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The map label for this gene is topA [H]

Identifier: 218709075

GI number: 218709075

Start: 1137164

End: 1139794

Strand: Direct

Name: topA [H]

Synonym: VS_1081

Alternate gene names: 218709075

Gene position: 1137164-1139794 (Clockwise)

Preceding gene: 218709073

Following gene: 218709076

Centisome position: 34.47

GC content: 45.04

Gene sequence:

>2631_bases
ATGGGTAAATCGCTCGTTATAGTGGAGTCGCCAGCCAAGGCTAAAACTATCAATAAATATCTTGGCAAAGACTTTGTCGT
TAAGTCGAGTGTAGGTCACGTTCGTGATTTACCAACGGCGGGTCAAAGTACAGGTCAAAAGGCTGCTGCAGTTTCAACCA
AGGGTATGAGCCCAGAAGATAAAGCTCGTATCAAGAAAGAAAAAGATCGCAAAGCGCTTATTAAAAAGATGGGTATCAAC
CCTTATCAAGAGTGGGAAGCGAATTACCAAATCTTACCGGGCAAAGAAAAAGTCGTTGCTGAATTGCAAAAACTGGCTGA
GAACGCAGACCATGTTTATCTCGCAACCGATTTGGACCGCGAAGGAGAAGCTATCGCATGGCACCTTCGTGAGATCATCG
GTGGTGATGAAACGCGATACAAACGAGTGGTTTTTAACGAAATCACTAAAAATGCTATTCAGCAAGCTTTCGAAACTCCA
GGCGAGCTAAATATCGACGGCGTAAACGCACAACAAGCACGACGTTTTATGGACCGTGTTGTTGGCTTTATGGTGTCACC
ACTGCTTTGGAAAAAAGTAGCACGAGGCCTATCCGCTGGCCGTGTTCAATCTGTAGCTGTAAAACTACTGGTTGAACGTG
AGCGCGAGATCAACGCTTTTATCCCTGAAGAGTTCTGGGATGTGCATGCTGATACCAAAACGGCAGAGAAAACAGATTTC
AGACTGCAAGTCGCTCAAAAAGACGGTGTTGCGTTTAAACCTGATAATGAAGCGCAAACACAAGCTGCAGTAAGCGTTTT
AGAAAAAGCGCAATACGAAGTATGTAAACGTGAAGACCGCCCAACTAAGAGTAAGCCGTCTGCACCTTACATCACGTCGA
CTCTGCAACAAGCGGCGAGCACACGTCTTGGTTACGGCGTAAAGAAAACCATGATGTTGGCTCAACGTTTGTATGAAGCG
GGTTACATCACTTATATGCGTACTGACTCGACCAACTTGAGTTCAGAAGCTGTAGAAGCCGCACGTGAATACATTGGTTC
TGAGTTTGGTGCACAATATCTCCCACCGAAAGCGCTTGTATACGGCAGCAAAGAGGGCGCACAAGAGGCTCACGAAGCGA
TTCGTCCTTCGAGTGTCGATGTTAAGTCTGAAGACCTAAACGGCGTAGACGCAGACGCACACAAGCTTTACTCGCTGATT
TGGAATCAATTTGTTGCATGTCAAATGACACCTGCGCAATACGATTCAACGACGGTAAGCGTTAAAGCTGATGAGTACAC
GTTAAAAGCGAAAGGCCGTATTCTTAAGTTTGATGGTTGGACTCGCGTTCAACGTCCAATGGGTAAAAATGAAGATACGA
TTCTTCCTGCTGTACAGGTTGGTGAAAAGCTAGACCTAATCGCATTAGACCCTAAACAGCATTTCACTAAGCCGCCAGCC
CGTTTTACTGAAGCGGCGCTAGTTAAAGAGCTTGAGAAGCGTGGTATTGGTCGTCCTTCAACGTACGCATCAATCATCTC
TACGATTCAAGACCGTGGTTATGTAAAAGTTGAACAGCGTCGTTTCTATGCTGAGAAGATGGGTGAAATTGTTACTGACC
GTCTAGATGGCAGTTTCAATGACCTAATGAACTATGACTTTACGGCTCGTATGGAGCAGAAGTTAGACCAAATTGCAGAA
GGCGAAGCAAGCTGGAAAGGCGTGCTAGATAACTTCTTCGAAGACTTTACAGGCGATTTGGAAAAAGCCGATTTAGATGA
AGACGCTGGTGGCATGAAGCCAAATCATATCGTTGAAACCGATATTGAGTGTCCAACTTGTAGCCGTAAAATGGGTATTC
GTACAGCTTCGACTGGCGTATTCCTAGGTTGTTCTGGTTATGCACTTCCACCAAAAGAGCGCTGTAAGACAACCATTAAC
CTTGGTGATGAAGACGGTATTATCAACGTTCTTGAAGAAGACGTTGAAACCGCAGCACTTCGAGCGAAAAAGCGTTGTCC
GATTTGTGAAACGGCAATGGATGCTTATCTGATCGATGATAAGCGTAAGATGCATGTGTGTGGTAACAACCCTAACTGTG
AAGGTTATGTGGTTGAACACGGCGAATTCAAAGTGAAAGGCTATGATGGTCCGCTTGTTGAGTGTGACAAATGTAGTTCT
GACATGGTTTTGAAGAATGGTCGTTTCGGTAAATACATGGACTGTACGAGTGAAGATTGTAAGAACACTCGTAAGATTCT
GAAGAATGGCGAAGTAGCGCCACCTAAAGAAGACCCAGTGCATTTCCCTGAACTCCCATGTGAAAATTCAGATGCATACT
TCGTACTTCGTGATGGGGCTTCTGGTTTGTTTATGGCCGCGAGTAATTTCCCTAAATCACGTGAAACACGTGCACCATTA
GTGGAAGAGCTGGTTCGTTTTAAAGACCGTATTTCACCTAAATTCCAGTATCTGGCATCAGCACCGGTTGCTGATCCTGA
TGGCAAGCCGACAGTAGTTCGTTTTAGTCGTAAGACAAAAGAAAACTACGTTCGTACCGAAGTGGACGGTAAACCTTCAG
GTTGGACCGCTTTGTACATCGACGGTAAGTGGGAAGTTACGGATAAACGTAAAAAGCCTAAAGAGAAGTAA

Upstream 100 bases:

>100_bases
TAGTAAAAAAAGAAGCAATGAATTTAGAATATCATGCGTAAAAGCCCACAAGTAAAATTGTTGGTGTTGAAGCATCGTGA
TTAAGGACGTTAGAGTCAAT

Downstream 100 bases:

>100_bases
CTGAATGTGTGACCCGATCTGATATGGGTTGACATTTTATTGATGAAAAAAGCAGCCATTAGGCTGCTTTTTTTTGATCT
GAATATGACTAATTATGCTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MGKSLVIVESPAKAKTINKYLGKDFVVKSSVGHVRDLPTAGQSTGQKAAAVSTKGMSPEDKARIKKEKDRKALIKKMGIN
PYQEWEANYQILPGKEKVVAELQKLAENADHVYLATDLDREGEAIAWHLREIIGGDETRYKRVVFNEITKNAIQQAFETP
GELNIDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREINAFIPEEFWDVHADTKTAEKTDF
RLQVAQKDGVAFKPDNEAQTQAAVSVLEKAQYEVCKREDRPTKSKPSAPYITSTLQQAASTRLGYGVKKTMMLAQRLYEA
GYITYMRTDSTNLSSEAVEAAREYIGSEFGAQYLPPKALVYGSKEGAQEAHEAIRPSSVDVKSEDLNGVDADAHKLYSLI
WNQFVACQMTPAQYDSTTVSVKADEYTLKAKGRILKFDGWTRVQRPMGKNEDTILPAVQVGEKLDLIALDPKQHFTKPPA
RFTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVEQRRFYAEKMGEIVTDRLDGSFNDLMNYDFTARMEQKLDQIAE
GEASWKGVLDNFFEDFTGDLEKADLDEDAGGMKPNHIVETDIECPTCSRKMGIRTASTGVFLGCSGYALPPKERCKTTIN
LGDEDGIINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKMHVCGNNPNCEGYVVEHGEFKVKGYDGPLVECDKCSS
DMVLKNGRFGKYMDCTSEDCKNTRKILKNGEVAPPKEDPVHFPELPCENSDAYFVLRDGASGLFMAASNFPKSRETRAPL
VEELVRFKDRISPKFQYLASAPVADPDGKPTVVRFSRKTKENYVRTEVDGKPSGWTALYIDGKWEVTDKRKKPKEK

Sequences:

>Translated_876_residues
MGKSLVIVESPAKAKTINKYLGKDFVVKSSVGHVRDLPTAGQSTGQKAAAVSTKGMSPEDKARIKKEKDRKALIKKMGIN
PYQEWEANYQILPGKEKVVAELQKLAENADHVYLATDLDREGEAIAWHLREIIGGDETRYKRVVFNEITKNAIQQAFETP
GELNIDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREINAFIPEEFWDVHADTKTAEKTDF
RLQVAQKDGVAFKPDNEAQTQAAVSVLEKAQYEVCKREDRPTKSKPSAPYITSTLQQAASTRLGYGVKKTMMLAQRLYEA
GYITYMRTDSTNLSSEAVEAAREYIGSEFGAQYLPPKALVYGSKEGAQEAHEAIRPSSVDVKSEDLNGVDADAHKLYSLI
WNQFVACQMTPAQYDSTTVSVKADEYTLKAKGRILKFDGWTRVQRPMGKNEDTILPAVQVGEKLDLIALDPKQHFTKPPA
RFTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVEQRRFYAEKMGEIVTDRLDGSFNDLMNYDFTARMEQKLDQIAE
GEASWKGVLDNFFEDFTGDLEKADLDEDAGGMKPNHIVETDIECPTCSRKMGIRTASTGVFLGCSGYALPPKERCKTTIN
LGDEDGIINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKMHVCGNNPNCEGYVVEHGEFKVKGYDGPLVECDKCSS
DMVLKNGRFGKYMDCTSEDCKNTRKILKNGEVAPPKEDPVHFPELPCENSDAYFVLRDGASGLFMAASNFPKSRETRAPL
VEELVRFKDRISPKFQYLASAPVADPDGKPTVVRFSRKTKENYVRTEVDGKPSGWTALYIDGKWEVTDKRKKPKEK
>Mature_875_residues
GKSLVIVESPAKAKTINKYLGKDFVVKSSVGHVRDLPTAGQSTGQKAAAVSTKGMSPEDKARIKKEKDRKALIKKMGINP
YQEWEANYQILPGKEKVVAELQKLAENADHVYLATDLDREGEAIAWHLREIIGGDETRYKRVVFNEITKNAIQQAFETPG
ELNIDGVNAQQARRFMDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREINAFIPEEFWDVHADTKTAEKTDFR
LQVAQKDGVAFKPDNEAQTQAAVSVLEKAQYEVCKREDRPTKSKPSAPYITSTLQQAASTRLGYGVKKTMMLAQRLYEAG
YITYMRTDSTNLSSEAVEAAREYIGSEFGAQYLPPKALVYGSKEGAQEAHEAIRPSSVDVKSEDLNGVDADAHKLYSLIW
NQFVACQMTPAQYDSTTVSVKADEYTLKAKGRILKFDGWTRVQRPMGKNEDTILPAVQVGEKLDLIALDPKQHFTKPPAR
FTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVEQRRFYAEKMGEIVTDRLDGSFNDLMNYDFTARMEQKLDQIAEG
EASWKGVLDNFFEDFTGDLEKADLDEDAGGMKPNHIVETDIECPTCSRKMGIRTASTGVFLGCSGYALPPKERCKTTINL
GDEDGIINVLEEDVETAALRAKKRCPICETAMDAYLIDDKRKMHVCGNNPNCEGYVVEHGEFKVKGYDGPLVECDKCSSD
MVLKNGRFGKYMDCTSEDCKNTRKILKNGEVAPPKEDPVHFPELPCENSDAYFVLRDGASGLFMAASNFPKSRETRAPLV
EELVRFKDRISPKFQYLASAPVADPDGKPTVVRFSRKTKENYVRTEVDGKPSGWTALYIDGKWEVTDKRKKPKEK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=618, Percent_Identity=25.2427184466019, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI4507635, Length=604, Percent_Identity=25.6622516556291, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1787529, Length=866, Percent_Identity=71.3625866050808, Blast_Score=1306, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=555, Percent_Identity=23.6036036036036, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=597, Percent_Identity=25.2931323283082, Blast_Score=134, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=509, Percent_Identity=25.3438113948919, Blast_Score=119, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=579, Percent_Identity=24.006908462867, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24585251, Length=631, Percent_Identity=24.0887480190174, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640096, Length=503, Percent_Identity=25.6461232604374, Blast_Score=124, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97969; Mature: 97838

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGKDFVVKSSVGHVRDLPTAGQSTGQKAAAVSTKGMSPED
CCCCEEEEECCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCCHHEEECCCCCCHH
KARIKKEKDRKALIKKMGINPYQEWEANYQILPGKEKVVAELQKLAENADHVYLATDLDR
HHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCC
EGEAIAWHLREIIGGDETRYKRVVFNEITKNAIQQAFETPGELNIDGVNAQQARRFMDRV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
VGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREINAFIPEEFWDVHADTKTAEKTDF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCE
RLQVAQKDGVAFKPDNEAQTQAAVSVLEKAQYEVCKREDRPTKSKPSAPYITSTLQQAAS
EEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TRLGYGVKKTMMLAQRLYEAGYITYMRTDSTNLSSEAVEAAREYIGSEFGAQYLPPKALV
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHEE
YGSKEGAQEAHEAIRPSSVDVKSEDLNGVDADAHKLYSLIWNQFVACQMTPAQYDSTTVS
ECCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEE
VKADEYTLKAKGRILKFDGWTRVQRPMGKNEDTILPAVQVGEKLDLIALDPKQHFTKPPA
EEECCEEEEECCCEEEECCCHHHHCCCCCCCCCCCHHHHCCCCCCEEEECCHHHCCCCCH
RFTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVEQRRFYAEKMGEIVTDRLDGSFN
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCHH
DLMNYDFTARMEQKLDQIAEGEASWKGVLDNFFEDFTGDLEKADLDEDAGGMKPNHIVET
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEEEC
DIECPTCSRKMGIRTASTGVFLGCSGYALPPKERCKTTINLGDEDGIINVLEEDVETAAL
CCCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHCCEEECCCCCCCHHHHHHHHHHHHHH
RAKKRCPICETAMDAYLIDDKRKMHVCGNNPNCEGYVVEHGEFKVKGYDGPLVECDKCSS
HHHCCCCHHHHHHHHEEECCCCEEEEECCCCCCCEEEEECCEEEEECCCCCCCCCCCCCC
DMVLKNGRFGKYMDCTSEDCKNTRKILKNGEVAPPKEDPVHFPELPCENSDAYFVLRDGA
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFMAASNFPKSRETRAPLVEELVRFKDRISPKFQYLASAPVADPDGKPTVVRFSRKTK
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEECHHHH
ENYVRTEVDGKPSGWTALYIDGKWEVTDKRKKPKEK
HCCEEEECCCCCCCEEEEEECCEEEECCCCCCCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGKDFVVKSSVGHVRDLPTAGQSTGQKAAAVSTKGMSPED
CCCEEEEECCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCCHHEEECCCCCCHH
KARIKKEKDRKALIKKMGINPYQEWEANYQILPGKEKVVAELQKLAENADHVYLATDLDR
HHHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCC
EGEAIAWHLREIIGGDETRYKRVVFNEITKNAIQQAFETPGELNIDGVNAQQARRFMDRV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
VGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREINAFIPEEFWDVHADTKTAEKTDF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCE
RLQVAQKDGVAFKPDNEAQTQAAVSVLEKAQYEVCKREDRPTKSKPSAPYITSTLQQAAS
EEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TRLGYGVKKTMMLAQRLYEAGYITYMRTDSTNLSSEAVEAAREYIGSEFGAQYLPPKALV
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHEE
YGSKEGAQEAHEAIRPSSVDVKSEDLNGVDADAHKLYSLIWNQFVACQMTPAQYDSTTVS
ECCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEE
VKADEYTLKAKGRILKFDGWTRVQRPMGKNEDTILPAVQVGEKLDLIALDPKQHFTKPPA
EEECCEEEEECCCEEEECCCHHHHCCCCCCCCCCCHHHHCCCCCCEEEECCHHHCCCCCH
RFTEAALVKELEKRGIGRPSTYASIISTIQDRGYVKVEQRRFYAEKMGEIVTDRLDGSFN
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCHH
DLMNYDFTARMEQKLDQIAEGEASWKGVLDNFFEDFTGDLEKADLDEDAGGMKPNHIVET
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEEEC
DIECPTCSRKMGIRTASTGVFLGCSGYALPPKERCKTTINLGDEDGIINVLEEDVETAAL
CCCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHCCEEECCCCCCCHHHHHHHHHHHHHH
RAKKRCPICETAMDAYLIDDKRKMHVCGNNPNCEGYVVEHGEFKVKGYDGPLVECDKCSS
HHHCCCCHHHHHHHHEEECCCCEEEEECCCCCCCEEEEECCEEEEECCCCCCCCCCCCCC
DMVLKNGRFGKYMDCTSEDCKNTRKILKNGEVAPPKEDPVHFPELPCENSDAYFVLRDGA
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFMAASNFPKSRETRAPLVEELVRFKDRISPKFQYLASAPVADPDGKPTVVRFSRKTK
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEECHHHH
ENYVRTEVDGKPSGWTALYIDGKWEVTDKRKKPKEK
HCCEEEECCCCCCCEEEEEECCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]