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The map label for this gene is gcvA [H]

Identifier: 218709144

GI number: 218709144

Start: 1218446

End: 1219333

Strand: Reverse

Name: gcvA [H]

Synonym: VS_1151

Alternate gene names: 218709144

Gene position: 1219333-1218446 (Counterclockwise)

Preceding gene: 218709147

Following gene: 218709143

Centisome position: 36.96

GC content: 47.64

Gene sequence:

>888_bases
ATGAATCGCACACTTCCTTCAACCAAAACCTTGCTCGCGTTTTTGTCTACGGCAAGGCATCTCAACTTTACGCGCGCGGC
ACATGAGCTCAATGTTACGCAAGGTGCAGTGAGTCGTCAGGTATTGTCGTTTGAAGAAAGTCTTGGCTGCGATCTTTTCT
ACCGACATGCTCGCGGGTTGTCATTAACACCTAAGGGAGAAGAACTGGTTCCGCTGATTCAAGATACCATCCAACAACTG
CAATCGGCGTTAAATCAAATTGCCAGCTCTCCATCGAAGATCAAGCTCAACGCACCAAGTTGTATCACCTCTTGGCTACT
GCCTAAGTTGATGTCTTTTCAGCAAGCCTATCCTGAAATTGATGTCGAACTCACGTCGACCATCAAGCACGTCTTCGAAC
CAAGCTTTGACCCGTTTGACGCCGTAATTACCTATGGTAAGAAGCCAAACCAGCAATCGATTATCAGCCAGTTATTGTTT
AACGAGCAGCTAGCGCCCGTTTGCCAAGCGCAAAGCATTACGCAAAGCCATCTTGTCAACGACGCGTCGACCATCATCAA
GCCACATCAGCTCGCCCAATACACTTGGTTGCACGCCAACAATGAGCAGAGTGATTGGCGGCTTTGGTTGGAACACATAG
GCAGCCACGACCTTTCAAGCAAGAACAACCAACAATTCGCGACGCTCGATCAAGCGATGAACGCAGCCATTCAAGGGTTT
GGTATTGCCATAGGCGACATCACACTGGCCAAGCAAGATATTGATTTGGGTAGATTGGTGAAAGTGAGTGAAGGCAGTGT
CTTCTCTGGAAATGGTTACTTCTTACTGCAACCCAAGAATCGACAAAATACATCTTTATCTACGCTGGTTGATTGGCTCG
TCGATTAG

Upstream 100 bases:

>100_bases
CACAAATGATGATTAAGTATTTACATTCAAACAGAACACCATTGATAAATGAATCGAAATAATCACATAATAGCCATGAT
AAAAACTCATAGGTAAAGAT

Downstream 100 bases:

>100_bases
CGGCAATCATCTTGGGTAAGCCCCCACTGTAGCTTTAATAGATCGTCATAATGAGAATTTTACCCTTCTCAAAATGAAAC
TATTTTCCGGTAGCTACGTA

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNRTLPSTKTLLAFLSTARHLNFTRAAHELNVTQGAVSRQVLSFEESLGCDLFYRHARGLSLTPKGEELVPLIQDTIQQL
QSALNQIASSPSKIKLNAPSCITSWLLPKLMSFQQAYPEIDVELTSTIKHVFEPSFDPFDAVITYGKKPNQQSIISQLLF
NEQLAPVCQAQSITQSHLVNDASTIIKPHQLAQYTWLHANNEQSDWRLWLEHIGSHDLSSKNNQQFATLDQAMNAAIQGF
GIAIGDITLAKQDIDLGRLVKVSEGSVFSGNGYFLLQPKNRQNTSLSTLVDWLVD

Sequences:

>Translated_295_residues
MNRTLPSTKTLLAFLSTARHLNFTRAAHELNVTQGAVSRQVLSFEESLGCDLFYRHARGLSLTPKGEELVPLIQDTIQQL
QSALNQIASSPSKIKLNAPSCITSWLLPKLMSFQQAYPEIDVELTSTIKHVFEPSFDPFDAVITYGKKPNQQSIISQLLF
NEQLAPVCQAQSITQSHLVNDASTIIKPHQLAQYTWLHANNEQSDWRLWLEHIGSHDLSSKNNQQFATLDQAMNAAIQGF
GIAIGDITLAKQDIDLGRLVKVSEGSVFSGNGYFLLQPKNRQNTSLSTLVDWLVD
>Mature_295_residues
MNRTLPSTKTLLAFLSTARHLNFTRAAHELNVTQGAVSRQVLSFEESLGCDLFYRHARGLSLTPKGEELVPLIQDTIQQL
QSALNQIASSPSKIKLNAPSCITSWLLPKLMSFQQAYPEIDVELTSTIKHVFEPSFDPFDAVITYGKKPNQQSIISQLLF
NEQLAPVCQAQSITQSHLVNDASTIIKPHQLAQYTWLHANNEQSDWRLWLEHIGSHDLSSKNNQQFATLDQAMNAAIQGF
GIAIGDITLAKQDIDLGRLVKVSEGSVFSGNGYFLLQPKNRQNTSLSTLVDWLVD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=279, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=29.9319727891156, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=26.8965517241379, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=27.0547945205479, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=26.578073089701, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=270, Percent_Identity=25.5555555555556, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=194, Percent_Identity=26.2886597938144, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994962, Length=176, Percent_Identity=29.5454545454545, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=31.9018404907975, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790783, Length=264, Percent_Identity=26.8939393939394, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790262, Length=120, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32858; Mature: 32858

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTLPSTKTLLAFLSTARHLNFTRAAHELNVTQGAVSRQVLSFEESLGCDLFYRHARGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCCC
SLTPKGEELVPLIQDTIQQLQSALNQIASSPSKIKLNAPSCITSWLLPKLMSFQQAYPEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
DVELTSTIKHVFEPSFDPFDAVITYGKKPNQQSIISQLLFNEQLAPVCQAQSITQSHLVN
CHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DASTIIKPHQLAQYTWLHANNEQSDWRLWLEHIGSHDLSSKNNQQFATLDQAMNAAIQGF
CHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
GIAIGDITLAKQDIDLGRLVKVSEGSVFSGNGYFLLQPKNRQNTSLSTLVDWLVD
CEEECCEEEECCCCCCCCEEEECCCCEECCCCEEEEECCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNRTLPSTKTLLAFLSTARHLNFTRAAHELNVTQGAVSRQVLSFEESLGCDLFYRHARGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCCC
SLTPKGEELVPLIQDTIQQLQSALNQIASSPSKIKLNAPSCITSWLLPKLMSFQQAYPEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
DVELTSTIKHVFEPSFDPFDAVITYGKKPNQQSIISQLLFNEQLAPVCQAQSITQSHLVN
CHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DASTIIKPHQLAQYTWLHANNEQSDWRLWLEHIGSHDLSSKNNQQFATLDQAMNAAIQGF
CHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
GIAIGDITLAKQDIDLGRLVKVSEGSVFSGNGYFLLQPKNRQNTSLSTLVDWLVD
CEEECCEEEECCCCCCCCEEEECCCCEECCCCEEEEECCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]