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The map label for this gene is pcnB [H]

Identifier: 218710491

GI number: 218710491

Start: 2719913

End: 2721331

Strand: Direct

Name: pcnB [H]

Synonym: VS_2530

Alternate gene names: 218710491

Gene position: 2719913-2721331 (Clockwise)

Preceding gene: 218710490

Following gene: 218710492

Centisome position: 82.44

GC content: 47.15

Gene sequence:

>1419_bases
CTGCACTACCACTTTGGACTAAGAGAAAAACAAAGCTGGCGATACCTACTTTGTTATAAACTAATGAATACAAACGACAA
TACCCCAAGCGAACAACGCGGATTCCACGAATTAGCTCTGAATATTTATACTCGTCAAGAGCACAATATTTCACGCAAGC
AGATCAGCGACAATGCACTTAAAGTGTTATATCGCCTGAATGGTGCGGGTTTTGACGCATTTCTAGTCGGTGGTGGTGTA
CGCGACATCTTATTAGGCTCTCAGCCAAAAGATTTTGATATCGCGACCAACGCAACGCCAGAGCAAATCAAGCACCTATT
CAGAAACTGTCGCCTTATCGGTCGACGTTTTCGCTTGGCACATATCATGTTTGGTCGTGACATCATTGAAGTAGCAACTT
TCCGTGGTCACCACCAAGAGCCATCGAAAAACGTTTCTGCGCAATCTGACGAAGGTATGTTGCTACGCGATAACGTGTAC
GGCAATGTTGATGAAGATGCAGAACGTCGTGACTTCACAATCAATGCGATGTACTACAACATTGCAGACTATAGCATCCA
CGATTACGCGGGTGGGGTAGAAGATTTAGAAGATAAGCTGATCCGCCTGATTGGCGACCCAGAAACGCGTTACCGCGAAG
ACCCAGTACGCATGCTACGCGCAATGCGCTTCGCCGCTAAACTGGATTTTGATATTGAGGAAGATACAGCCGACCCAATT
GAAGAGTTGGCACATCTTCTAAAAGATATCCCAGCAGCGCGCCTTTACGAAGAGTCTCTAAAATTACTGCAATCAGGTCA
CGGTTTAGAAACCTACCACTTGATGCGTGAGTACAACCTGTTCCAGCAGATGTTCCCTTCCGTAGCGGAGCACTTCACAG
AAGGCTACGACTCACATACCGAGCAAATGCTTGATTTGGTACTCGACTCGACTGACCTTCGTATCGAAGATGGCAAGCGA
GTAAACCCCGCGTTTATGTTTGCTGCAATGCTTTGGTACCCAATGAACAAGTTGGCAGACAAGCTTGTCGCTGAACAGGG
TATGGCGCACTACGATGCGATCATGGAAGCAAGTAACATCATTCTTGATCGACAAGTAAAATCAATTGCGATTCCTCGTC
GCCATACCGCAACCATTCGCGAAGTTTGGCAACTGCAACTACGACTGCCTCGCCGCAACGGTAAACGTGCCGTTCGCCTG
ATGGAACTCAACAAGTTCCGTGCCGGCTACGATTTCCTAGAAATGCGTGGCGAAATTGAAGGTGGCGAAACCAAAGAACT
AGCAAAATGGTGGGAGCGTTATCAAACAGCAGGACGCAACATGCGTCAAGCGATGGCTAATGACGTAGCAGCACCATCAA
AGTCGGGTCATCGCCGCCGTAAGACTTACAGAAACAAAAAAAGTAAGCAATCAGAATGA

Upstream 100 bases:

>100_bases
GCAATAACGATACATAAAAATACCCTAACAAATACCTGCGACTAAGCAGACGATGTTCTCAAATGGCTCGCGCTAAGCTA
TTATTAGCCGCAATTAAACT

Downstream 100 bases:

>100_bases
TAACGGCTTACATTGCGGTCGGCAGTAATCTTGCCGACCCCGTTAGCCAAGCAAACTTGGCTATCGAAACGCTAAAGAAT
CTTCCGCGAACAACGTTTGT

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MHYHFGLREKQSWRYLLCYKLMNTNDNTPSEQRGFHELALNIYTRQEHNISRKQISDNALKVLYRLNGAGFDAFLVGGGV
RDILLGSQPKDFDIATNATPEQIKHLFRNCRLIGRRFRLAHIMFGRDIIEVATFRGHHQEPSKNVSAQSDEGMLLRDNVY
GNVDEDAERRDFTINAMYYNIADYSIHDYAGGVEDLEDKLIRLIGDPETRYREDPVRMLRAMRFAAKLDFDIEEDTADPI
EELAHLLKDIPAARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPSVAEHFTEGYDSHTEQMLDLVLDSTDLRIEDGKR
VNPAFMFAAMLWYPMNKLADKLVAEQGMAHYDAIMEASNIILDRQVKSIAIPRRHTATIREVWQLQLRLPRRNGKRAVRL
MELNKFRAGYDFLEMRGEIEGGETKELAKWWERYQTAGRNMRQAMANDVAAPSKSGHRRRKTYRNKKSKQSE

Sequences:

>Translated_472_residues
MHYHFGLREKQSWRYLLCYKLMNTNDNTPSEQRGFHELALNIYTRQEHNISRKQISDNALKVLYRLNGAGFDAFLVGGGV
RDILLGSQPKDFDIATNATPEQIKHLFRNCRLIGRRFRLAHIMFGRDIIEVATFRGHHQEPSKNVSAQSDEGMLLRDNVY
GNVDEDAERRDFTINAMYYNIADYSIHDYAGGVEDLEDKLIRLIGDPETRYREDPVRMLRAMRFAAKLDFDIEEDTADPI
EELAHLLKDIPAARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPSVAEHFTEGYDSHTEQMLDLVLDSTDLRIEDGKR
VNPAFMFAAMLWYPMNKLADKLVAEQGMAHYDAIMEASNIILDRQVKSIAIPRRHTATIREVWQLQLRLPRRNGKRAVRL
MELNKFRAGYDFLEMRGEIEGGETKELAKWWERYQTAGRNMRQAMANDVAAPSKSGHRRRKTYRNKKSKQSE
>Mature_472_residues
MHYHFGLREKQSWRYLLCYKLMNTNDNTPSEQRGFHELALNIYTRQEHNISRKQISDNALKVLYRLNGAGFDAFLVGGGV
RDILLGSQPKDFDIATNATPEQIKHLFRNCRLIGRRFRLAHIMFGRDIIEVATFRGHHQEPSKNVSAQSDEGMLLRDNVY
GNVDEDAERRDFTINAMYYNIADYSIHDYAGGVEDLEDKLIRLIGDPETRYREDPVRMLRAMRFAAKLDFDIEEDTADPI
EELAHLLKDIPAARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPSVAEHFTEGYDSHTEQMLDLVLDSTDLRIEDGKR
VNPAFMFAAMLWYPMNKLADKLVAEQGMAHYDAIMEASNIILDRQVKSIAIPRRHTATIREVWQLQLRLPRRNGKRAVRL
MELNKFRAGYDFLEMRGEIEGGETKELAKWWERYQTAGRNMRQAMANDVAAPSKSGHRRRKTYRNKKSKQSE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=220, Percent_Identity=32.2727272727273, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI87081691, Length=436, Percent_Identity=55.045871559633, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1789436, Length=225, Percent_Identity=27.1111111111111, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281360112, Length=223, Percent_Identity=30.4932735426009, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357337, Length=223, Percent_Identity=30.4932735426009, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 55015; Mature: 55015

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYHFGLREKQSWRYLLCYKLMNTNDNTPSEQRGFHELALNIYTRQEHNISRKQISDNAL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KVLYRLNGAGFDAFLVGGGVRDILLGSQPKDFDIATNATPEQIKHLFRNCRLIGRRFRLA
HHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HIMFGRDIIEVATFRGHHQEPSKNVSAQSDEGMLLRDNVYGNVDEDAERRDFTINAMYYN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEE
IADYSIHDYAGGVEDLEDKLIRLIGDPETRYREDPVRMLRAMRFAAKLDFDIEEDTADPI
EECCCHHHHCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHH
EELAHLLKDIPAARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPSVAEHFTEGYDSHT
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EQMLDLVLDSTDLRIEDGKRVNPAFMFAAMLWYPMNKLADKLVAEQGMAHYDAIMEASNI
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
ILDRQVKSIAIPRRHTATIREVWQLQLRLPRRNGKRAVRLMELNKFRAGYDFLEMRGEIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCC
GGETKELAKWWERYQTAGRNMRQAMANDVAAPSKSGHRRRKTYRNKKSKQSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHYHFGLREKQSWRYLLCYKLMNTNDNTPSEQRGFHELALNIYTRQEHNISRKQISDNAL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KVLYRLNGAGFDAFLVGGGVRDILLGSQPKDFDIATNATPEQIKHLFRNCRLIGRRFRLA
HHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HIMFGRDIIEVATFRGHHQEPSKNVSAQSDEGMLLRDNVYGNVDEDAERRDFTINAMYYN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEE
IADYSIHDYAGGVEDLEDKLIRLIGDPETRYREDPVRMLRAMRFAAKLDFDIEEDTADPI
EECCCHHHHCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHH
EELAHLLKDIPAARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPSVAEHFTEGYDSHT
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EQMLDLVLDSTDLRIEDGKRVNPAFMFAAMLWYPMNKLADKLVAEQGMAHYDAIMEASNI
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
ILDRQVKSIAIPRRHTATIREVWQLQLRLPRRNGKRAVRLMELNKFRAGYDFLEMRGEIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCC
GGETKELAKWWERYQTAGRNMRQAMANDVAAPSKSGHRRRKTYRNKKSKQSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]