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The map label for this gene is sapA [H]

Identifier: 218709974

GI number: 218709974

Start: 2140171

End: 2141793

Strand: Direct

Name: sapA [H]

Synonym: VS_1993

Alternate gene names: 218709974

Gene position: 2140171-2141793 (Clockwise)

Preceding gene: 218709973

Following gene: 218709975

Centisome position: 64.87

GC content: 44.36

Gene sequence:

>1623_bases
ATGAAAGCACTAATAAGACTATCACTGAGCATCTGCACGCTTGGCTTTTTAGCTGGATGTGGTGAAAGTGTCACTCACGA
GCAAATCCGTGAAAAAGGCTTCATTTATTGCGGCCAAGGTAATCCTAGTACCTTCAATCCACAATTGGTTGATAGTGGCA
TTACCTCAGAATCGCTGAGCCCTCAAATTTTCGATACCCTGCTCACTCTTGATCCTATGACTTACCGACCAAAACAGAAC
TTGGCAACAAGCTGGTCTGTCGATAAATCAGGTACAGAGTACACTTTCAAACTGCGACCAAATGTTGCGTTTCAAACCAC
AGATTGGTTTGCCCCAACCCGCACCATGAATGCACAGGATGTGGTCTTTAGTTTTGAACGAATTATTGATAGTTCAAACC
CATTCCATTATGTGGGTGGCGGTTTATATCCATGGTTCGCTGGCATCGACTTTCAAAACCTGATTGTTGAAATCACAGCG
GTGGATGACCTAACGGTGAAGTTCCAGTTAAGTCGCCCAGATAATTCATTTCTTAACAATATCGCCACCAGTTATGCGGT
TATCCACTCCAAAGAGTATGCCAACAAGCTGATTACCACAGATGAAAAAAATGAAATAGACTCCAAGCCCGTAGGCACAG
GCCCTTTCTACTTGGATGAATATCAAATTAATGATCTTGTACGCTTGAAAAAGAACAAGCATTACTGGAAGGGTGAAGTA
CAAATGGACCAAGTCGTATTTGATACTTCACAACGCGGTACAGGCACACTTGCCAAGCTTCTCCGCAGTGAGTGTGACGT
GTTGAACTCACCAATTTCTAGCCAGATCCCTATTATTCAAGCTCATGAAGAATTGAAGATTTCTGCCACACCTGCAGTAA
ATGTCTCTTTCATCGCGCTAAACACAGAACACCCTGCATTGCGTGACGCACGAGTACGTAAAGCATTGAACTTGGCTATT
AACCGTCAGAACATTCTTGATTCTGTGTACTACGGTACAGGTACAAAAGCCTACACGTTACTTCCCCCTACTTCTTGGGC
TTATCAAAAAGACAGCGTTCAAGTTCGTTATGACCGCAACTATGCACTCGCACTTCTTAGAGAGGCCGGTGTAGAACCAG
GGTTAGAGCTTTCAATGTGGGTGCCACTAGAGCCACGCGCATACAATCCAAGTCCAAGAAAAACGGCAGAACTGATCCAA
GCAAACTTGGCTGATATTGGTATTGAATTGAAACTCTATACCGACGACCGATTTGACCGAACACAGCTGTCTTCAATTTC
CTCAACAGATTTGTTACTCACAGGTTGGATTGGCAGTACGGGCGACCCAGACAACTTCTTACGTCCACTTCTATCATGTA
GTTCGGAACGCGCGGGATTAAACGTCTCTATGTGGTGCAACTCAGACTTTGATTTCCTTCTCGACTTGGCATTAGAGATT
AATCAACAAAGGCATCGCGTGAACTTGTATCGACAAGCCCAAAACATTCTTAATGAAGAAGTTCCTGTGATACCTCTCGC
TCACGGCGTGCAATTCCGTGTGCACGATCGTTCGCTAGCAGGTTTTAAACGCAGTCCTTTTAATGCTCAACCTTTTGATC
AAGTCAGAAGGGAGGTGAACTGA

Upstream 100 bases:

>100_bases
GTCCACACTTGATTATTATTTGGAGCCGAATAAATAAAATGTTAAATTGTCGGGATCTTGAGGCTCCTCTAACGCCTCGT
TTCGAAAAGTATTTCTTTTT

Downstream 100 bases:

>100_bases
TGTTTTGGTATACATTAAGACGTGTAAACTTATTCATTATTACACTTGGAATTCTGACTTTAGTGGGCTTTGCTCTACTT
CGATTAGACCAAACCTCACC

Product: peptide transport periplasmic protein SapA precursor

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKALIRLSLSICTLGFLAGCGESVTHEQIREKGFIYCGQGNPSTFNPQLVDSGITSESLSPQIFDTLLTLDPMTYRPKQN
LATSWSVDKSGTEYTFKLRPNVAFQTTDWFAPTRTMNAQDVVFSFERIIDSSNPFHYVGGGLYPWFAGIDFQNLIVEITA
VDDLTVKFQLSRPDNSFLNNIATSYAVIHSKEYANKLITTDEKNEIDSKPVGTGPFYLDEYQINDLVRLKKNKHYWKGEV
QMDQVVFDTSQRGTGTLAKLLRSECDVLNSPISSQIPIIQAHEELKISATPAVNVSFIALNTEHPALRDARVRKALNLAI
NRQNILDSVYYGTGTKAYTLLPPTSWAYQKDSVQVRYDRNYALALLREAGVEPGLELSMWVPLEPRAYNPSPRKTAELIQ
ANLADIGIELKLYTDDRFDRTQLSSISSTDLLLTGWIGSTGDPDNFLRPLLSCSSERAGLNVSMWCNSDFDFLLDLALEI
NQQRHRVNLYRQAQNILNEEVPVIPLAHGVQFRVHDRSLAGFKRSPFNAQPFDQVRREVN

Sequences:

>Translated_540_residues
MKALIRLSLSICTLGFLAGCGESVTHEQIREKGFIYCGQGNPSTFNPQLVDSGITSESLSPQIFDTLLTLDPMTYRPKQN
LATSWSVDKSGTEYTFKLRPNVAFQTTDWFAPTRTMNAQDVVFSFERIIDSSNPFHYVGGGLYPWFAGIDFQNLIVEITA
VDDLTVKFQLSRPDNSFLNNIATSYAVIHSKEYANKLITTDEKNEIDSKPVGTGPFYLDEYQINDLVRLKKNKHYWKGEV
QMDQVVFDTSQRGTGTLAKLLRSECDVLNSPISSQIPIIQAHEELKISATPAVNVSFIALNTEHPALRDARVRKALNLAI
NRQNILDSVYYGTGTKAYTLLPPTSWAYQKDSVQVRYDRNYALALLREAGVEPGLELSMWVPLEPRAYNPSPRKTAELIQ
ANLADIGIELKLYTDDRFDRTQLSSISSTDLLLTGWIGSTGDPDNFLRPLLSCSSERAGLNVSMWCNSDFDFLLDLALEI
NQQRHRVNLYRQAQNILNEEVPVIPLAHGVQFRVHDRSLAGFKRSPFNAQPFDQVRREVN
>Mature_540_residues
MKALIRLSLSICTLGFLAGCGESVTHEQIREKGFIYCGQGNPSTFNPQLVDSGITSESLSPQIFDTLLTLDPMTYRPKQN
LATSWSVDKSGTEYTFKLRPNVAFQTTDWFAPTRTMNAQDVVFSFERIIDSSNPFHYVGGGLYPWFAGIDFQNLIVEITA
VDDLTVKFQLSRPDNSFLNNIATSYAVIHSKEYANKLITTDEKNEIDSKPVGTGPFYLDEYQINDLVRLKKNKHYWKGEV
QMDQVVFDTSQRGTGTLAKLLRSECDVLNSPISSQIPIIQAHEELKISATPAVNVSFIALNTEHPALRDARVRKALNLAI
NRQNILDSVYYGTGTKAYTLLPPTSWAYQKDSVQVRYDRNYALALLREAGVEPGLELSMWVPLEPRAYNPSPRKTAELIQ
ANLADIGIELKLYTDDRFDRTQLSSISSTDLLLTGWIGSTGDPDNFLRPLLSCSSERAGLNVSMWCNSDFDFLLDLALEI
NQQRHRVNLYRQAQNILNEEVPVIPLAHGVQFRVHDRSLAGFKRSPFNAQPFDQVRREVN

