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The map label for this gene is rsuA [H]

Identifier: 218709840

GI number: 218709840

Start: 2002548

End: 2003291

Strand: Reverse

Name: rsuA [H]

Synonym: VS_1853

Alternate gene names: 218709840

Gene position: 2003291-2002548 (Counterclockwise)

Preceding gene: 218709842

Following gene: 218709839

Centisome position: 60.72

GC content: 43.95

Gene sequence:

>744_bases
TTGAGCAAAGGTGACTATAATACCCGACTTAGATTAGTTGAGGTATTCATGCGTTTAGATAAATTTCTGTGCGATGCGTT
AGGCGTCACTCGAAGAGAAGCAACACACTTATTAAAATCAAAAGCAGTGACAGTAAATGATGTCATTCAAAAAAGCGGCT
CACTTAAGGTAACTGAAGAGTGCGTTGTGGAATGGCAAGGCAATGAACTGAATGTTCACGGCCCGCGTTACATCATGCTT
TACAAGCCAGAAGGCTTTGTTTGTTCGCATGAAGATGGTTCAAATCGCACTGCGTTTGAATTACTCGATGAAATCAAAAT
GGACAAGCTGCACTTTGCAGGTCGTTTAGATGTTGATACAACGGGTCTTGTCTTAATGACAGACGACGGTAAGTGGTCTC
ACCGCATCACATCGCCAAAGCACAAGTGCGAGAAGATGTACCGAGTGTGGTTGGTTGAACCTGTTGAAGACGATTACGTT
GAAAAGTTCAAAGAGGGCATCCAGCTTAAGAGCGAAGACGGCCTAACACTTCCAGCACACCTTGAAGTACGTGCAGAGCG
TGAAGTGTTGCTAACGATTCACGAAGGAAAATACCACCAAGTAAAACGCATGTTTGCAGCACTTGGTAACAAGGTCGAAG
CACTGCACCGTGAACGTATTGGTGAAATCGAGATGGACGAGTCTTTAGAGTTGGGTGAATACCGTTACCTAACACAAGAA
GAAGTAGACTCTATCTGGAAGTAA

Upstream 100 bases:

>100_bases
ATGAATCACTGATTTTACTGAGTCAGCTTGGTACGGGCGGAGTGTATACATATCGTGTCTTGTAAAATGAAAAACAAATA
GTGTGAAATAGCTCAACTAA

Downstream 100 bases:

>100_bases
TCTTTTCTACCGTAGATGTGGCCGACCAAGGATAGCGTCGGCTGATTATTTTGACTGGTACTAGGACAGTTTCAGCCATT
ATTACTATATGTTCATGTAT

Product: 16S rRNA uridine-516 pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSKGDYNTRLRLVEVFMRLDKFLCDALGVTRREATHLLKSKAVTVNDVIQKSGSLKVTEECVVEWQGNELNVHGPRYIML
YKPEGFVCSHEDGSNRTAFELLDEIKMDKLHFAGRLDVDTTGLVLMTDDGKWSHRITSPKHKCEKMYRVWLVEPVEDDYV
EKFKEGIQLKSEDGLTLPAHLEVRAEREVLLTIHEGKYHQVKRMFAALGNKVEALHRERIGEIEMDESLELGEYRYLTQE
EVDSIWK

Sequences:

>Translated_247_residues
MSKGDYNTRLRLVEVFMRLDKFLCDALGVTRREATHLLKSKAVTVNDVIQKSGSLKVTEECVVEWQGNELNVHGPRYIML
YKPEGFVCSHEDGSNRTAFELLDEIKMDKLHFAGRLDVDTTGLVLMTDDGKWSHRITSPKHKCEKMYRVWLVEPVEDDYV
EKFKEGIQLKSEDGLTLPAHLEVRAEREVLLTIHEGKYHQVKRMFAALGNKVEALHRERIGEIEMDESLELGEYRYLTQE
EVDSIWK
>Mature_246_residues
SKGDYNTRLRLVEVFMRLDKFLCDALGVTRREATHLLKSKAVTVNDVIQKSGSLKVTEECVVEWQGNELNVHGPRYIMLY
KPEGFVCSHEDGSNRTAFELLDEIKMDKLHFAGRLDVDTTGLVLMTDDGKWSHRITSPKHKCEKMYRVWLVEPVEDDYVE
KFKEGIQLKSEDGLTLPAHLEVRAEREVLLTIHEGKYHQVKRMFAALGNKVEALHRERIGEIEMDESLELGEYRYLTQEE
VDSIWK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=56.0869565217391, Blast_Score=251, Evalue=3e-68,
Organism=Escherichia coli, GI1787524, Length=228, Percent_Identity=29.8245614035088, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=25.5144032921811, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=30.2197802197802, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28655; Mature: 28524

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGDYNTRLRLVEVFMRLDKFLCDALGVTRREATHLLKSKAVTVNDVIQKSGSLKVTEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCEEEEHH
CVVEWQGNELNVHGPRYIMLYKPEGFVCSHEDGSNRTAFELLDEIKMDKLHFAGRLDVDT
HEEEECCCEEEECCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
TGLVLMTDDGKWSHRITSPKHKCEKMYRVWLVEPVEDDYVEKFKEGIQLKSEDGLTLPAH
CEEEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHCCEEECCCCCCCCEE
LEVRAEREVLLTIHEGKYHQVKRMFAALGNKVEALHRERIGEIEMDESLELGEYRYLTQE
EEEEECCEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHH
EVDSIWK
HHHHHCC
>Mature Secondary Structure 
SKGDYNTRLRLVEVFMRLDKFLCDALGVTRREATHLLKSKAVTVNDVIQKSGSLKVTEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCEEEEHH
CVVEWQGNELNVHGPRYIMLYKPEGFVCSHEDGSNRTAFELLDEIKMDKLHFAGRLDVDT
HEEEECCCEEEECCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
TGLVLMTDDGKWSHRITSPKHKCEKMYRVWLVEPVEDDYVEKFKEGIQLKSEDGLTLPAH
CEEEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHCCEEECCCCCCCCEE
LEVRAEREVLLTIHEGKYHQVKRMFAALGNKVEALHRERIGEIEMDESLELGEYRYLTQE
EEEEECCEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHH
EVDSIWK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA