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The map label for this gene is lepA [H]

Identifier: 218710571

GI number: 218710571

Start: 2810546

End: 2812339

Strand: Reverse

Name: lepA [H]

Synonym: VS_2621

Alternate gene names: 218710571

Gene position: 2812339-2810546 (Counterclockwise)

Preceding gene: 218710572

Following gene: 218710570

Centisome position: 85.24

GC content: 45.43

Gene sequence:

>1794_bases
ATGAAGCACATTCGTAATTTTTCGATTATCGCCCACATCGACCACGGTAAGTCGACCCTTTCTGACCGCTTAATCCAAGT
TTGTGGAGGGTTAAGTGAACGTGAGATGGCAGCTCAAGTCCTCGATTCTATGGATATAGAACGCGAGCGTGGTATTACAA
TTAAAGCGCAGAGTGTGACTTTAGATTACAAAGCTAAAGATGGTGAAACTTACCAACTTAACTTTATCGATACCCCTGGA
CACGTAGACTTCTCTTATGAAGTATCTCGCTCACTAGCCGCTTGTGAAGGCGCGCTTCTTGTAGTGGATGCCGGCCAAGG
TGTTGAAGCTCAAACTCTAGCAAACTGTTACACCGCGATCGAAATGGAACTGGAAGTAGTGCCAATCTTGAACAAGATTG
ACTTACCTGCTGCTGAACCTGAACGAGTTGCTGAAGAAATCGAAGAGATCGTTGGTATCGATGCGATGGAAGCGACGCGT
TGTTCTGCAAAAACCGGTATCGGTGTTGATGACGTTCTTGAAAACATCGTAACGGCTATCCCGCCACCGGAAGGTGATCC
AGAAGCGCCACTACAAGCTCTGATCATTGACTCTTGGTTCGATAACTACTTAGGCGTTGTTTCTTTGGTTCGTATCAAGA
ACGGTAAGCTGAAGAAGAACGACAAGATTAAAGTAATGTCGACAGACCAAGTTTGGGGTGTTGACCGTCTAGGTATCTTC
ACACCTAAGCAAATCGACACAACTGAGCTAAATACTGGCGAAGTTGGCTGGGTTGTTTGTGGTATTAAAGACATCCTTGG
CGCACCTGTTGGTGATACGTTGACGCTTGCAAAAGGCGGCAGCACAGAACGTCTACCTGGTTTCCAAAAAGTGAAGCCTC
AGGTATACGCGGGCCTGTTCCCAGTATCGTCTGATGACTACGAAAACTTCCGTGACGCACTAGGCAAACTAAGCCTGAAC
GATGCATCACTATTTTACGAACCAGAAAGTTCAGCAGCACTTGGCTTTGGTTTCCGTTGTGGCTTCTTAGGAATGCTTCA
CATGGAGATCATCCAAGAGCGTTTAGAGCGTGAATACGACCTAGACCTGATTACAACAGCTCCAACAGTAGTGTACGAAG
TTGTAAAAACAGATAAAACGGTTCTTTACGTTGATAGCCCGGCTAAGCTGCCTGCTATTAATGACTTAGAAGAAATTCGT
GAACCGATTGCTCGCTGTAATATTCTGGTACCTTCGGATTACCTAGGCAACGTGATCACACTGTGTGTTGAGAAGCGTGG
CGTGCAAGTCGACATGGTTTATCACGGTAACCAAGTTGCTGTGACGTACGATCTTCCAATGGCTGAGGTGGTTCTAGACT
TCTTCGACCGTCTGAAGTCAACGTCTCGCGGGTACGCATCATTGGATTACAACTTCCAACGTTACGAAGCATCAAACATG
GTACGTGTAGATGTATTGTTGAATGGTGAAACGGTTGATGCACTGGCGATCATTACACACAAAGATATTGCCCAGTCTCG
TGGTCGTCTACTGGTTGAGAAGATGAAAGAATTCATCCCTCGCCAGATGTTTGATATCGCGATTCAAGCCGCGATTGGTA
ACCACATTATTGCTCGTTCTACAGTGAAACAACTGCGTAAGAACGTAATCGCAAAATGTTACGGTGGTGATATCAGTCGT
AAGAAGAAACTTCTTAAGAAACAAAAAGAAGGTAAGAAGCGTATGAAGCAGATCGGTAACGTTGAACTGCCTCAAGAGGC
ATTCTTAGCAATCCTTCATGTTGGCAAAGACTAG

Upstream 100 bases:

>100_bases
GCCAACTTGATTGAAATGCCGCCACCAATGCTGACTGTAGATTGGACGCGTTGCGGCTTTCTTATTATCCCTATTCAAAG
AGTTTAGTCACACCAAGCCT

Downstream 100 bases:

>100_bases
GTATCAATCGCACTAACTATCTGCTGAACATTACTGGTTAAAATCGTTTATTTCTTTGTTGCAAAGATTGCAATTAGGCC
CACTAGTCACTGCACTTTGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSEREMAAQVLDSMDIERERGITIKAQSVTLDYKAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMELEVVPILNKIDLPAAEPERVAEEIEEIVGIDAMEATR
CSAKTGIGVDDVLENIVTAIPPPEGDPEAPLQALIIDSWFDNYLGVVSLVRIKNGKLKKNDKIKVMSTDQVWGVDRLGIF
TPKQIDTTELNTGEVGWVVCGIKDILGAPVGDTLTLAKGGSTERLPGFQKVKPQVYAGLFPVSSDDYENFRDALGKLSLN
DASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVVKTDKTVLYVDSPAKLPAINDLEEIR
EPIARCNILVPSDYLGNVITLCVEKRGVQVDMVYHGNQVAVTYDLPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASNM
VRVDVLLNGETVDALAIITHKDIAQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARSTVKQLRKNVIAKCYGGDISR
KKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD

Sequences:

>Translated_597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSEREMAAQVLDSMDIERERGITIKAQSVTLDYKAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMELEVVPILNKIDLPAAEPERVAEEIEEIVGIDAMEATR
CSAKTGIGVDDVLENIVTAIPPPEGDPEAPLQALIIDSWFDNYLGVVSLVRIKNGKLKKNDKIKVMSTDQVWGVDRLGIF
TPKQIDTTELNTGEVGWVVCGIKDILGAPVGDTLTLAKGGSTERLPGFQKVKPQVYAGLFPVSSDDYENFRDALGKLSLN
DASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVVKTDKTVLYVDSPAKLPAINDLEEIR
EPIARCNILVPSDYLGNVITLCVEKRGVQVDMVYHGNQVAVTYDLPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASNM
VRVDVLLNGETVDALAIITHKDIAQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARSTVKQLRKNVIAKCYGGDISR
KKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
>Mature_597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSEREMAAQVLDSMDIERERGITIKAQSVTLDYKAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMELEVVPILNKIDLPAAEPERVAEEIEEIVGIDAMEATR
CSAKTGIGVDDVLENIVTAIPPPEGDPEAPLQALIIDSWFDNYLGVVSLVRIKNGKLKKNDKIKVMSTDQVWGVDRLGIF
TPKQIDTTELNTGEVGWVVCGIKDILGAPVGDTLTLAKGGSTERLPGFQKVKPQVYAGLFPVSSDDYENFRDALGKLSLN
DASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVVKTDKTVLYVDSPAKLPAINDLEEIR
EPIARCNILVPSDYLGNVITLCVEKRGVQVDMVYHGNQVAVTYDLPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASNM
VRVDVLLNGETVDALAIITHKDIAQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARSTVKQLRKNVIAKCYGGDISR
KKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=48.0865224625624, Blast_Score=602, Evalue=1e-172,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=45.5223880597015, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI25306283, Length=175, Percent_Identity=41.1428571428571, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI19923640, Length=175, Percent_Identity=41.1428571428571, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306287, Length=175, Percent_Identity=41.1428571428571, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=34.8101265822785, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=44.1441441441441, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=44.1441441441441, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=44.1441441441441, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=34.1317365269461, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=34.1317365269461, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729339, Length=261, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI53729337, Length=261, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=84.7571189279732, Blast_Score=1021, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=506, Percent_Identity=26.6798418972332, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=36.1290322580645, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790835, Length=151, Percent_Identity=31.1258278145695, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=29.4642857142857, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.4918032786885, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17556745, Length=468, Percent_Identity=25.2136752136752, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17506493, Length=225, Percent_Identity=31.1111111111111, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=34.9315068493151, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=38.5185185185185, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=38.5185185185185, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.8421052631579, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=610, Percent_Identity=45.4098360655738, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=42.3611111111111, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=42.3611111111111, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=34.9726775956284, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=39.3103448275862, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=338, Percent_Identity=26.3313609467456, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=271, Percent_Identity=25.0922509225092, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=271, Percent_Identity=25.0922509225092, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.7603993344426, Blast_Score=540, Evalue=1e-153,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=44.6043165467626, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=35.4037267080745, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=36.4197530864198, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=36.4197530864198, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=36.4197530864198, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.255033557047, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=35.5555555555556, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28572034, Length=224, Percent_Identity=29.9107142857143, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921738, Length=244, Percent_Identity=28.2786885245902, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66101; Mature: 66101

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSEREMAAQVLDSMDIERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHE
EMELEVVPILNKIDLPAAEPERVAEEIEEIVGIDAMEATRCSAKTGIGVDDVLENIVTAI
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHC
PPPEGDPEAPLQALIIDSWFDNYLGVVSLVRIKNGKLKKNDKIKVMSTDQVWGVDRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCCCCCCCCCCCC
TPKQIDTTELNTGEVGWVVCGIKDILGAPVGDTLTLAKGGSTERLPGFQKVKPQVYAGLF
CCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCCHHHEEEE
PVSSDDYENFRDALGKLSLNDASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVVKTDKTVLYVDSPAKLPAINDLEEIREPIARCNILVPSDYLGNVIT
CEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHCEEECCCHHHHHHHH
LCVEKRGVQVDMVYHGNQVAVTYDLPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASNM
HHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCE
VRVDVLLNGETVDALAIITHKDIAQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKQLRKNVIAKCYGGDISRKKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSEREMAAQVLDSMDIERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHE
EMELEVVPILNKIDLPAAEPERVAEEIEEIVGIDAMEATRCSAKTGIGVDDVLENIVTAI
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHC
PPPEGDPEAPLQALIIDSWFDNYLGVVSLVRIKNGKLKKNDKIKVMSTDQVWGVDRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCCCCCCCCCCCC
TPKQIDTTELNTGEVGWVVCGIKDILGAPVGDTLTLAKGGSTERLPGFQKVKPQVYAGLF
CCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCCHHHEEEE
PVSSDDYENFRDALGKLSLNDASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVVKTDKTVLYVDSPAKLPAINDLEEIREPIARCNILVPSDYLGNVIT
CEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHCEEECCCHHHHHHHH
LCVEKRGVQVDMVYHGNQVAVTYDLPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASNM
HHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCE
VRVDVLLNGETVDALAIITHKDIAQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKQLRKNVIAKCYGGDISRKKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA