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The map label for this gene is gloA [H]

Identifier: 218708966

GI number: 218708966

Start: 1004074

End: 1004490

Strand: Reverse

Name: gloA [H]

Synonym: VS_0970

Alternate gene names: 218708966

Gene position: 1004490-1004074 (Counterclockwise)

Preceding gene: 218708967

Following gene: 218708965

Centisome position: 30.45

GC content: 46.52

Gene sequence:

>417_bases
ATGTCAAACGGCCGTATTTTACACACCATGCTACGCGTTGGTGATCTAGACAAATCTATCGAGTTCTACACCAATGTAAT
GGGCATGCAGCTGTTACGTAAGAACGAGAATAAAGAGTACGAATACACACTGGCTTTCGTTGGCTTTGGTGACGAATCTC
AAGGCGCTGTGATTGAGCTGACTTACAACTGGGGCACGACTGAATATGACCTTGGCTCAGCTTTTGGTCACGTTGCTATC
GGTGTTGATGACATCTACACAACATGCGACGCGATTAAAGCAGCAGGCGGTAATGTGACACGTGAAGCGGGCCCTGTAAA
AGGCGGCTCTACTCACATCGCATTCGTTAAAGATCCTGACGGCTACATGATTGAGCTTATTCAGAACAAACAAGCAAGTG
CTGGTCTAGAAGGTTAA

Upstream 100 bases:

>100_bases
AAGAAGCTAAGAAGCTAAGAAGCTAAACAATGCCGCATTTTTCTAGCGGCATAAAACAGAATTACAAAAACGTATACATA
ATTAAAGCTAGGAGCAAATC

Downstream 100 bases:

>100_bases
CCCTTCGTAGCCTCTGCAATATTTGGCTATGGAATAAACACTATAAGCGAGCATAAAGCTCGCTTTTTTTGTAGCTCTAT
TAATAATCGATATTCTTTAC

Product: putative lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MSNGRILHTMLRVGDLDKSIEFYTNVMGMQLLRKNENKEYEYTLAFVGFGDESQGAVIELTYNWGTTEYDLGSAFGHVAI
GVDDIYTTCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPDGYMIELIQNKQASAGLEG

Sequences:

>Translated_138_residues
MSNGRILHTMLRVGDLDKSIEFYTNVMGMQLLRKNENKEYEYTLAFVGFGDESQGAVIELTYNWGTTEYDLGSAFGHVAI
GVDDIYTTCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPDGYMIELIQNKQASAGLEG
>Mature_137_residues
SNGRILHTMLRVGDLDKSIEFYTNVMGMQLLRKNENKEYEYTLAFVGFGDESQGAVIELTYNWGTTEYDLGSAFGHVAIG
VDDIYTTCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPDGYMIELIQNKQASAGLEG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=153, Percent_Identity=33.9869281045752, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=65.9090909090909, Blast_Score=191, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17552228, Length=144, Percent_Identity=28.4722222222222, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=138, Percent_Identity=37.6811594202899, Blast_Score=94, Evalue=6e-21,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=36.1111111111111, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920404, Length=143, Percent_Identity=32.1678321678322, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14992; Mature: 14861

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGRILHTMLRVGDLDKSIEFYTNVMGMQLLRKNENKEYEYTLAFVGFGDESQGAVIEL
CCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEE
TYNWGTTEYDLGSAFGHVAIGVDDIYTTCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPD
EECCCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
GYMIELIQNKQASAGLEG
CEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SNGRILHTMLRVGDLDKSIEFYTNVMGMQLLRKNENKEYEYTLAFVGFGDESQGAVIEL
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEE
TYNWGTTEYDLGSAFGHVAIGVDDIYTTCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPD
EECCCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
GYMIELIQNKQASAGLEG
CEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]