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The map label for this gene is iaaA [H]

Identifier: 218710762

GI number: 218710762

Start: 2989721

End: 2990686

Strand: Reverse

Name: iaaA [H]

Synonym: VS_2845

Alternate gene names: 218710762

Gene position: 2990686-2989721 (Counterclockwise)

Preceding gene: 218710763

Following gene: 218710759

Centisome position: 90.65

GC content: 45.96

Gene sequence:

>966_bases
ATGTCACAGCCTTTTTCAATTGCCATTCATGGTGGTGCAGGCACCATCATACGAGAGCAAATGAGTGATGAATTAAAAAC
GGGTATTACCGAGGCTTTGGAAAAATCGGTTTTGGCTGGCTATCAAGTATTACAGTCGGGTGGCGATGCGCTAGATGCAG
TGGTTGCTTCAGTTAAGGTGATGGAAGACAGTCCCCACTTTAATGCCGGTAAAGGTTCTGTTCTGACTCATGATGAATTT
GTTGAGATGGATGCTTCTGTGATGCACGGTCGTGAAATGGATGCGGGGGCTATTGCCGGTGTCCGCCATATCAAAAATCC
AATTGAGCTTGCGCGTGATGTGATGCTTAAAAGCGACCATGTGCTGCTGATTGGTGAAGGCGCCGAGAAGTTTGCGTTTG
AGCATGACCATACTTTCACCGAGCAAGATTACTTCTTTACTGAGCGTCGTTATGACCAACTTCAGTCGATGAAAGAGAAA
GGTATTTTTGCTTTGTCTGAAGCGAAATATGATGAAGAGCAAGCAGATAAATATCCAGATGATAAAAAGTACGGCACGGT
TGGCGCGGTCGCATTAGACCAAGCCGGAAACTTAGCAGCCGCAACCAGCACTGGTGGCGTAACCAATAAAAAATACGGTC
GTGTGGGAGATTCTCCAATCATCGGTGCAGGCACTGTTGCTGAAAATGGCAACGTGGCCGTTTCAACAACAGGTATGGGG
GAGTTCTTCCTTAGAAAGATGGTTGCCAGTGATGTGGCGGCACGAATGCGTTATCTCAAGGAAGATGTGCATACGGCTTG
TGAAACGATTATTCAAGGTGAATTGAAAACCATGGGTGGTGAAGGAGGCTTGATTGCTATCGATGGACAAGGTGATATTC
ATTTCGGTATGAACAGTTCAGGAATGTACCGTGCGAGTGTTGATACTAATGGCTGCGTGGAAGTAAAAATTTATGCCGAT
GACTAA

Upstream 100 bases:

>100_bases
TAAGCGTTCGTAAATAGTTTGGTTTTCGCTATCAGACATCAAACCATGGATGTGGTATTGATAGCACAACATTATAAGTC
GACGTCAGTGGAGAGTTTGT

Downstream 100 bases:

>100_bases
GTTTGTATTGGCTTAACTGACTTTAGTTGAATTAGCGCAGATTAAACAAACAAAAGGGCGACAGATAAGTATCTGTCGCC
CTTTTGTTTTTCTATGTCCC

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSQPFSIAIHGGAGTIIREQMSDELKTGITEALEKSVLAGYQVLQSGGDALDAVVASVKVMEDSPHFNAGKGSVLTHDEF
VEMDASVMHGREMDAGAIAGVRHIKNPIELARDVMLKSDHVLLIGEGAEKFAFEHDHTFTEQDYFFTERRYDQLQSMKEK
GIFALSEAKYDEEQADKYPDDKKYGTVGAVALDQAGNLAAATSTGGVTNKKYGRVGDSPIIGAGTVAENGNVAVSTTGMG
EFFLRKMVASDVAARMRYLKEDVHTACETIIQGELKTMGGEGGLIAIDGQGDIHFGMNSSGMYRASVDTNGCVEVKIYAD
D

Sequences:

>Translated_321_residues
MSQPFSIAIHGGAGTIIREQMSDELKTGITEALEKSVLAGYQVLQSGGDALDAVVASVKVMEDSPHFNAGKGSVLTHDEF
VEMDASVMHGREMDAGAIAGVRHIKNPIELARDVMLKSDHVLLIGEGAEKFAFEHDHTFTEQDYFFTERRYDQLQSMKEK
GIFALSEAKYDEEQADKYPDDKKYGTVGAVALDQAGNLAAATSTGGVTNKKYGRVGDSPIIGAGTVAENGNVAVSTTGMG
EFFLRKMVASDVAARMRYLKEDVHTACETIIQGELKTMGGEGGLIAIDGQGDIHFGMNSSGMYRASVDTNGCVEVKIYAD
D
>Mature_320_residues
SQPFSIAIHGGAGTIIREQMSDELKTGITEALEKSVLAGYQVLQSGGDALDAVVASVKVMEDSPHFNAGKGSVLTHDEFV
EMDASVMHGREMDAGAIAGVRHIKNPIELARDVMLKSDHVLLIGEGAEKFAFEHDHTFTEQDYFFTERRYDQLQSMKEKG
IFALSEAKYDEEQADKYPDDKKYGTVGAVALDQAGNLAAATSTGGVTNKKYGRVGDSPIIGAGTVAENGNVAVSTTGMGE
FFLRKMVASDVAARMRYLKEDVHTACETIIQGELKTMGGEGGLIAIDGQGDIHFGMNSSGMYRASVDTNGCVEVKIYADD

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=304, Percent_Identity=40.1315789473684, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI145275200, Length=304, Percent_Identity=40.1315789473684, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI98991769, Length=291, Percent_Identity=31.6151202749141, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI285002251, Length=261, Percent_Identity=31.8007662835249, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1787050, Length=301, Percent_Identity=50.4983388704319, Blast_Score=278, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI32564649, Length=277, Percent_Identity=25.2707581227437, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18921183, Length=309, Percent_Identity=35.2750809061489, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=30.9701492537313, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI221330102, Length=242, Percent_Identity=32.6446280991736, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652135, Length=242, Percent_Identity=32.6446280991736, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 34451; Mature: 34320

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPFSIAIHGGAGTIIREQMSDELKTGITEALEKSVLAGYQVLQSGGDALDAVVASVKV
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHE
MEDSPHFNAGKGSVLTHDEFVEMDASVMHGREMDAGAIAGVRHIKNPIELARDVMLKSDH
ECCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
VLLIGEGAEKFAFEHDHTFTEQDYFFTERRYDQLQSMKEKGIFALSEAKYDEEQADKYPD
EEEEECCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCC
DKKYGTVGAVALDQAGNLAAATSTGGVTNKKYGRVGDSPIIGAGTVAENGNVAVSTTGMG
CCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEECCEEECCCCEEEEECCCH
EFFLRKMVASDVAARMRYLKEDVHTACETIIQGELKTMGGEGGLIAIDGQGDIHFGMNSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
GMYRASVDTNGCVEVKIYADD
CEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure 
SQPFSIAIHGGAGTIIREQMSDELKTGITEALEKSVLAGYQVLQSGGDALDAVVASVKV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHE
MEDSPHFNAGKGSVLTHDEFVEMDASVMHGREMDAGAIAGVRHIKNPIELARDVMLKSDH
ECCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
VLLIGEGAEKFAFEHDHTFTEQDYFFTERRYDQLQSMKEKGIFALSEAKYDEEQADKYPD
EEEEECCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCC
DKKYGTVGAVALDQAGNLAAATSTGGVTNKKYGRVGDSPIIGAGTVAENGNVAVSTTGMG
CCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEECCEEECCCCEEEEECCCH
EFFLRKMVASDVAARMRYLKEDVHTACETIIQGELKTMGGEGGLIAIDGQGDIHFGMNSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
GMYRASVDTNGCVEVKIYADD
CEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]