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The map label for this gene is nagE [H]

Identifier: 218710227

GI number: 218710227

Start: 2427556

End: 2429151

Strand: Reverse

Name: nagE [H]

Synonym: VS_2260

Alternate gene names: 218710227

Gene position: 2429151-2427556 (Counterclockwise)

Preceding gene: 218710232

Following gene: 218710226

Centisome position: 73.63

GC content: 46.3

Gene sequence:

>1596_bases
ATGAATTCTAAAAAATCCTATAGGGGGAAACAACTGGTGAATATTCTAGGATATGCACAGAAGCTAGGTAAGGCATTAAT
GCTACCTATCGCAACACTTCCGATTGCGGCGCTTCTACTACGTTTAGGTCAAGGCGATCTACTTGATATTCCATTCATGG
CGCAAGCTGGTGGAGCTATCTTCGGTAACCTACCATTGCTATTTGGTCTAGGTATCGCGATTGGTCTTTCTAAAGACGGT
AACGGCGCAGCAGGTCTTGCTGGTGCAGTTGCTTACTTCGTACTAACAGCGACAGCAACAACAATTAACGCTGACGTAAA
CATGTCATTCTTTGGCGGTATCTTCGCAGGTATCATCGCAGGTCACTCTTACAACGCTTTCCATGCAACACGTCTTCCTG
AGTGGCTGGCTTTCTTTGCGGGTAAACGTTTAGTACCTATCATGGCTGGTCTATTTGCACTTGTTGCAGGTGCTGTGTCT
GGTGTGGTTTGGCCTGGTGTTCAATCTGGTCTAGACGCACTGGCTCACGCAGTATCAACGTCTGGCGCTATCGGTCAATT
CGTTTACGGTACTCTTAACCGTGCACTTATCCCTGTAGGTCTACACCACGTATTGAACTCATACTTCTGGTTCGGTATGG
GTACATGTCAAGAAATCATCGTTGCTGGTCAAGGCGCATTCGCTAACATCACTCAACTTTGTGTTGACCCATCACTAGCT
AAAACTCTAGTTGTTGGCCAAGAGCACACATTCACATTCGCTAACTCTGTAACTCCAGAAATCACTACTGTAGTTAAAGA
AGTGACTGAAACTGTTAAATCTGGTGACCTACACCGTTTCTTCGGTGGCGATAAAGGCGCTGGCGTATTCATGAACGGTT
TCTTCCCAGTAATGATGTTCGGTCTACCAGGTGCTGCACTTGCAATGTACCTAGCTGCTCCTGCTGAAAAACGCAGCCAA
GTTGGTGGCGCACTGTTCTCAGTTGCATTCTGTTCATTCCTAACAGGTATCACAGAGCCGCTAGAATTCATGTTCGTATT
CCTAGCTCCTGCTCTATACGCAATGCACGCTGTGTTTACAGGTCTGTCTCTAGTAGTTGCAAACATGTTTGGTACTCTGC
ACGGTTTCGGTTTCTCTGCTGGTCTTATCGACTTCGTATTGAACTGGGGTCTAGCAACTAAACCATTCGTACTACTACTA
ATCGGTCTTGGTTTCGGTGCTCTATACTTCTTCACTTTCTCTTTCGCAATCCGCGCTTTCAACTTGAAATCGCCAGGTCG
TGAAGATGATGACGAAGCTGTAGCCGCTCCTGCTGGCGACGCACCAAAAGGTGAAGTTGCACGTCAATACCTGAAAGCTC
TAGGTGGTCACGACAACCTAACTTCAATTGACGCTTGTATCACTCGTCTACGTCTAACTCTTAAAGACCGCTCTATCGCA
GATGAAGCTGTTCTTAAAAAGCTTGGCGCTAAAGGTGTGGTTAAACTAGGTGAGAACAACCTACAGGTTATCCTAGGCCC
ACTAGCTGAAATCGTAGCTGGCGAAATGAAAGCTATCGGTGCAGGTGAAGACCTATCTGATGTAAAACTTCCATAG

Upstream 100 bases:

>100_bases
GGTGATTAGGATCACAAATGGTAAGGTTTTAATTTGCGGGGCAAAATTAATTGAATACACTGAATAGGACTAAATTGAGC
GACTAAAATAAGTTTCTCAA

Downstream 100 bases:

>100_bases
TGACGGAAGTACTTAATTAAGAAGTAAAGTTACTCTTAAGTAAGTTTCAAATTGAAAGCCTCCCAAACGGGAGGCTTTTT
TGTGCCTACTGTTTAAGGTC

Product: PTS system N-acetylglucosamine-specific transporter subunit EIICBA

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MNSKKSYRGKQLVNILGYAQKLGKALMLPIATLPIAALLLRLGQGDLLDIPFMAQAGGAIFGNLPLLFGLGIAIGLSKDG
NGAAGLAGAVAYFVLTATATTINADVNMSFFGGIFAGIIAGHSYNAFHATRLPEWLAFFAGKRLVPIMAGLFALVAGAVS
GVVWPGVQSGLDALAHAVSTSGAIGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEIIVAGQGAFANITQLCVDPSLA
KTLVVGQEHTFTFANSVTPEITTVVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALAMYLAAPAEKRSQ
VGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVANMFGTLHGFGFSAGLIDFVLNWGLATKPFVLLL
IGLGFGALYFFTFSFAIRAFNLKSPGREDDDEAVAAPAGDAPKGEVARQYLKALGGHDNLTSIDACITRLRLTLKDRSIA
DEAVLKKLGAKGVVKLGENNLQVILGPLAEIVAGEMKAIGAGEDLSDVKLP

Sequences:

>Translated_531_residues
MNSKKSYRGKQLVNILGYAQKLGKALMLPIATLPIAALLLRLGQGDLLDIPFMAQAGGAIFGNLPLLFGLGIAIGLSKDG
NGAAGLAGAVAYFVLTATATTINADVNMSFFGGIFAGIIAGHSYNAFHATRLPEWLAFFAGKRLVPIMAGLFALVAGAVS
GVVWPGVQSGLDALAHAVSTSGAIGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEIIVAGQGAFANITQLCVDPSLA
KTLVVGQEHTFTFANSVTPEITTVVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALAMYLAAPAEKRSQ
VGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVANMFGTLHGFGFSAGLIDFVLNWGLATKPFVLLL
IGLGFGALYFFTFSFAIRAFNLKSPGREDDDEAVAAPAGDAPKGEVARQYLKALGGHDNLTSIDACITRLRLTLKDRSIA
DEAVLKKLGAKGVVKLGENNLQVILGPLAEIVAGEMKAIGAGEDLSDVKLP
>Mature_531_residues
MNSKKSYRGKQLVNILGYAQKLGKALMLPIATLPIAALLLRLGQGDLLDIPFMAQAGGAIFGNLPLLFGLGIAIGLSKDG
NGAAGLAGAVAYFVLTATATTINADVNMSFFGGIFAGIIAGHSYNAFHATRLPEWLAFFAGKRLVPIMAGLFALVAGAVS
GVVWPGVQSGLDALAHAVSTSGAIGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEIIVAGQGAFANITQLCVDPSLA
KTLVVGQEHTFTFANSVTPEITTVVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALAMYLAAPAEKRSQ
VGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVANMFGTLHGFGFSAGLIDFVLNWGLATKPFVLLL
IGLGFGALYFFTFSFAIRAFNLKSPGREDDDEAVAAPAGDAPKGEVARQYLKALGGHDNLTSIDACITRLRLTLKDRSIA
DEAVLKKLGAKGVVKLGENNLQVILGPLAEIVAGEMKAIGAGEDLSDVKLP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=516, Percent_Identity=47.8682170542636, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1787343, Length=523, Percent_Identity=36.1376673040153, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1787908, Length=555, Percent_Identity=33.5135135135135, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 55642; Mature: 55642

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKKSYRGKQLVNILGYAQKLGKALMLPIATLPIAALLLRLGQGDLLDIPFMAQAGGAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHCCCCC
FGNLPLLFGLGIAIGLSKDGNGAAGLAGAVAYFVLTATATTINADVNMSFFGGIFAGIIA
CCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
GHSYNAFHATRLPEWLAFFAGKRLVPIMAGLFALVAGAVSGVVWPGVQSGLDALAHAVST
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
SGAIGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEIIVAGQGAFANITQLCVDPSLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHHH
KTLVVGQEHTFTFANSVTPEITTVVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMF
HHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEHHHHHHHHH
GLPGAALAMYLAAPAEKRSQVGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFT
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSLVVANMFGTLHGFGFSAGLIDFVLNWGLATKPFVLLLIGLGFGALYFFTFSFAIRAF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLKSPGREDDDEAVAAPAGDAPKGEVARQYLKALGGHDNLTSIDACITRLRLTLKDRSIA
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHH
DEAVLKKLGAKGVVKLGENNLQVILGPLAEIVAGEMKAIGAGEDLSDVKLP
HHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNSKKSYRGKQLVNILGYAQKLGKALMLPIATLPIAALLLRLGQGDLLDIPFMAQAGGAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHCCCCC
FGNLPLLFGLGIAIGLSKDGNGAAGLAGAVAYFVLTATATTINADVNMSFFGGIFAGIIA
CCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
GHSYNAFHATRLPEWLAFFAGKRLVPIMAGLFALVAGAVSGVVWPGVQSGLDALAHAVST
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
SGAIGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEIIVAGQGAFANITQLCVDPSLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHHH
KTLVVGQEHTFTFANSVTPEITTVVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMF
HHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEHHHHHHHHH
GLPGAALAMYLAAPAEKRSQVGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFT
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSLVVANMFGTLHGFGFSAGLIDFVLNWGLATKPFVLLLIGLGFGALYFFTFSFAIRAF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLKSPGREDDDEAVAAPAGDAPKGEVARQYLKALGGHDNLTSIDACITRLRLTLKDRSIA
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHH
DEAVLKKLGAKGVVKLGENNLQVILGPLAEIVAGEMKAIGAGEDLSDVKLP
HHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]