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The map label for this gene is folK [H]

Identifier: 218710492

GI number: 218710492

Start: 2721328

End: 2721813

Strand: Direct

Name: folK [H]

Synonym: VS_2531

Alternate gene names: 218710492

Gene position: 2721328-2721813 (Clockwise)

Preceding gene: 218710491

Following gene: 218710493

Centisome position: 82.48

GC content: 46.71

Gene sequence:

>486_bases
ATGATAACGGCTTACATTGCGGTCGGCAGTAATCTTGCCGACCCCGTTAGCCAAGCAAACTTGGCTATCGAAACGCTAAA
GAATCTTCCGCGAACAACGTTTGTTGCGACCTCACAGCTATATAGTAGCACTCCAATGGGGCCGAAAAACCAACCCGACT
ACATCAACGCGGTAGTAGCAATTAAAACAGAATTAACGCCAATTGAACTGCTTGATTGCACTCAAAAGATCGAGCAAGAA
CAAGGGCGCGTCCGTAAAGACGAGCGTTGGGGGCCAAGAACCTTGGATCTCGATATCGTGTTATACGGCAATGAGGTGAT
CGATTCAGAGCGCTTAACCGTTCCTCATTACGGAATGAAAGAAAGAGAGTTTGTGCTCTACCCGCTTGCTGAAATCGCAC
CAAGTTTACAACTCCCTGATGGGACTGAGCTGACAGAACTACTAAAAATAGTAGATAAGAACGGGCTCAATGTTTGGCAG
CAATAG

Upstream 100 bases:

>100_bases
GCAACATGCGTCAAGCGATGGCTAATGACGTAGCAGCACCATCAAAGTCGGGTCATCGCCGCCGTAAGACTTACAGAAAC
AAAAAAAGTAAGCAATCAGA

Downstream 100 bases:

>100_bases
CCAAGCGCATTAAGGAAAATCAATGAAAAAAGTAACCATTAACGACCTAATCAAGTGCAAACAAGAAGGCCGTAAATTCG
CGACGTCGACAGCTTATGAT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MITAYIAVGSNLADPVSQANLAIETLKNLPRTTFVATSQLYSSTPMGPKNQPDYINAVVAIKTELTPIELLDCTQKIEQE
QGRVRKDERWGPRTLDLDIVLYGNEVIDSERLTVPHYGMKEREFVLYPLAEIAPSLQLPDGTELTELLKIVDKNGLNVWQ
Q

Sequences:

>Translated_161_residues
MITAYIAVGSNLADPVSQANLAIETLKNLPRTTFVATSQLYSSTPMGPKNQPDYINAVVAIKTELTPIELLDCTQKIEQE
QGRVRKDERWGPRTLDLDIVLYGNEVIDSERLTVPHYGMKEREFVLYPLAEIAPSLQLPDGTELTELLKIVDKNGLNVWQ
Q
>Mature_161_residues
MITAYIAVGSNLADPVSQANLAIETLKNLPRTTFVATSQLYSSTPMGPKNQPDYINAVVAIKTELTPIELLDCTQKIEQE
QGRVRKDERWGPRTLDLDIVLYGNEVIDSERLTVPHYGMKEREFVLYPLAEIAPSLQLPDGTELTELLKIVDKNGLNVWQ
Q

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=57.2327044025157, Blast_Score=196, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6324073, Length=140, Percent_Identity=36.4285714285714, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18054; Mature: 18054

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAYIAVGSNLADPVSQANLAIETLKNLPRTTFVATSQLYSSTPMGPKNQPDYINAVVA
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCEEEEEE
IKTELTPIELLDCTQKIEQEQGRVRKDERWGPRTLDLDIVLYGNEVIDSERLTVPHYGMK
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCEEECCCCEECCCCCCC
EREFVLYPLAEIAPSLQLPDGTELTELLKIVDKNGLNVWQQ
CCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MITAYIAVGSNLADPVSQANLAIETLKNLPRTTFVATSQLYSSTPMGPKNQPDYINAVVA
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCEEEEEE
IKTELTPIELLDCTQKIEQEQGRVRKDERWGPRTLDLDIVLYGNEVIDSERLTVPHYGMK
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCEEECCCCEECCCCCCC
EREFVLYPLAEIAPSLQLPDGTELTELLKIVDKNGLNVWQQ
CCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]