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The map label for this gene is lpd [H]

Identifier: 218710501

GI number: 218710501

Start: 2730181

End: 2731611

Strand: Reverse

Name: lpd [H]

Synonym: VS_2540

Alternate gene names: 218710501

Gene position: 2731611-2730181 (Counterclockwise)

Preceding gene: 218710502

Following gene: 218710499

Centisome position: 82.79

GC content: 45.35

Gene sequence:

>1431_bases
ATGAGCAAAGAAATTAAAGCCCAAGTTGTTGTACTTGGTTCAGGTCCTGCTGGTTACTCAGCGGCATTCCGTTGTGCGGA
TTTAGGTCTAGAAACAGTACTAGTTGAACGTTACAGCACTCTTGGTGGTGTATGTCTAAACGTTGGTTGTATTCCATCAA
AAGCACTTCTTCACGTATCTAAAGTAATTGAAGAAGCAAAAGCGATGGCAGAGCACGGCGTTGTATTCGGCGAGCCACAG
ACGGACATCAACAAGATCCGTATCTGGAAAGAAAAAGTAGTTGATCAACTGACTGGCGGTCTTGGCGGTATGGCTAAGAT
GCGTAACGTTACAGTTGTTAACGGTTTTGGTAAGTTCACTGGTCCTAACAGCATTCTTGTTGAAGGCGAAGGCGAAGCAA
CGACAGTTAACTTCGACAACGCAATCATTGCTGCGGGTTCTCGCCCAATCAAACTTCCGTTCATCCCACATGAAGACCCA
CGTATTTGGGACTCTACGGATGCATTAGAGCTAAACGAAGTACCAGAAAAACTGCTTATCATGGGCGGTGGTATCATCGG
TCTTGAGATGGGTACGGTTTACCACTCTCTAGGTTCTAAAGTTGAAGTTGTTGAGATGTTCGATCAAGTTATCCCTGCTG
CGGATAAAGACATCGTTAAAGTCTTCACTAAGCGCATTAAAGATAAGTTCAAGCTAATGCTTGAAACTAAAGTGACTGCT
GTTGAAGCGAAAGAAGACGGTATCTATGTTTCAATGGAAGGCAAAAAAGCACCAGCTGAAGCTGAGCGCTACGATGCAGT
TCTTGTTGCTATCGGTCGTGTTCCAAACGGTGCACTTATCGACGCTGAAAAAGCAGGTATCGAAGTTGATGAGCGCGGTT
TCATCAATGTTGATAAGCAAATGCGTACTAACGTTCCTCACATCCACGCGATCGGTGATGTTGTTGGTCAACCAATGCTT
GCTCACAAAGGTGTGCATGAAGGTCACGTAGCTGCTGAAGTTATCTCTGGTAAGAAGCACTACTTCGATCCTAAAGTAAT
CCCATCAATTGCGTACACTGAGCCAGAAGTAGCTTGGGTAGGTAAGACTGAGAAAGAAGCGAAAGCGGAAGGCCTGAACT
ACGAAGTTGCTACCTTCCCTTGGGCTGCTTCTGGTCGTGCAATCGCTTCAGACTGTGCTGACGGTATGACTAAGATGATC
TTCGATAAAGAGACTCATCGCGTAATCGGTGGTGCTGTTGTTGGTACTAATGGTGGTGAACTTCTTGGCGAAATCGGCCT
AGCAATCGAAATGGGTTGTGATGCAGAAGATATCGCACTGACTATCCACGCTCACCCAACTCTACACGAGTCTGTTGGTC
TTGCTGCAGAAGTATTCGAAGGTTCAATCACTGACCTTCCAAACAAAAAAGCAGTGAAAAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
ACACTGTTAACATCTCTGTAAAATGTTTCCTGTTTGAAAGCCCAATAATTTTAAGAACATCTACTCAGCCTGTTAGGGAT
AATGACTACAAGAGGTCACA

Downstream 100 bases:

>100_bases
TTCTTCTTTAGCCACTCGCTAATGTTTTTAAAAGCCGCTGATTCGTCAGCGGCTTTTTTATGTCTGAAGAAAAAATGGTC
GTCATGCCTGTAAGAGATCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKEIKAQVVVLGSGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVSKVIEEAKAMAEHGVVFGEPQ
TDINKIRIWKEKVVDQLTGGLGGMAKMRNVTVVNGFGKFTGPNSILVEGEGEATTVNFDNAIIAAGSRPIKLPFIPHEDP
RIWDSTDALELNEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVFTKRIKDKFKLMLETKVTA
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALIDAEKAGIEVDERGFINVDKQMRTNVPHIHAIGDVVGQPML
AHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGLNYEVATFPWAASGRAIASDCADGMTKMI
FDKETHRVIGGAVVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK

Sequences:

>Translated_476_residues
MSKEIKAQVVVLGSGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVSKVIEEAKAMAEHGVVFGEPQ
TDINKIRIWKEKVVDQLTGGLGGMAKMRNVTVVNGFGKFTGPNSILVEGEGEATTVNFDNAIIAAGSRPIKLPFIPHEDP
RIWDSTDALELNEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVFTKRIKDKFKLMLETKVTA
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALIDAEKAGIEVDERGFINVDKQMRTNVPHIHAIGDVVGQPML
AHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGLNYEVATFPWAASGRAIASDCADGMTKMI
FDKETHRVIGGAVVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK
>Mature_475_residues
SKEIKAQVVVLGSGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVSKVIEEAKAMAEHGVVFGEPQT
DINKIRIWKEKVVDQLTGGLGGMAKMRNVTVVNGFGKFTGPNSILVEGEGEATTVNFDNAIIAAGSRPIKLPFIPHEDPR
IWDSTDALELNEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVFTKRIKDKFKLMLETKVTAV
EAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALIDAEKAGIEVDERGFINVDKQMRTNVPHIHAIGDVVGQPMLA
HKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGLNYEVATFPWAASGRAIASDCADGMTKMIF
DKETHRVIGGAVVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=456, Percent_Identity=43.421052631579, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI50301238, Length=448, Percent_Identity=27.2321428571429, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI148277065, Length=459, Percent_Identity=27.0152505446623, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519430, Length=459, Percent_Identity=27.0152505446623, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519428, Length=459, Percent_Identity=27.0152505446623, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519426, Length=459, Percent_Identity=27.0152505446623, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277071, Length=460, Percent_Identity=26.9565217391304, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=27.7899343544858, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=27.3364485981308, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI291045268, Length=446, Percent_Identity=26.2331838565022, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=86.7088607594937, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=28.6937901498929, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=29.2307692307692, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=28.1531531531532, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=453, Percent_Identity=40.1766004415011, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.3109243697479, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=435, Percent_Identity=25.5172413793103, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=25, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=25, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.4473684210526, Blast_Score=310, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.9230769230769, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=452, Percent_Identity=27.4336283185841, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21358499, Length=453, Percent_Identity=41.5011037527594, Blast_Score=331, Evalue=8e-91,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=26.6524520255864, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=26.6524520255864, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=26.6524520255864, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17737741, Length=380, Percent_Identity=26.0526315789474, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50948; Mature: 50817

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIKAQVVVLGSGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVS
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAMAEHGVVFGEPQTDINKIRIWKEKVVDQLTGGLGGMAKMRNVTVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCEEEEEECCCCCC
GPNSILVEGEGEATTVNFDNAIIAAGSRPIKLPFIPHEDPRIWDSTDALELNEVPEKLLI
CCCEEEEECCCCEEEEECCCEEEECCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVFTKRIKDKFKLMLETKVTA
ECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEE
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALIDAEKAGIEVDERGFINVDKQ
EEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCEEEHHHCCCEECCCCCEEECHH
MRTNVPHIHAIGDVVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKEAKAEGLNYEVATFPWAASGRAIASDCADGMTKMIFDKETHRVIGGAVVGTNGGE
CCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKEIKAQVVVLGSGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVS
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAMAEHGVVFGEPQTDINKIRIWKEKVVDQLTGGLGGMAKMRNVTVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCEEEEEECCCCCC
GPNSILVEGEGEATTVNFDNAIIAAGSRPIKLPFIPHEDPRIWDSTDALELNEVPEKLLI
CCCEEEEECCCCEEEEECCCEEEECCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVFTKRIKDKFKLMLETKVTA
ECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEE
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALIDAEKAGIEVDERGFINVDKQ
EEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCEEEHHHCCCEECCCCCEEECHH
MRTNVPHIHAIGDVVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKEAKAEGLNYEVATFPWAASGRAIASDCADGMTKMIFDKETHRVIGGAVVGTNGGE
CCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]