Click here to switch to the map view.

The map label for this gene is rfaH [H]

Identifier: 218710232

GI number: 218710232

Start: 2435603

End: 2436109

Strand: Reverse

Name: rfaH [H]

Synonym: VS_2265

Alternate gene names: 218710232

Gene position: 2436109-2435603 (Counterclockwise)

Preceding gene: 218710234

Following gene: 218710227

Centisome position: 73.84

GC content: 42.8

Gene sequence:

>507_bases
ATGAAACGTTGGTATTTACTTTACTGTAAGCGTGGTGATCAAAAACGCGCTCAACAGCACCTAGAAAATCAGGGGGTAGA
GTGCTTTTACCCTCAAATAGAAGTCGAAAAGGTAGTGAGAGGGAAAGAGAAGCTGGTCCAAGAACCGCTGTTCCCATCCT
ATATCTTTGTCCGTTTTGATTATGAGCAAGGCCCAAGCTTTACTACCGTTCGTTCAACTCGTGGTGTTGTCGACTTTATT
AAGTTCGGTGCAAGGCCGTATGAAGTCCAAGGTGATTTGGTTTTTGAGCTAAAAGAGTTCGAGAAGTGTTGTAGTAGCGA
AGTTGAAGATTGCTGTGTTGAATTTGAGACAGGGCAAGTTGTCAAAATTAATAGTGGCCAGTTTGCGGGTGTTGAAGCTA
TTTACCATCAGAAAGATGGCGAAGCTCGATCCATTATGCTCGTAAAAATGATCAGCCAAGTGGTTCCGATCAGTATTGAA
AACGAAGCACTACAAGCTCACGTATAA

Upstream 100 bases:

>100_bases
GTTCCGCGTAATTTCTCGCTTATCAGGACTCATTTATTAACGGCGACTAAATTGTTTTAGGCTGTGGTTAAATGTCCAAA
GAACGAAGATGGCGAGTTAA

Downstream 100 bases:

>100_bases
GTCAAATATTAAAAAAGTAAAAACTAGGCTTAAAACAAAAAGGCGCATAATGCGCCTTTTTTAGTATTTGATTTTTCAAA
CGCTTAAGCGTTATAGGCGT

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MKRWYLLYCKRGDQKRAQQHLENQGVECFYPQIEVEKVVRGKEKLVQEPLFPSYIFVRFDYEQGPSFTTVRSTRGVVDFI
KFGARPYEVQGDLVFELKEFEKCCSSEVEDCCVEFETGQVVKINSGQFAGVEAIYHQKDGEARSIMLVKMISQVVPISIE
NEALQAHV

Sequences:

>Translated_168_residues
MKRWYLLYCKRGDQKRAQQHLENQGVECFYPQIEVEKVVRGKEKLVQEPLFPSYIFVRFDYEQGPSFTTVRSTRGVVDFI
KFGARPYEVQGDLVFELKEFEKCCSSEVEDCCVEFETGQVVKINSGQFAGVEAIYHQKDGEARSIMLVKMISQVVPISIE
NEALQAHV
>Mature_168_residues
MKRWYLLYCKRGDQKRAQQHLENQGVECFYPQIEVEKVVRGKEKLVQEPLFPSYIFVRFDYEQGPSFTTVRSTRGVVDFI
KFGARPYEVQGDLVFELKEFEKCCSSEVEDCCVEFETGQVVKINSGQFAGVEAIYHQKDGEARSIMLVKMISQVVPISIE
NEALQAHV

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=161, Percent_Identity=43.4782608695652, Blast_Score=142, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 19400; Mature: 19400

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWYLLYCKRGDQKRAQQHLENQGVECFYPQIEVEKVVRGKEKLVQEPLFPSYIFVRFD
CCCEEEEEEECCCHHHHHHHHHHCCCEEEECCCHHHHHHCCHHHHHHCCCCCCEEEEEEE
YEQGPSFTTVRSTRGVVDFIKFGARPYEVQGDLVFELKEFEKCCSSEVEDCCVEFETGQV
CCCCCCEEEEHHCCCHHHHHHHCCCCEEECCCEEEHHHHHHHHHCCHHHHHHEECCCCCE
VKINSGQFAGVEAIYHQKDGEARSIMLVKMISQVVPISIENEALQAHV
EEECCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCEECCCHHEECC
>Mature Secondary Structure
MKRWYLLYCKRGDQKRAQQHLENQGVECFYPQIEVEKVVRGKEKLVQEPLFPSYIFVRFD
CCCEEEEEEECCCHHHHHHHHHHCCCEEEECCCHHHHHHCCHHHHHHCCCCCCEEEEEEE
YEQGPSFTTVRSTRGVVDFIKFGARPYEVQGDLVFELKEFEKCCSSEVEDCCVEFETGQV
CCCCCCEEEEHHCCCHHHHHHHCCCCEEECCCEEEHHHHHHHHHCCHHHHHHEECCCCCE
VKINSGQFAGVEAIYHQKDGEARSIMLVKMISQVVPISIENEALQAHV
EEECCCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCEECCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]