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The map label for this gene is aceF [H]

Identifier: 218710502

GI number: 218710502

Start: 2731854

End: 2733728

Strand: Reverse

Name: aceF [H]

Synonym: VS_2541

Alternate gene names: 218710502

Gene position: 2733728-2731854 (Counterclockwise)

Preceding gene: 218710503

Following gene: 218710501

Centisome position: 82.86

GC content: 47.25

Gene sequence:

>1875_bases
ATGACAATCGAAATTAATGTACCAGACATCGGTGCTGACGAGGTTGAAGTAACTGAGATTCTTGTAAACGTTGGCGACAA
GGTTGAAGAAGAACAGTCACTGATCACTGTTGAAGGCGACAAAGCTTCAATGGAAGTTCCTGCGTCTCAAGCGGGTATCG
TTAAAGAAATCAAAATAGCAGAAGGCGATTCTGTTTCTACTGGTTCTCTTATCATGATCTTCGAAGCGGAAGGTGCAGCA
GCACCGACTGCGCCAGCAGTTGAAGCGGCGGCACCAGTTGCAGCAGCTCCTGCAGCGGCAGCTGAGCTTAAAGAAGTTCA
CGTTCCTGATATCGGCGGTGATGAAGTTGAAGTAACTGAAATCATGGTAGCTATTGGCGACGCAGTAGAAGAAGAGCAAT
CTCTTCTTACTGTTGAAGGTGACAAGGCTTCAATGGAAGTTCCTGCACCATTTGCTGGTATCGTTAAAGAAATCAAGATC
GCTTCTGGTGATTCAGTATCTACTGGTTCTCTAGTAATGGTATTTGAAGTGGCAGGTTCTGGCGCTCCAGTTGCCGCAGC
TCCTGCTCCAGCGGCGGCACCAGTTGCAGCAGCATCTGCTGAGAAAGAAGTGAACGTTCCTGATATCGGTGGCGACGAAG
TAGAAGTTACTGAAATCATGGTAGCGGTTGGCGATACAGTAGAAGAAGAGCAATCTCTAATTACTGTTGAAGGCGACAAA
GCTTCAATGGAAGTGCCTGCACCATTCGCTGGTACAGTAAAAGAAATCAAGATTGCAGCTGGCGACAAAGTGTCAACAGG
CTCTTCAATCATGACTTTCGTTGTTGAAGGCGCAGCTCCAGTAGCAGTAGCGGCTCCAGCTCCAGCACAAGCTCCGGCAG
CAGCACCTGCACCTAAAGCAGAAGCAGTAGCTCCAGCAGCTGGCGACTTCCAAGAGAACGGCGAGTACGCTCACGCATCT
CCAGTTGTTCGTCGTCTAGCTCGTGAATTCGGTGTAAACCTAGCGAAAGTTAAAGGTACTGGTCGTAAGAGTCGCGTACT
TAAAGAAGACGTTCAGTCTTACGTTAAAGATGCACTTAAGCGTCTTGAGTCTGGTGCTACAGCATCTGGCAAAGGCGGCG
ACGGTTCTGCTCTTGGTCTACTACCGTGGCCAAAAGTTGACTTCAGCAAGTTCGGCGAAACGGAAGTTCAGAAGCTTTCT
AAGATTAAGAAGATCTCTGGCGCTAACCTACACCGTAACTGGGTAATGATCCCGCACGTTACACAGTGGGACAACGCAGA
CATCACTGAGCTAGAAGCATTCCGTAAAGAACAGAACGCAATCGAAGCGAAGAAAGACACTGGCATGAAGATCACTCCAC
TTGTGTTCATCATGAAAGCTGTTGCTAAAGCGTTAGAAGCATTCCCAGCGTTTAACTCTTCTCTTTCTGAAGATGGCGAA
AGCATCATTCTTAAGAAGTACGTAAACGTGGGTATCGCTGTTGATACACCAAACGGCCTAGTTGTTCCTGTTTTCAAAGA
CGTGAACAAGAAAGGCATTTACGAGCTATCTGAAGAGCTAATGGCTGTTTCTAAGAAAGCACGTTCTGGTAAGCTAACAG
CGGCAGACATGCAAGGCGGTTGTTTCACAATCTCTAGCCTTGGTGGTATTGGCGGTACTGCATTTACTCCAATCGTAAAT
GCTCCAGAAGTAGGTATCCTAGGTGTATCTAAGTCTGAAATTAAGCCTGTTTGGAATGGCAAAGAGTTCCAACCACGTCT
ACAGCTTCCACTGTCTCTATCATACGATCACCGTGTGATCGATGGTGCTGAAGGTGCACGCTTCATTACTTTCCTAAACA
GCGCACTATCTGACATTCGTCGTCTAGTACTGTAA

Upstream 100 bases:

>100_bases
AGAAATCAGTAGTTGCTGAAGCAATTGCTAAATTCGGCATCGACGCAGACAAAATTAACCCGCAATACGCATAAGACTGG
CATTAAGGTAGGTAAATACA

Downstream 100 bases:

>100_bases
TTGAGAAAGTAATTATTAAGGTGACTTTCGGGTCACCTTAATTCTTTATATAAAGACTATTTTTAGAGAACAATTTCCGG
CATTTCTCATAAACTGCGGG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MTIEINVPDIGADEVEVTEILVNVGDKVEEEQSLITVEGDKASMEVPASQAGIVKEIKIAEGDSVSTGSLIMIFEAEGAA
APTAPAVEAAAPVAAAPAAAAELKEVHVPDIGGDEVEVTEIMVAIGDAVEEEQSLLTVEGDKASMEVPAPFAGIVKEIKI
ASGDSVSTGSLVMVFEVAGSGAPVAAAPAPAAAPVAAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEGDK
ASMEVPAPFAGTVKEIKIAAGDKVSTGSSIMTFVVEGAAPVAVAAPAPAQAPAAAPAPKAEAVAPAAGDFQENGEYAHAS
PVVRRLAREFGVNLAKVKGTGRKSRVLKEDVQSYVKDALKRLESGATASGKGGDGSALGLLPWPKVDFSKFGETEVQKLS
KIKKISGANLHRNWVMIPHVTQWDNADITELEAFRKEQNAIEAKKDTGMKITPLVFIMKAVAKALEAFPAFNSSLSEDGE
SIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARSGKLTAADMQGGCFTISSLGGIGGTAFTPIVN
APEVGILGVSKSEIKPVWNGKEFQPRLQLPLSLSYDHRVIDGAEGARFITFLNSALSDIRRLVL

Sequences:

>Translated_624_residues
MTIEINVPDIGADEVEVTEILVNVGDKVEEEQSLITVEGDKASMEVPASQAGIVKEIKIAEGDSVSTGSLIMIFEAEGAA
APTAPAVEAAAPVAAAPAAAAELKEVHVPDIGGDEVEVTEIMVAIGDAVEEEQSLLTVEGDKASMEVPAPFAGIVKEIKI
ASGDSVSTGSLVMVFEVAGSGAPVAAAPAPAAAPVAAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEGDK
ASMEVPAPFAGTVKEIKIAAGDKVSTGSSIMTFVVEGAAPVAVAAPAPAQAPAAAPAPKAEAVAPAAGDFQENGEYAHAS
PVVRRLAREFGVNLAKVKGTGRKSRVLKEDVQSYVKDALKRLESGATASGKGGDGSALGLLPWPKVDFSKFGETEVQKLS
KIKKISGANLHRNWVMIPHVTQWDNADITELEAFRKEQNAIEAKKDTGMKITPLVFIMKAVAKALEAFPAFNSSLSEDGE
SIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARSGKLTAADMQGGCFTISSLGGIGGTAFTPIVN
APEVGILGVSKSEIKPVWNGKEFQPRLQLPLSLSYDHRVIDGAEGARFITFLNSALSDIRRLVL
>Mature_623_residues
TIEINVPDIGADEVEVTEILVNVGDKVEEEQSLITVEGDKASMEVPASQAGIVKEIKIAEGDSVSTGSLIMIFEAEGAAA
PTAPAVEAAAPVAAAPAAAAELKEVHVPDIGGDEVEVTEIMVAIGDAVEEEQSLLTVEGDKASMEVPAPFAGIVKEIKIA
SGDSVSTGSLVMVFEVAGSGAPVAAAPAPAAAPVAAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEGDKA
SMEVPAPFAGTVKEIKIAAGDKVSTGSSIMTFVVEGAAPVAVAAPAPAQAPAAAPAPKAEAVAPAAGDFQENGEYAHASP
VVRRLAREFGVNLAKVKGTGRKSRVLKEDVQSYVKDALKRLESGATASGKGGDGSALGLLPWPKVDFSKFGETEVQKLSK
IKKISGANLHRNWVMIPHVTQWDNADITELEAFRKEQNAIEAKKDTGMKITPLVFIMKAVAKALEAFPAFNSSLSEDGES
IILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARSGKLTAADMQGGCFTISSLGGIGGTAFTPIVNA
PEVGILGVSKSEIKPVWNGKEFQPRLQLPLSLSYDHRVIDGAEGARFITFLNSALSDIRRLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=31.4942528735632, Blast_Score=170, Evalue=5e-42,
Organism=Homo sapiens, GI31711992, Length=325, Percent_Identity=33.8461538461538, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=30.5676855895196, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI203098816, Length=230, Percent_Identity=32.1739130434783, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI203098753, Length=230, Percent_Identity=32.1739130434783, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=35.8974358974359, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786305, Length=634, Percent_Identity=70.0315457413249, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=30.0248138957816, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=416, Percent_Identity=29.8076923076923, Blast_Score=168, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=432, Percent_Identity=28.9351851851852, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=209, Percent_Identity=36.8421052631579, Blast_Score=127, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=29.1139240506329, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=417, Percent_Identity=28.2973621103118, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=347, Percent_Identity=29.1066282420749, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=31.554524361949, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=33.0316742081448, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=29.8701298701299, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129315, Length=213, Percent_Identity=31.924882629108, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 64803; Mature: 64672

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEINVPDIGADEVEVTEILVNVGDKVEEEQSLITVEGDKASMEVPASQAGIVKEIKIA
CEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEEC
EGDSVSTGSLIMIFEAEGAAAPTAPAVEAAAPVAAAPAAAAELKEVHVPDIGGDEVEVTE
CCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEHHH
IMVAIGDAVEEEQSLLTVEGDKASMEVPAPFAGIVKEIKIASGDSVSTGSLVMVFEVAGS
HHHHHHHHHHCCCCEEEEECCCCCEECCCCHHHHHHHHEECCCCCCCCCCEEEEEEECCC
GAPVAAAPAPAAAPVAAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEGDK
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEHEEEEEHHCCCCCCCCCEEEEECCC
ASMEVPAPFAGTVKEIKIAAGDKVSTGSSIMTFVVEGAAPVAVAAPAPAQAPAAAPAPKA
CCEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
EAVAPAAGDFQENGEYAHASPVVRRLAREFGVNLAKVKGTGRKSRVLKEDVQSYVKDALK
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
RLESGATASGKGGDGSALGLLPWPKVDFSKFGETEVQKLSKIKKISGANLHRNWVMIPHV
HHHCCCCCCCCCCCCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCC
TQWDNADITELEAFRKEQNAIEAKKDTGMKITPLVFIMKAVAKALEAFPAFNSSLSEDGE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCH
SIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARSGKLTAADMQGG
HEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
CFTISSLGGIGGTAFTPIVNAPEVGILGVSKSEIKPVWNGKEFQPRLQLPLSLSYDHRVI
EEEEECCCCCCCCHHCCCCCCCCCEEEECCHHHCCCCCCCCCCCCEECCCEEECCCCEEE
DGAEGARFITFLNSALSDIRRLVL
CCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TIEINVPDIGADEVEVTEILVNVGDKVEEEQSLITVEGDKASMEVPASQAGIVKEIKIA
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEEC
EGDSVSTGSLIMIFEAEGAAAPTAPAVEAAAPVAAAPAAAAELKEVHVPDIGGDEVEVTE
CCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEHHH
IMVAIGDAVEEEQSLLTVEGDKASMEVPAPFAGIVKEIKIASGDSVSTGSLVMVFEVAGS
HHHHHHHHHHCCCCEEEEECCCCCEECCCCHHHHHHHHEECCCCCCCCCCEEEEEEECCC
GAPVAAAPAPAAAPVAAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEGDK
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEHEEEEEHHCCCCCCCCCEEEEECCC
ASMEVPAPFAGTVKEIKIAAGDKVSTGSSIMTFVVEGAAPVAVAAPAPAQAPAAAPAPKA
CCEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
EAVAPAAGDFQENGEYAHASPVVRRLAREFGVNLAKVKGTGRKSRVLKEDVQSYVKDALK
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
RLESGATASGKGGDGSALGLLPWPKVDFSKFGETEVQKLSKIKKISGANLHRNWVMIPHV
HHHCCCCCCCCCCCCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCC
TQWDNADITELEAFRKEQNAIEAKKDTGMKITPLVFIMKAVAKALEAFPAFNSSLSEDGE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHCCCH
SIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARSGKLTAADMQGG
HEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
CFTISSLGGIGGTAFTPIVNAPEVGILGVSKSEIKPVWNGKEFQPRLQLPLSLSYDHRVI
EEEEECCCCCCCCHHCCCCCCCCCEEEECCHHHCCCCCCCCCCCCEECCCEEECCCCEEE
DGAEGARFITFLNSALSDIRRLVL
CCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]