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The map label for this gene is rnt [H]

Identifier: 218708963

GI number: 218708963

Start: 1001637

End: 1002362

Strand: Reverse

Name: rnt [H]

Synonym: VS_0967

Alternate gene names: 218708963

Gene position: 1002362-1001637 (Counterclockwise)

Preceding gene: 218708965

Following gene: 218708962

Centisome position: 30.38

GC content: 43.11

Gene sequence:

>726_bases
ATGATTTATTGCCCTTCCCTAGTTTTTATCACTGCTATTATCTGCAATAATGCTGCCTTAATCACACATATTTGGGCTAA
CATGACTGTAGAAAACGAAGCTCTGACCCTTAAAAAACGCTTTCGTGGCTATTTCCCAGTGGTCATCGACGTGGAAACCG
CAGGGTTTAACGCTGAAACCGATGCATTATTAGAAATCTGTGCCATTACACTAAGAATGGATGAGAACGGAGATCTGCAC
CCTGCATCAACGCTTCATTTTCACATTGAGCCTTTTGAAGGCGCAAATATAGAGCAGGCAGCATTAGACTTTAACGGAAT
TAAAGACCCATTTAGCCCATTACGTGGTGCTGTGTCGGAACAAGAAGCCCTTAAAGAAATCTACAAGCTTGTGAGAAAAG
AACAAAAAGCTTCAGATTGCAGTCGCGCAATCATGGTCGCTCACAATGCTACGTTCGATTTAAACTTCGTTAATGCAGCA
AGTGAGCGTTGTAAGCTTAAACGCGTCCCTTTCCATCCATTTGCAACTTTTGATACTGCAGCCCTTAGTGGTCTTGCCTA
CGGTCAAACCGTTTTGGCTAAAGCTTGCCGCACTGCGGGGATGGAATTTGACAACAAAGAAGCACACTCTGCTTTGTATG
ATACGCAAAAAACCACAGAGTTATTTTGCGGCATAGTAAACAAATGGAAAGCCCTTGGTGGTTGGCCTCTTGTTGACGAA
GAATAA

Upstream 100 bases:

>100_bases
AGTATCTCTTAAGCAATTCGTTTAACAATGCAGCCAATTGCCGCATTTTTCCATCACGTTTGAATATGCAATTATCAAGC
CGAAATAGGTCGGCTAATTT

Downstream 100 bases:

>100_bases
CAAATAGAGTCGGTAAACGTTCACTCGTATTGCCTTCATAAAAAACACAACATCCCGAGAATAATATGAATCCTGTTGTA
ATTTCAGTTTGCATCATGCT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIYCPSLVFITAIICNNAALITHIWANMTVENEALTLKKRFRGYFPVVIDVETAGFNAETDALLEICAITLRMDENGDLH
PASTLHFHIEPFEGANIEQAALDFNGIKDPFSPLRGAVSEQEALKEIYKLVRKEQKASDCSRAIMVAHNATFDLNFVNAA
SERCKLKRVPFHPFATFDTAALSGLAYGQTVLAKACRTAGMEFDNKEAHSALYDTQKTTELFCGIVNKWKALGGWPLVDE
E

Sequences:

>Translated_241_residues
MIYCPSLVFITAIICNNAALITHIWANMTVENEALTLKKRFRGYFPVVIDVETAGFNAETDALLEICAITLRMDENGDLH
PASTLHFHIEPFEGANIEQAALDFNGIKDPFSPLRGAVSEQEALKEIYKLVRKEQKASDCSRAIMVAHNATFDLNFVNAA
SERCKLKRVPFHPFATFDTAALSGLAYGQTVLAKACRTAGMEFDNKEAHSALYDTQKTTELFCGIVNKWKALGGWPLVDE
E
>Mature_241_residues
MIYCPSLVFITAIICNNAALITHIWANMTVENEALTLKKRFRGYFPVVIDVETAGFNAETDALLEICAITLRMDENGDLH
PASTLHFHIEPFEGANIEQAALDFNGIKDPFSPLRGAVSEQEALKEIYKLVRKEQKASDCSRAIMVAHNATFDLNFVNAA
SERCKLKRVPFHPFATFDTAALSGLAYGQTVLAKACRTAGMEFDNKEAHSALYDTQKTTELFCGIVNKWKALGGWPLVDE
E

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=208, Percent_Identity=71.6346153846154, Blast_Score=300, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 26736; Mature: 26736

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYCPSLVFITAIICNNAALITHIWANMTVENEALTLKKRFRGYFPVVIDVETAGFNAET
CEECCHHHHHHHHHHCCCCEEEEHHHCCEECCHHHHHHHHHCCCCCEEEEEECCCCCCCH
DALLEICAITLRMDENGDLHPASTLHFHIEPFEGANIEQAALDFNGIKDPFSPLRGAVSE
HHHHHHHHHEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHCCCCH
QEALKEIYKLVRKEQKASDCSRAIMVAHNATFDLNFVNAASERCKLKRVPFHPFATFDTA
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEHHHHHHHHEEECCCCCCCCCHHHH
ALSGLAYGQTVLAKACRTAGMEFDNKEAHSALYDTQKTTELFCGIVNKWKALGGWPLVDE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
E
C
>Mature Secondary Structure
MIYCPSLVFITAIICNNAALITHIWANMTVENEALTLKKRFRGYFPVVIDVETAGFNAET
CEECCHHHHHHHHHHCCCCEEEEHHHCCEECCHHHHHHHHHCCCCCEEEEEECCCCCCCH
DALLEICAITLRMDENGDLHPASTLHFHIEPFEGANIEQAALDFNGIKDPFSPLRGAVSE
HHHHHHHHHEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHCCCCH
QEALKEIYKLVRKEQKASDCSRAIMVAHNATFDLNFVNAASERCKLKRVPFHPFATFDTA
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEHHHHHHHHEEECCCCCCCCCHHHH
ALSGLAYGQTVLAKACRTAGMEFDNKEAHSALYDTQKTTELFCGIVNKWKALGGWPLVDE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA