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The map label for this gene is slyD [H]

Identifier: 218710763

GI number: 218710763

Start: 2990922

End: 2991512

Strand: Reverse

Name: slyD [H]

Synonym: VS_2846

Alternate gene names: 218710763

Gene position: 2991512-2990922 (Counterclockwise)

Preceding gene: 218710764

Following gene: 218710762

Centisome position: 90.67

GC content: 48.39

Gene sequence:

>591_bases
ATGAAAATTGAAAAGAACGTAGTAGTTAGTGTTGCATATCAAGTGAAACTTGAAGATGGCGTAGTAGTTGACCAATCAAC
TGCAGAAGCTCCACTAGATTACCTTCACGGTCACAACAACCTAATTACAGGTCTTGAAAAAGAGCTTGAAGGCAAAGTCG
CTGGCGACAAGTTCTCAGCAACTGTTACTCCAGAAGACGCTTACGGCGAGCACAACGATGACCTAGTTCAACGTGTTCCT
GCAGACGTATTCCAAGGTGTTGAGCAAATCGAAGTTGGCATGCGTTTCCTAGCGGATACTGACCAAGGTCCAATCCCAGT
TGAAGTTACTGAAGTAGATGGCGACGAAGTTGTTGTTGACGGTAACCACATGCTAGCTGGCCAAACTCTAACGTTTGACG
TTGAAGTTGTAGCGGTTCGTGAAGCGACTGAAGAAGAAGTTCAACACGGTCACGTACACCAAGAAGGCGGCTGTGGCGGT
CACGACCACGATCATGATCACGAAGGTGGTTGCTGTGGTGGCGAAGGCCATGACCACGCTGAAGAGAAAAAAGACGGCTG
CTGCGGCGGCGGTAGCTGTGGTTCTCACTAA

Upstream 100 bases:

>100_bases
GAGTGATTGAGTTTCGTCAATTGATCCCCATAATATCCGGGTACAGAATTTTTCCTAAGCTCAACTGTTCGAGCTTAGTC
CAAACCTCCTTGGAGCTCTC

Downstream 100 bases:

>100_bases
TACCGAATTAACGGTTTAGTTTAAGAATATTGAAAACCTCCCTTGTGGAGGTTTTTTTCGTTTAGGGTGCTAGCTTTTTG
AGTTGGCTTTTCGTCTACCA

Product: SlpA protein

Products: NA

Alternate protein names: PPIase; Metallochaperone slyD [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKIEKNVVVSVAYQVKLEDGVVVDQSTAEAPLDYLHGHNNLITGLEKELEGKVAGDKFSATVTPEDAYGEHNDDLVQRVP
ADVFQGVEQIEVGMRFLADTDQGPIPVEVTEVDGDEVVVDGNHMLAGQTLTFDVEVVAVREATEEEVQHGHVHQEGGCGG
HDHDHDHEGGCCGGEGHDHAEEKKDGCCGGGSCGSH

Sequences:

>Translated_196_residues
MKIEKNVVVSVAYQVKLEDGVVVDQSTAEAPLDYLHGHNNLITGLEKELEGKVAGDKFSATVTPEDAYGEHNDDLVQRVP
ADVFQGVEQIEVGMRFLADTDQGPIPVEVTEVDGDEVVVDGNHMLAGQTLTFDVEVVAVREATEEEVQHGHVHQEGGCGG
HDHDHDHEGGCCGGEGHDHAEEKKDGCCGGGSCGSH
>Mature_196_residues
MKIEKNVVVSVAYQVKLEDGVVVDQSTAEAPLDYLHGHNNLITGLEKELEGKVAGDKFSATVTPEDAYGEHNDDLVQRVP
ADVFQGVEQIEVGMRFLADTDQGPIPVEVTEVDGDEVVVDGNHMLAGQTLTFDVEVVAVREATEEEVQHGHVHQEGGCGG
HDHDHDHEGGCCGGEGHDHAEEKKDGCCGGGSCGSH

Specific function: Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity [H]

COG id: COG1047

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 2

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789748, Length=147, Percent_Identity=66.6666666666667, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1786211, Length=146, Percent_Identity=34.2465753424658, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179 [H]

Pfam domain/function: PF00254 FKBP_C [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20888; Mature: 20888

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKNVVVSVAYQVKLEDGVVVDQSTAEAPLDYLHGHNNLITGLEKELEGKVAGDKFSA
CCCCCCEEEEEEEEEEECCCEEEECCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCEEEE
TVTPEDAYGEHNDDLVQRVPADVFQGVEQIEVGMRFLADTDQGPIPVEVTEVDGDEVVVD
EECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEC
GNHMLAGQTLTFDVEVVAVREATEEEVQHGHVHQEGGCGGHDHDHDHEGGCCGGEGHDHA
CCEEEECCEEEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EEKKDGCCGGGSCGSH
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKIEKNVVVSVAYQVKLEDGVVVDQSTAEAPLDYLHGHNNLITGLEKELEGKVAGDKFSA
CCCCCCEEEEEEEEEEECCCEEEECCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCEEEE
TVTPEDAYGEHNDDLVQRVPADVFQGVEQIEVGMRFLADTDQGPIPVEVTEVDGDEVVVD
EECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEC
GNHMLAGQTLTFDVEVVAVREATEEEVQHGHVHQEGGCGGHDHDHDHEGGCCGGEGHDHA
CCEEEECCEEEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EEKKDGCCGGGSCGSH
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]