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The map label for this gene is sapB [H]

Identifier: 218709975

GI number: 218709975

Start: 2141793

End: 2142755

Strand: Direct

Name: sapB [H]

Synonym: VS_1994

Alternate gene names: 218709975

Gene position: 2141793-2142755 (Clockwise)

Preceding gene: 218709974

Following gene: 218709976

Centisome position: 64.92

GC content: 45.07

Gene sequence:

>963_bases
ATGTTTTGGTATACATTAAGACGTGTAAACTTATTCATTATTACACTTGGAATTCTGACTTTAGTGGGCTTTGCTCTACT
TCGATTAGACCAAACCTCACCTTGGGCAATTCAAGATTTTTGGCCCGGCTGGCTTAGCTACGTCACCGAATTATCGACGC
TGAATTTCGGCCTGAGCAAACACGGTGTTCCTATCATCGAAGAGCTTGTCGTGGTATTCCCTGCGACGTTAGAATTGTGC
TTCTTTGCTTTTTTACTCGCACTGTTTATCGGCATCCCTTTCGGCACAATTGCCGGTATGAGACAAGGAAAATTTGTTGA
TACCACCATATCGTTTACTTCAATGGCAGGCTACTCAGCACCTATTTTTTGGGTCGCATTGCTAATGATCATGGTGTTCT
CACTGCACTTCGAGATGTTCCCTGTTGGGGGGCGATACGATCTACTTTACGAAATTGAACACGTGACTGGCTTTGCAATG
ATTGATGCCTTCTTTGCCGAAGGTAACTATCGAGCACACGCCCTACAGAGCGTAATAGACCATATGGCGTTACCTTGTCT
TGTTTTGGCGCTCGCTCCAACAACCCAGGTTATCCGACTAATGAGAGCGTCAGTGGCTGAAGTGATGGCTCAAAACTACA
TCCGTGCCGCACGAATCAAAGGTCTTTCAACAAGAGAGATCGTCACTCAACACGTATTAAGAAATGCAATACCACCGATT
ATTCCTAAAGTCGGCGTTCAACTCTCCAGTATGTTAACGCTCGCTATCATCACCGAGTCTATCTTTAACTGGCCGGGTAT
TGGCCGCTGGTTGCTGGATGCATTATCCAATCAAGATTACGTTTCAATTCAAGCTGGTGTAATTGTGGTAGCAACCTTGG
TTTTGACCGCGAATATACTGTCCGATCTACTGGGCGCGATGGTCAATCCTCTGGTAAGGAAGGAATGGTATGCTAACAAA
TAA

Upstream 100 bases:

>100_bases
ACGGCGTGCAATTCCGTGTGCACGATCGTTCGCTAGCAGGTTTTAAACGCAGTCCTTTTAATGCTCAACCTTTTGATCAA
GTCAGAAGGGAGGTGAACTG

Downstream 100 bases:

>100_bases
CGTCTACCAAGAAGAACAGATTCCAACTCAATTCGAGCGTTTCTGGCGCAGCTTCCGTGGCAATAATCTCGCAATGTTTG
GACTATGGTGTTTGGTCTTG

Product: peptide transport system permease SapB

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MFWYTLRRVNLFIITLGILTLVGFALLRLDQTSPWAIQDFWPGWLSYVTELSTLNFGLSKHGVPIIEELVVVFPATLELC
FFAFLLALFIGIPFGTIAGMRQGKFVDTTISFTSMAGYSAPIFWVALLMIMVFSLHFEMFPVGGRYDLLYEIEHVTGFAM
IDAFFAEGNYRAHALQSVIDHMALPCLVLALAPTTQVIRLMRASVAEVMAQNYIRAARIKGLSTREIVTQHVLRNAIPPI
IPKVGVQLSSMLTLAIITESIFNWPGIGRWLLDALSNQDYVSIQAGVIVVATLVLTANILSDLLGAMVNPLVRKEWYANK

Sequences:

>Translated_320_residues
MFWYTLRRVNLFIITLGILTLVGFALLRLDQTSPWAIQDFWPGWLSYVTELSTLNFGLSKHGVPIIEELVVVFPATLELC
FFAFLLALFIGIPFGTIAGMRQGKFVDTTISFTSMAGYSAPIFWVALLMIMVFSLHFEMFPVGGRYDLLYEIEHVTGFAM
IDAFFAEGNYRAHALQSVIDHMALPCLVLALAPTTQVIRLMRASVAEVMAQNYIRAARIKGLSTREIVTQHVLRNAIPPI
IPKVGVQLSSMLTLAIITESIFNWPGIGRWLLDALSNQDYVSIQAGVIVVATLVLTANILSDLLGAMVNPLVRKEWYANK
>Mature_320_residues
MFWYTLRRVNLFIITLGILTLVGFALLRLDQTSPWAIQDFWPGWLSYVTELSTLNFGLSKHGVPIIEELVVVFPATLELC
FFAFLLALFIGIPFGTIAGMRQGKFVDTTISFTSMAGYSAPIFWVALLMIMVFSLHFEMFPVGGRYDLLYEIEHVTGFAM
IDAFFAEGNYRAHALQSVIDHMALPCLVLALAPTTQVIRLMRASVAEVMAQNYIRAARIKGLSTREIVTQHVLRNAIPPI
IPKVGVQLSSMLTLAIITESIFNWPGIGRWLLDALSNQDYVSIQAGVIVVATLVLTANILSDLLGAMVNPLVRKEWYANK

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4168

COG function: function code V; ABC-type antimicrobial peptide transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787550, Length=316, Percent_Identity=50, Blast_Score=310, Evalue=7e-86,
Organism=Escherichia coli, GI1789965, Length=343, Percent_Identity=36.734693877551, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1787761, Length=247, Percent_Identity=37.6518218623482, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789888, Length=263, Percent_Identity=29.6577946768061, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787497, Length=334, Percent_Identity=29.0419161676647, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787053, Length=279, Percent_Identity=29.7491039426523, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788504, Length=250, Percent_Identity=25.6, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35693; Mature: 35693

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]