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The map label for this gene is nth [H]

Identifier: 218708967

GI number: 218708967

Start: 1004630

End: 1005265

Strand: Reverse

Name: nth [H]

Synonym: VS_0971

Alternate gene names: 218708967

Gene position: 1005265-1004630 (Counterclockwise)

Preceding gene: 218708968

Following gene: 218708966

Centisome position: 30.47

GC content: 45.75

Gene sequence:

>636_bases
ATGAACAATGTAAAACGAGTAGAAATACTTGAGCGTTTAAGAGAAAACAATCCAAAGCCAGAAACCGAGCTTAATTGGAA
CAGCCCTTTCGAGTTGCTGATCGCAGTGCTCTTATCTGCGCAGGCAACCGATGTCAGCGTTAACAAAGCCACTGATAAGC
TTTATCCTGTGGCTAATACTCCACAGGCTATTTTCGACCTAGGTGTCGATGGCCTAAAAGAGTACATCAAAACCATCGGC
CTATTTAATTCGAAAGCAGAAAACACCATCAAAACGTGTCGCATGCTGCTTGACCTACATAATGGTGAAGTACCGGAAGA
TCGCGCTGCATTAGAGGCCCTTCCAGGCGTTGGCCGCAAAACTGCAAACGTAGTGTTAAATACCGCCTTCGGTTGGCCAA
CGATTGCCGTTGATACTCACATCTATCGTGTATCAAACCGCACTAAGCTAGCGATGGGTAAAACCGTCGACGATGTCGAA
GCAAAGCTACTTAAAGTCATCCCAAAAGAATTCAAACTGGATGTCCACCATTGGCTCATTCTTCATGGACGTTACACCTG
TGTCGCACGTAAACCACGCTGTGGCAGCTGTATCATTGAAGACTTGTGTGAGTTCAAGGAAAAAACCGACGTCTAG

Upstream 100 bases:

>100_bases
AACAGTACATATAATAATGACGCTCATAAGAGCGCGTAACCGATGATAAAGCAGAAGCAATCCAAGCTTCATGCCCTAAC
TATTTGAAAGTAATGTCGCT

Downstream 100 bases:

>100_bases
GCTAGCTCTAAGCGAATGAAGCTAAGAAGCTAAGAAGCTAAGAAGCTAAGAAGCTAAGAAGCTAAACAATGCCGCATTTT
TCTAGCGGCATAAAACAGAA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNNVKRVEILERLRENNPKPETELNWNSPFELLIAVLLSAQATDVSVNKATDKLYPVANTPQAIFDLGVDGLKEYIKTIG
LFNSKAENTIKTCRMLLDLHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIYRVSNRTKLAMGKTVDDVE
AKLLKVIPKEFKLDVHHWLILHGRYTCVARKPRCGSCIIEDLCEFKEKTDV

Sequences:

>Translated_211_residues
MNNVKRVEILERLRENNPKPETELNWNSPFELLIAVLLSAQATDVSVNKATDKLYPVANTPQAIFDLGVDGLKEYIKTIG
LFNSKAENTIKTCRMLLDLHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIYRVSNRTKLAMGKTVDDVE
AKLLKVIPKEFKLDVHHWLILHGRYTCVARKPRCGSCIIEDLCEFKEKTDV
>Mature_211_residues
MNNVKRVEILERLRENNPKPETELNWNSPFELLIAVLLSAQATDVSVNKATDKLYPVANTPQAIFDLGVDGLKEYIKTIG
LFNSKAENTIKTCRMLLDLHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIYRVSNRTKLAMGKTVDDVE
AKLLKVIPKEFKLDVHHWLILHGRYTCVARKPRCGSCIIEDLCEFKEKTDV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=32.0224719101124, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=81.9905213270142, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=30.4812834224599, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23709; Mature: 23709

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVKRVEILERLRENNPKPETELNWNSPFELLIAVLLSAQATDVSVNKATDKLYPVANT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEECCCCC
PQAIFDLGVDGLKEYIKTIGLFNSKAENTIKTCRMLLDLHNGEVPEDRAALEALPGVGRK
CHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIYRVSNRTKLAMGKTVDDVEAKLLKVIPKEFKLDVHHWLI
HHHEEEECCCCCCEEEECCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEE
LHGRYTCVARKPRCGSCIIEDLCEFKEKTDV
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNVKRVEILERLRENNPKPETELNWNSPFELLIAVLLSAQATDVSVNKATDKLYPVANT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEECCCCC
PQAIFDLGVDGLKEYIKTIGLFNSKAENTIKTCRMLLDLHNGEVPEDRAALEALPGVGRK
CHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIYRVSNRTKLAMGKTVDDVEAKLLKVIPKEFKLDVHHWLI
HHHEEEECCCCCCEEEECCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEE
LHGRYTCVARKPRCGSCIIEDLCEFKEKTDV
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]