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The map label for this gene is ispA [H]

Identifier: 218710368

GI number: 218710368

Start: 2574522

End: 2575406

Strand: Direct

Name: ispA [H]

Synonym: VS_2405

Alternate gene names: 218710368

Gene position: 2574522-2575406 (Clockwise)

Preceding gene: 218710367

Following gene: 218710369

Centisome position: 78.03

GC content: 46.33

Gene sequence:

>885_bases
ATGATCGAGACGTTATTGTCTTATCAAGCACGTAATAACGAGCAACTTAACCTTTGGCTTGATCGCCTGCCACACCAAAA
TCAGAATCTCATTAACGCGATGCGTTATGGGTTACTTTTAGGCGGCAAACGCGCACGTCCGTTTCTTGTCTACATTACAG
GGGAAATGCTCGGTTGCACTGCGGAAGAACTCGACACTCCAGCTTCAGCAATCGAATGTATTCATGCCTATTCTCTGATT
CACGACGACCTTCCAGCAATGGACGACGACGAATTGCGTCGTGGCCATCAGACTTGTCACATTAAATACGATGAAGCAAC
GGCAATTTTAACTGGCGATGCACTACAAACTCTCGCGTTTACTATACTTGCGGAAGGCACATTAAGTGCTGACGGGGAAA
GCAATCGCGTTCGAATGATTCAACGCCTAGCAGAAGCGTCTGGTGCACAAGGTATGTGTATTGGACAAGCTCTTGATATT
GAAGCTGAAAACCGCTCTGTCACGCTAGAGGAGTTAGAAGAAGTTCACCGTAACAAGACTGGTGCTCTAATGAAGAGTGC
GATTCGTTTAGGTGCTCTAGCTGCTGGCGAAAAAGCGTTCGAAGTGATGCCTCAATTAGATAAGTACGCCGATGCAATCG
GGTTAGCCTTCCAAGTTCAGGATGATATTTTAGATATCATTAGCGATACCGAAACTTTGGGTAAACCACAGGGCTCTGAC
CAAGATTTGAACAAAAGCACCTACCCTTCTTTGTTAGGTTTAGAGGGCGCTCAAGAAAAAGCGCAAACTCTGCTACAGGA
AGCGCTTCAAGCTTTGGCTGCAATCCCATACAATACCCAGTTACTCGAAGAGTTCGCCCGATACGTCATCGAGCGCAAGA
ACTAA

Upstream 100 bases:

>100_bases
ACGCTGAACAGCGTGTTAGCATCCTGCTGAAAAATGATGAAAATGCAGAACTGAGTGACTTTAACCCACAACCTGAATAA
CAAATTGTATGAGATCCCCT

Downstream 100 bases:

>100_bases
GACAATAAGCGCGCATTACCTATGACTCTTGATATATCAAAGTACCCAACTCTTGCTTTGGCTGATAAGCCAGAGGATTT
GCGTCTACTTCCAAAAGAGA

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIETLLSYQARNNEQLNLWLDRLPHQNQNLINAMRYGLLLGGKRARPFLVYITGEMLGCTAEELDTPASAIECIHAYSLI
HDDLPAMDDDELRRGHQTCHIKYDEATAILTGDALQTLAFTILAEGTLSADGESNRVRMIQRLAEASGAQGMCIGQALDI
EAENRSVTLEELEEVHRNKTGALMKSAIRLGALAAGEKAFEVMPQLDKYADAIGLAFQVQDDILDIISDTETLGKPQGSD
QDLNKSTYPSLLGLEGAQEKAQTLLQEALQALAAIPYNTQLLEEFARYVIERKN

Sequences:

>Translated_294_residues
MIETLLSYQARNNEQLNLWLDRLPHQNQNLINAMRYGLLLGGKRARPFLVYITGEMLGCTAEELDTPASAIECIHAYSLI
HDDLPAMDDDELRRGHQTCHIKYDEATAILTGDALQTLAFTILAEGTLSADGESNRVRMIQRLAEASGAQGMCIGQALDI
EAENRSVTLEELEEVHRNKTGALMKSAIRLGALAAGEKAFEVMPQLDKYADAIGLAFQVQDDILDIISDTETLGKPQGSD
QDLNKSTYPSLLGLEGAQEKAQTLLQEALQALAAIPYNTQLLEEFARYVIERKN
>Mature_294_residues
MIETLLSYQARNNEQLNLWLDRLPHQNQNLINAMRYGLLLGGKRARPFLVYITGEMLGCTAEELDTPASAIECIHAYSLI
HDDLPAMDDDELRRGHQTCHIKYDEATAILTGDALQTLAFTILAEGTLSADGESNRVRMIQRLAEASGAQGMCIGQALDI
EAENRSVTLEELEEVHRNKTGALMKSAIRLGALAAGEKAFEVMPQLDKYADAIGLAFQVQDDILDIISDTETLGKPQGSD
QDLNKSTYPSLLGLEGAQEKAQTLLQEALQALAAIPYNTQLLEEFARYVIERKN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=285, Percent_Identity=57.8947368421053, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1789578, Length=280, Percent_Identity=29.2857142857143, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=203, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651612, Length=194, Percent_Identity=30.4123711340206, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32432; Mature: 32432

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETLLSYQARNNEQLNLWLDRLPHQNQNLINAMRYGLLLGGKRARPFLVYITGEMLGCT
CHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHCCC
AEELDTPASAIECIHAYSLIHDDLPAMDDDELRRGHQTCHIKYDEATAILTGDALQTLAF
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEECCCEEEEEHHHHHHHHH
TILAEGTLSADGESNRVRMIQRLAEASGAQGMCIGQALDIEAENRSVTLEELEEVHRNKT
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEECCCCCCCEEEHHHHHHHHCCHH
GALMKSAIRLGALAAGEKAFEVMPQLDKYADAIGLAFQVQDDILDIISDTETLGKPQGSD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCC
QDLNKSTYPSLLGLEGAQEKAQTLLQEALQALAAIPYNTQLLEEFARYVIERKN
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIETLLSYQARNNEQLNLWLDRLPHQNQNLINAMRYGLLLGGKRARPFLVYITGEMLGCT
CHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHCCC
AEELDTPASAIECIHAYSLIHDDLPAMDDDELRRGHQTCHIKYDEATAILTGDALQTLAF
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEECCCEEEEEHHHHHHHHH
TILAEGTLSADGESNRVRMIQRLAEASGAQGMCIGQALDIEAENRSVTLEELEEVHRNKT
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEECCCCCCCEEEHHHHHHHHCCHH
GALMKSAIRLGALAAGEKAFEVMPQLDKYADAIGLAFQVQDDILDIISDTETLGKPQGSD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCC
QDLNKSTYPSLLGLEGAQEKAQTLLQEALQALAAIPYNTQLLEEFARYVIERKN
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2089044; 9278503 [H]