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=537, Percent_Identity=41.8994413407821, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1789966, Length=536, Percent_Identity=35.0746268656716, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1787052, Length=524, Percent_Identity=27.6717557251908, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789397, Length=518, Percent_Identity=26.6409266409266, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787762, Length=533, Percent_Identity=22.5140712945591, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787495, Length=513, Percent_Identity=22.2222222222222, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081878, Length=469, Percent_Identity=24.9466950959488, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789887, Length=554, Percent_Identity=21.841155234657, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60853; Mature: 60853

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALIRLSLSICTLGFLAGCGESVTHEQIREKGFIYCGQGNPSTFNPQLVDSGITSESLS
CCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCCCCCCHHHCCCCCCCCCC
PQIFDTLLTLDPMTYRPKQNLATSWSVDKSGTEYTFKLRPNVAFQTTDWFAPTRTMNAQD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHH
VVFSFERIIDSSNPFHYVGGGLYPWFAGIDFQNLIVEITAVDDLTVKFQLSRPDNSFLNN
HHHHHHHHHCCCCCEEEECCCCCHHHCCCCHHHEEEEEEEECCEEEEEEECCCCHHHHHH
IATSYAVIHSKEYANKLITTDEKNEIDSKPVGTGPFYLDEYQINDLVRLKKNKHYWKGEV
HHHHHHEEECHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCEECCCE
QMDQVVFDTSQRGTGTLAKLLRSECDVLNSPISSQIPIIQAHEELKISATPAVNVSFIAL
EEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEECCCCEEEEEEE
NTEHPALRDARVRKALNLAINRQNILDSVYYGTGTKAYTLLPPTSWAYQKDSVQVRYDRN
ECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCC
YALALLREAGVEPGLELSMWVPLEPRAYNPSPRKTAELIQANLADIGIELKLYTDDRFDR
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCH
TQLSSISSTDLLLTGWIGSTGDPDNFLRPLLSCSSERAGLNVSMWCNSDFDFLLDLALEI
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
NQQRHRVNLYRQAQNILNEEVPVIPLAHGVQFRVHDRSLAGFKRSPFNAQPFDQVRREVN
HHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKALIRLSLSICTLGFLAGCGESVTHEQIREKGFIYCGQGNPSTFNPQLVDSGITSESLS
CCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCCCCCCHHHCCCCCCCCCC
PQIFDTLLTLDPMTYRPKQNLATSWSVDKSGTEYTFKLRPNVAFQTTDWFAPTRTMNAQD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHH
VVFSFERIIDSSNPFHYVGGGLYPWFAGIDFQNLIVEITAVDDLTVKFQLSRPDNSFLNN
HHHHHHHHHCCCCCEEEECCCCCHHHCCCCHHHEEEEEEEECCEEEEEEECCCCHHHHHH
IATSYAVIHSKEYANKLITTDEKNEIDSKPVGTGPFYLDEYQINDLVRLKKNKHYWKGEV
HHHHHHEEECHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCEECCCE
QMDQVVFDTSQRGTGTLAKLLRSECDVLNSPISSQIPIIQAHEELKISATPAVNVSFIAL
EEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEECCCCEEEEEEE
NTEHPALRDARVRKALNLAINRQNILDSVYYGTGTKAYTLLPPTSWAYQKDSVQVRYDRN
ECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCC
YALALLREAGVEPGLELSMWVPLEPRAYNPSPRKTAELIQANLADIGIELKLYTDDRFDR
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCH
TQLSSISSTDLLLTGWIGSTGDPDNFLRPLLSCSSERAGLNVSMWCNSDFDFLLDLALEI
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
NQQRHRVNLYRQAQNILNEEVPVIPLAHGVQFRVHDRSLAGFKRSPFNAQPFDQVRREVN
HHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]