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The map label for this gene is bcr [H]

Identifier: 218709839

GI number: 218709839

Start: 2001096

End: 2002313

Strand: Reverse

Name: bcr [H]

Synonym: VS_1852

Alternate gene names: 218709839

Gene position: 2002313-2001096 (Counterclockwise)

Preceding gene: 218709840

Following gene: 218709838

Centisome position: 60.69

GC content: 47.54

Gene sequence:

>1218_bases
ATGCAAACGTCTACCTCACAGACCCCAAGCTCACAACCACAAACGCCTCAGTTAGGTTGGATGCTATTTTTGGTTTTGGG
CGCTATTGGTGCTTTGACACCACTCGCCATCGATATGTACCTACCTGCAATGCCAACGATCGCCAAAGATCTTGGTGTGA
CAGCAGGGGAAGTGCAAATCACACTTACCGCGTACACCGCAGGTTTCGCTTTAGGTCAGTTGTTACATGGTCCGTTAGCC
GACAGTTATGGTCGCAAGCCAGTTCTACTGATTGGTGTGCTCTTCTTTGCGATAGCGTCTGTCGTGAGTGCAACCACTCA
TGGCATTGAAGCGTTAACGTTAGTTCGTACCGCTCAAGGTTTTGCAGGTGCGGCGGCGGCGGTCATTATCCAAGCGGTTG
TGCGTGATATGTTCGACCGTGAAGATTTCGCAAGAACCATGTCGTTCGTTACCTTAGTGATGACGGTTGCGCCACTTATC
GCGCCGATGATTGGCGGCTATTTGGCATTGTGGTTCGGCTGGCGTTCAATCTTTTGGGTGTTAGCTATTTTTGCAGTGAT
TGTGATTCTCGCGGTGATAATCAAAATCCCTGAAACTCTGCCTGTTGAAAATCGTCAACCATTACGCTTTAAAACCACGA
TTCGTAATTACGCTCGTTTATGTAAAAACTCGACCGCTATGGGCCTAATTTTCTCGGGTGCGTTCTCGTTCTCTGGGATG
TTTGCATTCTTAACCGCAGGCTCTTTTGTCTACATTGATGTCTATGGAGTACGTCCTGACCTGTTTGGTTACCTGTTTGG
TCTGAACATCGTTGCGATGATTCTAATGACGACAATTAATGGTCGAATTGTTAAGAAGGTTGGCTCTCATACCATGCTGA
GAGCCGCGCTGGTCATTCAATTATTAGCTGGCGCCGGTTTACTGGTTGGTTGGTTATTGGATCTCGGGCTTTGGGGTATT
GTGCCGTTTGTGATGCTATTTATTGGCACCATTTCTACTATCGGCAGTAACTCTATGGGTCTATTGCTCAGTGGTTATCC
AAACATGGCCGGCACGGCTTCATCGCTTGCGGGAACATTAAGATTTGGTACTGGTTCTGTCGTTGGAGCTATCGTTGCGA
TGCTGCCAAGTGACAGTGCTGGGTCTATGGCTATGGTAATGGCTGCGTGTGCAGTAATGTCAGCATTACTATATTGGACA
TTAGGAAAGAAGGCATAA

Upstream 100 bases:

>100_bases
GTGTATCACTGTGCGTATTCGTGTATTGCTATGTGTATATTCGTATATTGCTGCGTGCATGCTCGTGTTCACATAGAACT
TAGTACTTAGTCGGAGAGTT

Downstream 100 bases:

>100_bases
TGTCAAAATACTATATTGAAATTCAGAAGTTAGTGAATGATGCGTTAGGCGAGCTTTACGCTCTGCACAAAGCAGGCAAA
GCGATTGATGCGCCTATTGC

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MQTSTSQTPSSQPQTPQLGWMLFLVLGAIGALTPLAIDMYLPAMPTIAKDLGVTAGEVQITLTAYTAGFALGQLLHGPLA
DSYGRKPVLLIGVLFFAIASVVSATTHGIEALTLVRTAQGFAGAAAAVIIQAVVRDMFDREDFARTMSFVTLVMTVAPLI
APMIGGYLALWFGWRSIFWVLAIFAVIVILAVIIKIPETLPVENRQPLRFKTTIRNYARLCKNSTAMGLIFSGAFSFSGM
FAFLTAGSFVYIDVYGVRPDLFGYLFGLNIVAMILMTTINGRIVKKVGSHTMLRAALVIQLLAGAGLLVGWLLDLGLWGI
VPFVMLFIGTISTIGSNSMGLLLSGYPNMAGTASSLAGTLRFGTGSVVGAIVAMLPSDSAGSMAMVMAACAVMSALLYWT
LGKKA

Sequences:

>Translated_405_residues
MQTSTSQTPSSQPQTPQLGWMLFLVLGAIGALTPLAIDMYLPAMPTIAKDLGVTAGEVQITLTAYTAGFALGQLLHGPLA
DSYGRKPVLLIGVLFFAIASVVSATTHGIEALTLVRTAQGFAGAAAAVIIQAVVRDMFDREDFARTMSFVTLVMTVAPLI
APMIGGYLALWFGWRSIFWVLAIFAVIVILAVIIKIPETLPVENRQPLRFKTTIRNYARLCKNSTAMGLIFSGAFSFSGM
FAFLTAGSFVYIDVYGVRPDLFGYLFGLNIVAMILMTTINGRIVKKVGSHTMLRAALVIQLLAGAGLLVGWLLDLGLWGI
VPFVMLFIGTISTIGSNSMGLLLSGYPNMAGTASSLAGTLRFGTGSVVGAIVAMLPSDSAGSMAMVMAACAVMSALLYWT
LGKKA
>Mature_405_residues
MQTSTSQTPSSQPQTPQLGWMLFLVLGAIGALTPLAIDMYLPAMPTIAKDLGVTAGEVQITLTAYTAGFALGQLLHGPLA
DSYGRKPVLLIGVLFFAIASVVSATTHGIEALTLVRTAQGFAGAAAAVIIQAVVRDMFDREDFARTMSFVTLVMTVAPLI
APMIGGYLALWFGWRSIFWVLAIFAVIVILAVIIKIPETLPVENRQPLRFKTTIRNYARLCKNSTAMGLIFSGAFSFSGM
FAFLTAGSFVYIDVYGVRPDLFGYLFGLNIVAMILMTTINGRIVKKVGSHTMLRAALVIQLLAGAGLLVGWLLDLGLWGI
VPFVMLFIGTISTIGSNSMGLLLSGYPNMAGTASSLAGTLRFGTGSVVGAIVAMLPSDSAGSMAMVMAACAVMSALLYWT
LGKKA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=383, Percent_Identity=47.2584856396867, Blast_Score=346, Evalue=2e-96,
Organism=Escherichia coli, GI87082312, Length=361, Percent_Identity=28.808864265928, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI48994889, Length=361, Percent_Identity=31.0249307479224, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790146, Length=170, Percent_Identity=33.5294117647059, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790794, Length=288, Percent_Identity=24.6527777777778, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787065, Length=186, Percent_Identity=28.494623655914, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI2367379, Length=172, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787880, Length=337, Percent_Identity=24.9258160237389, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081738, Length=183, Percent_Identity=30.0546448087432, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=26.8115942028986, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323000, Length=157, Percent_Identity=29.9363057324841, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=170, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=180, Percent_Identity=25.5555555555556, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319480, Length=197, Percent_Identity=26.9035532994924, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42875; Mature: 42875

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]