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The map label for this gene is pspF [H]

Identifier: 218709973

GI number: 218709973

Start: 2139046

End: 2140068

Strand: Direct

Name: pspF [H]

Synonym: VS_1992

Alternate gene names: 218709973

Gene position: 2139046-2140068 (Clockwise)

Preceding gene: 218709963

Following gene: 218709974

Centisome position: 64.83

GC content: 44.28

Gene sequence:

>1023_bases
ATGCAGCAGAATCTCATTGGTGAATCCCCTAGTTTTCTCTCTGTTTTGGATAAAGTGTCTCAACTCGCACCAATCGAAAG
ACCGATTCTGATCATAGGGGAGCGTGGTACAGGTAAAGAGTTAATCGCTCAAAGGCTGCATTACCTTTCAAGGCGCTGGG
ACCAGCCATTGATCTCACTGAACTGCTCAACCCTTAGCGAAGGGTTGATTGATTCAGAGTTGTTTGGTCATGAATCAGGT
TCTTTTACCGGTTCAAAAGGTCGTCATCAAGGTCGTTTTGAAAGGGCTGAAGGTGGAACTCTGTTCTTAGATGAACTAGC
GACCACCCCCCTTGCCGTTCAAGAGAAGCTATTGCGTGTTATTGAATACGGGCAATATGAGCGTGTTGGCGGACAAAAGG
CGCTAAGTGCCAATGTAAGACTGGTGTGTGCGACCAATGCTGACTTACCAGACATGGCGTTAAAAGGCGAATTCAGACCC
GATTTATTAGATAGACTGGCGTTTGATGTCATTACTATCCCACCTCTTCGCGAACGAAAAGAAGACATCACGCTGCTAGC
AGAATACTACGCCATTAAGATGTGTCGAGAGTTAGAATTGGAGCTGTTTGTCGGTTTTGCTCCATCTGCTGTTGAGGCAC
TATTGGATTATTCTTGGCCGGGAAATGTGAGAGAACTCAAAAACGTCGTTGAACGAGCTATCTACCAGCACGGTAAAGAT
CCATATCCTATTGAATCTTTGGTGTTTAACCCCTTTAAACCAACATGGCAAATAGATCAAGATTCCACGCACGAAACTGG
CAATGATCTGTCCAACAGCCATACTCCATTAGAGCACTCATTTTCTTTGCCGCTTGATTACAAAGCATGGCAAGAACAGC
AAGACATTAAATTACTCAATCAAGCTCTAAAACAGAGCAAGTATAACCAGAAACAAGCAGCAGAATTACTTGGGTTAACC
TACAACCAATTACGTGGAATGGTGAGGAAGTACGCGATTGTAGGACAAGCGAACGATAAATAG

Upstream 100 bases:

>100_bases
CACCACTACAGCTTTGTGACTAATGCTATTTACACCAAGTGATGTAAATAAAGCTCTCTATATCATGCCCATTGGAAATC
AGACCATACAAGGACCCATT

Downstream 100 bases:

>100_bases
CTGTCCACACTTGATTATTATTTGGAGCCGAATAAATAAAATGTTAAATTGTCGGGATCTTGAGGCTCCTCTAACGCCTC
GTTTCGAAAAGTATTTCTTT

Product: Psp operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MQQNLIGESPSFLSVLDKVSQLAPIERPILIIGERGTGKELIAQRLHYLSRRWDQPLISLNCSTLSEGLIDSELFGHESG
SFTGSKGRHQGRFERAEGGTLFLDELATTPLAVQEKLLRVIEYGQYERVGGQKALSANVRLVCATNADLPDMALKGEFRP
DLLDRLAFDVITIPPLRERKEDITLLAEYYAIKMCRELELELFVGFAPSAVEALLDYSWPGNVRELKNVVERAIYQHGKD
PYPIESLVFNPFKPTWQIDQDSTHETGNDLSNSHTPLEHSFSLPLDYKAWQEQQDIKLLNQALKQSKYNQKQAAELLGLT
YNQLRGMVRKYAIVGQANDK

Sequences:

>Translated_340_residues
MQQNLIGESPSFLSVLDKVSQLAPIERPILIIGERGTGKELIAQRLHYLSRRWDQPLISLNCSTLSEGLIDSELFGHESG
SFTGSKGRHQGRFERAEGGTLFLDELATTPLAVQEKLLRVIEYGQYERVGGQKALSANVRLVCATNADLPDMALKGEFRP
DLLDRLAFDVITIPPLRERKEDITLLAEYYAIKMCRELELELFVGFAPSAVEALLDYSWPGNVRELKNVVERAIYQHGKD
PYPIESLVFNPFKPTWQIDQDSTHETGNDLSNSHTPLEHSFSLPLDYKAWQEQQDIKLLNQALKQSKYNQKQAAELLGLT
YNQLRGMVRKYAIVGQANDK
>Mature_340_residues
MQQNLIGESPSFLSVLDKVSQLAPIERPILIIGERGTGKELIAQRLHYLSRRWDQPLISLNCSTLSEGLIDSELFGHESG
SFTGSKGRHQGRFERAEGGTLFLDELATTPLAVQEKLLRVIEYGQYERVGGQKALSANVRLVCATNADLPDMALKGEFRP
DLLDRLAFDVITIPPLRERKEDITLLAEYYAIKMCRELELELFVGFAPSAVEALLDYSWPGNVRELKNVVERAIYQHGKD
PYPIESLVFNPFKPTWQIDQDSTHETGNDLSNSHTPLEHSFSLPLDYKAWQEQQDIKLLNQALKQSKYNQKQAAELLGLT
YNQLRGMVRKYAIVGQANDK

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=61.7469879518072, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1788550, Length=330, Percent_Identity=36.0606060606061, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790437, Length=233, Percent_Identity=45.4935622317597, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=45.8874458874459, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=46.0869565217391, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=38.0664652567976, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=44.6351931330472, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=34.3283582089552, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=41.6309012875536, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1786524, Length=259, Percent_Identity=39.3822393822394, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=37.0689655172414, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789828, Length=270, Percent_Identity=30.3703703703704, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=31.8627450980392, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 38444; Mature: 38444

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNLIGESPSFLSVLDKVSQLAPIERPILIIGERGTGKELIAQRLHYLSRRWDQPLISL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
NCSTLSEGLIDSELFGHESGSFTGSKGRHQGRFERAEGGTLFLDELATTPLAVQEKLLRV
EHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHH
IEYGQYERVGGQKALSANVRLVCATNADLPDMALKGEFRPDLLDRLAFDVITIPPLRERK
HHCCCHHHCCCCCEECCCEEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCHHHHH
EDITLLAEYYAIKMCRELELELFVGFAPSAVEALLDYSWPGNVRELKNVVERAIYQHGKD
HHHHHHHHHHHHHHHHHHCHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PYPIESLVFNPFKPTWQIDQDSTHETGNDLSNSHTPLEHSFSLPLDYKAWQEQQDIKLLN
CCCHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
QALKQSKYNQKQAAELLGLTYNQLRGMVRKYAIVGQANDK
HHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQQNLIGESPSFLSVLDKVSQLAPIERPILIIGERGTGKELIAQRLHYLSRRWDQPLISL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
NCSTLSEGLIDSELFGHESGSFTGSKGRHQGRFERAEGGTLFLDELATTPLAVQEKLLRV
EHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHH
IEYGQYERVGGQKALSANVRLVCATNADLPDMALKGEFRPDLLDRLAFDVITIPPLRERK
HHCCCHHHCCCCCEECCCEEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCHHHHH
EDITLLAEYYAIKMCRELELELFVGFAPSAVEALLDYSWPGNVRELKNVVERAIYQHGKD
HHHHHHHHHHHHHHHHHHCHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PYPIESLVFNPFKPTWQIDQDSTHETGNDLSNSHTPLEHSFSLPLDYKAWQEQQDIKLLN
CCCHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
QALKQSKYNQKQAAELLGLTYNQLRGMVRKYAIVGQANDK
HHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]