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The map label for this gene is trpB1 [H]

Identifier: 218709951

GI number: 218709951

Start: 2113963

End: 2115153

Strand: Direct

Name: trpB1 [H]

Synonym: VS_1970

Alternate gene names: 218709951

Gene position: 2113963-2115153 (Clockwise)

Preceding gene: 218709950

Following gene: 218709952

Centisome position: 64.07

GC content: 47.61

Gene sequence:

>1191_bases
ATGGCTAAACTCGATGCCTACTTCGGTGAATACGGTGGTCAATACGTACCGCAGATCCTAGTGCCAGCACTAGACCAACT
AGAACAAGCATTTATCGATGCACAAGCCGATCCTGAGTTCCGCAGTGAATTCATGACGCTTCTGCAAGAGTACGCCGGTC
GTCCAACGGCACTAACGCTAACGCGTAACCTGACTAAAGGTACAAAAACCAAACTGTACCTAAAGCGTGAAGATCTACTT
CACGGCGGTGCTCACAAGACAAACCAAGTTCTTGGTCAAGCATTGCTAGCAAAACGTATGGGTAAACAGGAAATCATCGC
TGAAACTGGCGCAGGCCAACATGGTGTTGCAACGGCTCTAGCTTGTGCTCTATTGGGCCTTAAGTGTCGTGTTTACATGG
GTGCAAAAGACGTTGAACGTCAAAGCCCGAACGTGTTCCGTATGAAGCTAATGGGCGCAGAGGTTATCCCTGTTCATTCT
GGCTCTTCAACGCTAAAAGATGCATGCAACGAAGCACTACGTGACTGGTCTGCAACTTATGAAGACGCACACTACCTGCT
AGGTACTGCGGCTGGCCCTCACCCATTCCCAACGATTGTTCGTGATTTCCAACGCATGATCGGTGAAGAAACGAAGAACC
AAATTCTAGCTCGTGAAGGTCGCCTTCCTGATGCCGTTATCGCTTGTGTAGGTGGTGGTTCTAACGCTATCGGCATGTTC
GCTGATTTCATTGAAGAAGAGTCTGTTCGCCTAATCGGTGTAGAGCCTGCTGGTAAAGGTATTGATACCGACCAACACGG
CGCACCACTTAAACACGGTAAAACAGGTATTTTCTTCGGTATGAAAGCACCACTGATGCAAGATGAAAACGGCCAAGTAG
AAGAGTCTTACTCTGTTTCTGCGGGTCTAGATTTCCCATCAGTTGGTCCTCAACACGCGCACCTAAATGCGATTGGCCGT
GCTGAATACGACAACGTGACCGATGACGAAGCGCTAGAAGCGTTCCAATTGATTGCACGTAAAGAAGGTATTATCGCAGC
GCTAGAGTCATCTCATGCTTTGGCTCATGCTGTTAAAATGGCTCATGACGACCCAGAGAAAGAACAGCTATTAGTCGTTA
ACTTATCTGGTCGCGGTGACAAAGACATTTTCTCTGTACACGACATTCTTAAAGAGAAAGGAGCATTATAA

Upstream 100 bases:

>100_bases
AAACTAAAAACATATTTGTGGGCTTTGCCTTTTGCGAAGCTCAAGCAAGATTAAATGACTTGGTGTGAAGACACATCAAT
AGAATTGAAGGAATAACACA

Downstream 100 bases:

>100_bases
TGGATCGCTATCAATCACTATTCACTCGCTTAGCTGAAAAGAATCAAGGTGCATTTGTACCATTCGTAACGGTTGGCGAT
CCTAACCCTGAACAGTCTCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADPEFRSEFMTLLQEYAGRPTALTLTRNLTKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKQEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHS
GSSTLKDACNEALRDWSATYEDAHYLLGTAAGPHPFPTIVRDFQRMIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
ADFIEEESVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDENGQVEESYSVSAGLDFPSVGPQHAHLNAIGR
AEYDNVTDDEALEAFQLIARKEGIIAALESSHALAHAVKMAHDDPEKEQLLVVNLSGRGDKDIFSVHDILKEKGAL

Sequences:

>Translated_396_residues
MAKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADPEFRSEFMTLLQEYAGRPTALTLTRNLTKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKQEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHS
GSSTLKDACNEALRDWSATYEDAHYLLGTAAGPHPFPTIVRDFQRMIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
ADFIEEESVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDENGQVEESYSVSAGLDFPSVGPQHAHLNAIGR
AEYDNVTDDEALEAFQLIARKEGIIAALESSHALAHAVKMAHDDPEKEQLLVVNLSGRGDKDIFSVHDILKEKGAL
>Mature_395_residues
AKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADPEFRSEFMTLLQEYAGRPTALTLTRNLTKGTKTKLYLKREDLLH
GGAHKTNQVLGQALLAKRMGKQEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSG
SSTLKDACNEALRDWSATYEDAHYLLGTAAGPHPFPTIVRDFQRMIGEETKNQILAREGRLPDAVIACVGGGSNAIGMFA
DFIEEESVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDENGQVEESYSVSAGLDFPSVGPQHAHLNAIGRA
EYDNVTDDEALEAFQLIARKEGIIAALESSHALAHAVKMAHDDPEKEQLLVVNLSGRGDKDIFSVHDILKEKGAL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=393, Percent_Identity=79.6437659033079, Blast_Score=665, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=52.4421593830334, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 42903; Mature: 42772

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADPEFRSEFMTLLQEYAGRPTALTL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
TRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKQEIIAETGAGQHGVATAL
ECCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSGSSTLKDACNEALRDWSATY
HHHHHHHHEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCH
EDAHYLLGTAAGPHPFPTIVRDFQRMIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
HCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
ADFIEEESVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDENGQVEESYSVS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
AGLDFPSVGPQHAHLNAIGRAEYDNVTDDEALEAFQLIARKEGIIAALESSHALAHAVKM
CCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
AHDDPEKEQLLVVNLSGRGDKDIFSVHDILKEKGAL
HCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADPEFRSEFMTLLQEYAGRPTALTL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
TRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKQEIIAETGAGQHGVATAL
ECCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSGSSTLKDACNEALRDWSATY
HHHHHHHHEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCH
EDAHYLLGTAAGPHPFPTIVRDFQRMIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
HCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
ADFIEEESVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDENGQVEESYSVS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
AGLDFPSVGPQHAHLNAIGRAEYDNVTDDEALEAFQLIARKEGIIAALESSHALAHAVKM
CCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
AHDDPEKEQLLVVNLSGRGDKDIFSVHDILKEKGAL
HCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]