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The map label for this gene is panB

Identifier: 218710493

GI number: 218710493

Start: 2721836

End: 2722630

Strand: Direct

Name: panB

Synonym: VS_2532

Alternate gene names: 218710493

Gene position: 2721836-2722630 (Clockwise)

Preceding gene: 218710492

Following gene: 218710494

Centisome position: 82.5

GC content: 45.16

Gene sequence:

>795_bases
ATGAAAAAAGTAACCATTAACGACCTAATCAAGTGCAAACAAGAAGGCCGTAAATTCGCGACGTCGACAGCTTATGATGC
GAGCTTTGCTCAATTATTCGAAAGCCAAGATATGCCAGTTCTACTTGTCGGTGACTCACTTGGCATGGTTCTACAAGGTC
GTAACGATACATTACCAGTAACCGTTGAAGATATTGCTTACCATACTCGCTCAGTACGTGCCGGTAGCCCAAACTGTCTT
CTTATGGCTGACATGCCATTCATGAGTTACGCCACACCAGAGCAAGCCTGTGAGAACGCAGCAACCTTAATGCGCGCTGG
TGCGAACATGGTGAAAATCGAAGGCGGAAGCTGGTTGATTGAAACTGTGAAGATGCTAACAGAACGTGCTGTACCAGTAT
GTGCACACTTAGGCTTAACGCCTCAATCGGTAAACATTTTTGGCGGTTACAAAGTTCAAGGTCGCGACAACGAGCAAGCC
GATAAAATGGTTGCTGATGCGCTAGCGCTACAAAATGCAGGTGCTCAAATCGTTCTTCTTGAATGTGTACCAGCTTCATT
AGCAAAAAGAATTACAGAAGCTTGTGACGTACCGGTTATCGGTATCGGCGCAGGTAATGTTACCGATGGTCAGATCTTGG
TTATGCATGACATGTTCGGTATTTCTGCGAACTACATGCCGAAATTCTCTAAGAATTTCCTAGCTGAAACAGGTGATATG
CGTAAAGCCGTCGCTCTATACAAAGAAGAAGTAGAGAGCGCACTTTTCCCTGATGATGCTCATACAATCGCTTAG

Upstream 100 bases:

>100_bases
CAACTCCCTGATGGGACTGAGCTGACAGAACTACTAAAAATAGTAGATAAGAACGGGCTCAATGTTTGGCAGCAATAGCC
AAGCGCATTAAGGAAAATCA

Downstream 100 bases:

>100_bases
GAGTAACTATGCAAACTTTTGCTGAGATAGCGGCTCTTCGTGAGCAGATTAAACAGTTTAAGCGTGATGGACGTACGGTT
GCTTTTGTCCCAACAATGGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKKVTINDLIKCKQEGRKFATSTAYDASFAQLFESQDMPVLLVGDSLGMVLQGRNDTLPVTVEDIAYHTRSVRAGSPNCL
LMADMPFMSYATPEQACENAATLMRAGANMVKIEGGSWLIETVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGRDNEQA
DKMVADALALQNAGAQIVLLECVPASLAKRITEACDVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
RKAVALYKEEVESALFPDDAHTIA

Sequences:

>Translated_264_residues
MKKVTINDLIKCKQEGRKFATSTAYDASFAQLFESQDMPVLLVGDSLGMVLQGRNDTLPVTVEDIAYHTRSVRAGSPNCL
LMADMPFMSYATPEQACENAATLMRAGANMVKIEGGSWLIETVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGRDNEQA
DKMVADALALQNAGAQIVLLECVPASLAKRITEACDVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
RKAVALYKEEVESALFPDDAHTIA
>Mature_264_residues
MKKVTINDLIKCKQEGRKFATSTAYDASFAQLFESQDMPVLLVGDSLGMVLQGRNDTLPVTVEDIAYHTRSVRAGSPNCL
LMADMPFMSYATPEQACENAATLMRAGANMVKIEGGSWLIETVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGRDNEQA
DKMVADALALQNAGAQIVLLECVPASLAKRITEACDVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
RKAVALYKEEVESALFPDDAHTIA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=70.722433460076, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=35.5311355311355, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_VIBSL (B7VK03)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418114.1
- GeneID:   7162066
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2532
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28485; Mature: 28485

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTINDLIKCKQEGRKFATSTAYDASFAQLFESQDMPVLLVGDSLGMVLQGRNDTLPV
CCCCCHHHHHHHHHHCHHHHCCCCCCHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCE
TVEDIAYHTRSVRAGSPNCLLMADMPFMSYATPEQACENAATLMRAGANMVKIEGGSWLI
EHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
ETVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGRDNEQADKMVADALALQNAGAQIVLL
HHHHHHHHHCCHHHHHCCCCCCCEEEEECEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
ECVPASLAKRITEACDVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
EECCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHH
RKAVALYKEEVESALFPDDAHTIA
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKVTINDLIKCKQEGRKFATSTAYDASFAQLFESQDMPVLLVGDSLGMVLQGRNDTLPV
CCCCCHHHHHHHHHHCHHHHCCCCCCHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCE
TVEDIAYHTRSVRAGSPNCLLMADMPFMSYATPEQACENAATLMRAGANMVKIEGGSWLI
EHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
ETVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGRDNEQADKMVADALALQNAGAQIVLL
HHHHHHHHHCCHHHHHCCCCCCCEEEEECEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
ECVPASLAKRITEACDVPVIGIGAGNVTDGQILVMHDMFGISANYMPKFSKNFLAETGDM
EECCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHH
RKAVALYKEEVESALFPDDAHTIA
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA