA
ACGGACACTCAGATGTCATTGGTGGTGTTGTAGTTACTAAGACAGAAGAGCACGCTGAAGAGCTAGCATGGTGGGGTAAC
TGTATTGGTGCAACTGGCACACCATTTGATAGCTACATGACGCTACGTGGTATTCGTACGCTAGGTGCACGTATGCGCGT
TCACGAAGAGAGCTCACGTGAAATTCTGACGGCTCTGCAACAACAAGATCTGGTGGGTACGATTTACCACCCAAGCCTGC
CTGAACACCCGGGTCATGATATTGCCAAGAAACAGCAATCAGGTTTTGGCTCAATGTTGAGCTTTGAATTTGCGGGTTCA
TTCGAGGCACTTAAATATTTTGTGGATAAATTAGAGCTATTTTCTTTGGCTGAATCATTGGGCGGTGTTGAAAGCCTGAT
TTGTCATCCAGCGTCGATGACTCACCGTGCTATGGGCGAAGAAGCATTGGCAGAAGCGGGTGTTTCTCAACAATTACTGC
GCCTTTCTGTTGGCCTTGAAGATGCAGAAGACTTGATTGATGATCTCAAGCAAGCGTTTGAAAAAACACAAGGCTTTATC
GCAGAAGGGGAGGTTTAA

Upstream 100 bases:

>100_bases
CAAAGACATATAGACGTCCGGAAGTGTTGACGTCTCACGTTTATGAAAGTAAAGTGAGTAACATATATCTAACACAGCCA
CAAAATGCCGACAAGCAGCT

Downstream 100 bases:

>100_bases
TAATGACAACCTTTCGCCAGCTACATAAATTTGGTGGCAGCAGTTTAGCGAACCCAGAGTGTTACCAACGCGTGGTCAAT
ATTCTTAGAGAATACTCATC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MIIRRKTCKEAPMSSRKPATIAVRTGIESDTQHHAVVPPIYLSTNYGFPAFGEVPKYDYTRSGNPNRGLLETALFELESG
KGAVVTNCGTSALNLWVSAFLGGDDLIIAPHDCYGGTYRLFNTRSLKGDFKVLFVDQSDQAALDAAIALKPKLILIETPS
NPLVRVVDIAETCRKAKEVGALVAVDNTFLTPVFQKPLELGADFVIHSTTKYINGHSDVIGGVVVTKTEEHAEELAWWGN
CIGATGTPFDSYMTLRGIRTLGARMRVHEESSREILTALQQQDLVGTIYHPSLPEHPGHDIAKKQQSGFGSMLSFEFAGS
FEALKYFVDKLELFSLAESLGGVESLICHPASMTHRAMGEEALAEAGVSQQLLRLSVGLEDAEDLIDDLKQAFEKTQGFI
AEGEV

Sequences:

>Translated_405_residues
MIIRRKTCKEAPMSSRKPATIAVRTGIESDTQHHAVVPPIYLSTNYGFPAFGEVPKYDYTRSGNPNRGLLETALFELESG
KGAVVTNCGTSALNLWVSAFLGGDDLIIAPHDCYGGTYRLFNTRSLKGDFKVLFVDQSDQAALDAAIALKPKLILIETPS
NPLVRVVDIAETCRKAKEVGALVAVDNTFLTPVFQKPLELGADFVIHSTTKYINGHSDVIGGVVVTKTEEHAEELAWWGN
CIGATGTPFDSYMTLRGIRTLGARMRVHEESSREILTALQQQDLVGTIYHPSLPEHPGHDIAKKQQSGFGSMLSFEFAGS
FEALKYFVDKLELFSLAESLGGVESLICHPASMTHRAMGEEALAEAGVSQQLLRLSVGLEDAEDLIDDLKQAFEKTQGFI
AEGEV
>Mature_405_residues
MIIRRKTCKEAPMSSRKPATIAVRTGIESDTQHHAVVPPIYLSTNYGFPAFGEVPKYDYTRSGNPNRGLLETALFELESG
KGAVVTNCGTSALNLWVSAFLGGDDLIIAPHDCYGGTYRLFNTRSLKGDFKVLFVDQSDQAALDAAIALKPKLILIETPS
NPLVRVVDIAETCRKAKEVGALVAVDNTFLTPVFQKPLELGADFVIHSTTKYINGHSDVIGGVVVTKTEEHAEELAWWGN
CIGATGTPFDSYMTLRGIRTLGARMRVHEESSREILTALQQQDLVGTIYHPSLPEHPGHDIAKKQQSGFGSMLSFEFAGS
FEALKYFVDKLELFSLAESLGGVESLICHPASMTHRAMGEEALAEAGVSQQLLRLSVGLEDAEDLIDDLKQAFEKTQGFI
AEGEV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=39.5027624309392, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=36.7403314917127, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=34.6260387811634, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=57.6315789473684, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789383, Length=343, Percent_Identity=30.9037900874636, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=365, Percent_Identity=39.7260273972603, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17560056, Length=379, Percent_Identity=41.1609498680739, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=34.5945945945946, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=395, Percent_Identity=38.7341772151899, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=34.390243902439, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=430, Percent_Identity=30, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=396, Percent_Identity=29.5454545454545, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=25.4658385093168, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323557, Length=267, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322970, Length=272, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=380, Percent_Identity=40.7894736842105, Blast_Score=281, Evalue=8e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 44107; Mature: 44107

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIRRKTCKEAPMSSRKPATIAVRTGIESDTQHHAVVPPIYLSTNYGFPAFGEVPKYDYT
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCEEEECCCCCCCCCCCCCCCCC
RSGNPNRGLLETALFELESGKGAVVTNCGTSALNLWVSAFLGGDDLIIAPHDCYGGTYRL
CCCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
FNTRSLKGDFKVLFVDQSDQAALDAAIALKPKLILIETPSNPLVRVVDIAETCRKAKEVG
EECCCCCCCEEEEEECCCCCHHHHHHEEECCEEEEEECCCCCCEEEHHHHHHHHHHHHCC
ALVAVDNTFLTPVFQKPLELGADFVIHSTTKYINGHSDVIGGVVVTKTEEHAEELAWWGN
EEEEECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCHHHCCEEEECCHHHHHHHHHHHC
CIGATGTPFDSYMTLRGIRTLGARMRVHEESSREILTALQQQDLVGTIYHPSLPEHPGHD
CCCCCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCH
IAKKQQSGFGSMLSFEFAGSFEALKYFVDKLELFSLAESLGGVESLICHPASMTHRAMGE
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCH
EALAEAGVSQQLLRLSVGLEDAEDLIDDLKQAFEKTQGFIAEGEV
HHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure
MIIRRKTCKEAPMSSRKPATIAVRTGIESDTQHHAVVPPIYLSTNYGFPAFGEVPKYDYT
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCEEEECCCCCCCCCCCCCCCCC
RSGNPNRGLLETALFELESGKGAVVTNCGTSALNLWVSAFLGGDDLIIAPHDCYGGTYRL
CCCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
FNTRSLKGDFKVLFVDQSDQAALDAAIALKPKLILIETPSNPLVRVVDIAETCRKAKEVG
EECCCCCCCEEEEEECCCCCHHHHHHEEECCEEEEEECCCCCCEEEHHHHHHHHHHHHCC
ALVAVDNTFLTPVFQKPLELGADFVIHSTTKYINGHSDVIGGVVVTKTEEHAEELAWWGN
EEEEECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCHHHCCEEEECCHHHHHHHHHHHC
CIGATGTPFDSYMTLRGIRTLGARMRVHEESSREILTALQQQDLVGTIYHPSLPEHPGHD
CCCCCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCH
IAKKQQSGFGSMLSFEFAGSFEALKYFVDKLELFSLAESLGGVESLICHPASMTHRAMGE
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCH
EALAEAGVSQQLLRLSVGLEDAEDLIDDLKQAFEKTQGFIAEGEV
HHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]