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The map label for this gene is trpA

Identifier: 218709952

GI number: 218709952

Start: 2115153

End: 2115959

Strand: Direct

Name: trpA

Synonym: VS_1971

Alternate gene names: 218709952

Gene position: 2115153-2115959 (Clockwise)

Preceding gene: 218709951

Following gene: 218709955

Centisome position: 64.11

GC content: 45.85

Gene sequence:

>807_bases
ATGGATCGCTATCAATCACTATTCACTCGCTTAGCTGAAAAGAATCAAGGTGCATTTGTACCATTCGTAACGGTTGGCGA
TCCTAACCCTGAACAGTCTCTTAAGATCATGGAAACCTTGGTTGAAGCGGGTGCAGATGCACTTGAGCTTGGTATCCCAT
TCTCAGATCCGCTTGCCGATGGCCCAACAATTCAAGGCGCAAACATTCGTGCTTTAGATTCTAAAGTAACGCCAGATGTG
TGTTTTGATCTTATTGGTCAAATTCGCGCTAAATACCCAGAGCTACCAATCGGCCTACTGATGTACGCAAACTTGGTTTA
CGCACGTGGTATAGAAAATTTTTACGAACGCTGCGCAAAAGCAGGTATCGATTCAGTACTGATTGCTGATGTACCAACCA
ACGAGAGCGGCGAGTTTGTTGCTGCAGCTAAAAAGTTTGGTATTCACCCAATCTTTATTGCTCCACCAACAGCAAGTGAT
GAGACGCTTCAATCGGTTTCTGAGCTAGGTGGTGGTTACACTTACCTACTTTCTCGTGCTGGCGTAACGGGTGCAGAAAC
AAAAGCAAACATGCCTGTGACTGCCCTACTTGATCGTTTGAACAAGTTTGATGCGCCACCAGCACTGCTTGGTTTCGGTA
TCTCGGCTCCTGAACAAGTGAAAGAAGCAATTGTAGCTGGCGCTGCAGGTGCTATCTCGGGCTCAGCAGTTGTGAAAATC
ATTGAAAACAACGTTGAACAACCAGAAGCAATGCTTAAAGCACTTGCTGAGTTTGTTACGCCAATGAAAGCGGCAACGCA
GAAGTAA

Upstream 100 bases:

>100_bases
ACCCAGAGAAAGAACAGCTATTAGTCGTTAACTTATCTGGTCGCGGTGACAAAGACATTTTCTCTGTACACGACATTCTT
AAAGAGAAAGGAGCATTATA

Downstream 100 bases:

>100_bases
CGGCTAGTAATATCATTGGTCATTAAATAGGACCAGCGATTCAAATACTAAAAGGCTCCACAACTGTGGAGCCTTTTTTA
TTACTCGTCATTCACGCTAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDRYQSLFTRLAEKNQGAFVPFVTVGDPNPEQSLKIMETLVEAGADALELGIPFSDPLADGPTIQGANIRALDSKVTPDV
CFDLIGQIRAKYPELPIGLLMYANLVYARGIENFYERCAKAGIDSVLIADVPTNESGEFVAAAKKFGIHPIFIAPPTASD
ETLQSVSELGGGYTYLLSRAGVTGAETKANMPVTALLDRLNKFDAPPALLGFGISAPEQVKEAIVAGAAGAISGSAVVKI
IENNVEQPEAMLKALAEFVTPMKAATQK

Sequences:

>Translated_268_residues
MDRYQSLFTRLAEKNQGAFVPFVTVGDPNPEQSLKIMETLVEAGADALELGIPFSDPLADGPTIQGANIRALDSKVTPDV
CFDLIGQIRAKYPELPIGLLMYANLVYARGIENFYERCAKAGIDSVLIADVPTNESGEFVAAAKKFGIHPIFIAPPTASD
ETLQSVSELGGGYTYLLSRAGVTGAETKANMPVTALLDRLNKFDAPPALLGFGISAPEQVKEAIVAGAAGAISGSAVVKI
IENNVEQPEAMLKALAEFVTPMKAATQK
>Mature_268_residues
MDRYQSLFTRLAEKNQGAFVPFVTVGDPNPEQSLKIMETLVEAGADALELGIPFSDPLADGPTIQGANIRALDSKVTPDV
CFDLIGQIRAKYPELPIGLLMYANLVYARGIENFYERCAKAGIDSVLIADVPTNESGEFVAAAKKFGIHPIFIAPPTASD
ETLQSVSELGGGYTYLLSRAGVTGAETKANMPVTALLDRLNKFDAPPALLGFGISAPEQVKEAIVAGAAGAISGSAVVKI
IENNVEQPEAMLKALAEFVTPMKAATQK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=62.9213483146067, Blast_Score=332, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6321412, Length=241, Percent_Identity=32.3651452282158, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_VIBSL (B7VGU8)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417573.1
- GeneID:   7161527
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1971
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28372; Mature: 28372

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRYQSLFTRLAEKNQGAFVPFVTVGDPNPEQSLKIMETLVEAGADALELGIPFSDPLAD
CCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQGANIRALDSKVTPDVCFDLIGQIRAKYPELPIGLLMYANLVYARGIENFYERCAK
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGIDSVLIADVPTNESGEFVAAAKKFGIHPIFIAPPTASDETLQSVSELGGGYTYLLSRA
CCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGAETKANMPVTALLDRLNKFDAPPALLGFGISAPEQVKEAIVAGAAGAISGSAVVKI
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
IENNVEQPEAMLKALAEFVTPMKAATQK
HHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRYQSLFTRLAEKNQGAFVPFVTVGDPNPEQSLKIMETLVEAGADALELGIPFSDPLAD
CCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQGANIRALDSKVTPDVCFDLIGQIRAKYPELPIGLLMYANLVYARGIENFYERCAK
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGIDSVLIADVPTNESGEFVAAAKKFGIHPIFIAPPTASDETLQSVSELGGGYTYLLSRA
CCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGAETKANMPVTALLDRLNKFDAPPALLGFGISAPEQVKEAIVAGAAGAISGSAVVKI
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
IENNVEQPEAMLKALAEFVTPMKAATQK
HHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA