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The map label for this gene is phoB [H]

Identifier: 218708922

GI number: 218708922

Start: 948682

End: 950259

Strand: Reverse

Name: phoB [H]

Synonym: VS_0924

Alternate gene names: 218708922

Gene position: 950259-948682 (Counterclockwise)

Preceding gene: 218708923

Following gene: 218708917

Centisome position: 28.8

GC content: 45.31

Gene sequence:

>1578_bases
ATGAAGCACATTATAAAACCAATCATTGCCGCAGTGGCAACCTCAACACTTTCATTCAACGTACTTTCAGCAGAAATCAA
AAACGTCATTCTGATGATTGGCGATGGAATGGGACCTCAACAAGTTGGCCTGTTAGAAACCTACGCAAACCAAGCACCAA
ACTCGATCTATAAAGGGAACAAAACCGCCCTTTATCAACTTGCTCAAGAAGGGGTTATTGGTTCATCCCTGACTCACCCA
GAAGATGCAATTGTGGTCGATTCTGCTTGTTCAGCCACCATGCTTGCAACCGGTATCTACAGTGGTTCAGAAGTGATTGG
CATTGATTCTCAGGGCAATCATGTTGAGACGGTTCTTGAGAAAGCTAAAAAGGCAGGTAAAGCAACAGGACTCGTGTCCG
ATACGCGCTTAACTCACGCCACGCCTGCTGCATTTGCTGCTCACCAACCTCACCGTTCGCTAGAAAACCAAATTGCTAAC
GACATGCTAGAAACTGGTGTTGATGTAATGCTTTCGGGAGGGCTACGTCATTGGATCCCTAAATCGACCAACGACAAAGG
TGAAACCTATAAGCAACTTGAAAAACTGACTCAAGGTGATGTTTACCTAAAATCAAAACGTAAAGACGACCGTAACCTTC
TTGCAGAGGCAGAGAAAGACGGCTACCAACTGGCATTTAACCGCAGCATGCTAGAAGATGCTAAAAGCGATAAGCTACTT
GGCCTGTTCGCCTACTCAGGCATGGATGACGGCATCGCTTACAGCAACAAGAAAGAGAGTGGCGAACGTACTCAACCAAG
TTTGAAAGAGATGACACAAAAAGCCCTCAACATCTTATCCAAAGATGAAGACGGCTTTTTCCTAATGGTCGAAGGTGGCC
AAATAGATTGGGCGGGACACAGTAACGATGCCGGCACTATGCTGCATGAACTGCTCAAGTTTGATGAAGCGATCCAAACT
GTGTATGAATGGGCAAAAAATCGTGAAGACACGATCGTGATTGTGACCGCAGACCACGAAACAGGATCTTTTGGATTCAG
CTACTCTTCTAACGACCTACCAATACCACAAAAACGTTCTGGTGAAGCCTTCGCCGACCGCGACTATGCACCTAACTTCA
ACTTTGGCGCATTCGATATTCTTGATGGTTTATATAATCAAAAGCAAAGCTACTACGGCATGATCAGCGAATTTCAGAAG
CTGGATAAAGCGCAGCAAACACCTGAAAAACTGGCTGAGATCGTCAACAAGAATAGTGAGTTCCCTATTACAGCGGAACA
AGCGAAAAACGTATTAGCGAGTAAGCCGAACCCATACCGATTGGCTCAACACAAATACTTATCGGCAGAAGAAGTGCCTG
CTATCAACGATTTCGATGCATTCTTCCCTTATAACGACCGCGGAAACTTGCTTGCTCGTGAACAGGCAACAAGTCAAAAC
ATCGTTTGGGGTACAGGTACACATACTCACACACCAGTGAACGTGTTTGCTTGGGGCCCAGCAGAGAAAATACTGCCCGT
TTCAAAAATCATGCACCACTCAGAACTGGGTGAGTACATTAAACAACAAGTAAACTAG

Upstream 100 bases:

>100_bases
TGGAGTTATTGCTCAATTTCACATTGATGAAATAAATATGAAACGACTTTGACACAAATCGATGTTCTAATCATCCCGCT
TATATAAAAAGGGATAACCA

Downstream 100 bases:

>100_bases
CCTGAATCTTTTTTACTCGTTTTACTTAGCGCCCTACGGGGCGTTTTTTGTTTTAAGCCTTTAAGTTAAGTAATTAAGTA
AATTTAGGCGACGCCAAATA

Product: alkaline phosphatase III precursor

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKHIIKPIIAAVATSTLSFNVLSAEIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGNKTALYQLAQEGVIGSSLTHP
EDAIVVDSACSATMLATGIYSGSEVIGIDSQGNHVETVLEKAKKAGKATGLVSDTRLTHATPAAFAAHQPHRSLENQIAN
DMLETGVDVMLSGGLRHWIPKSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLAEAEKDGYQLAFNRSMLEDAKSDKLL
GLFAYSGMDDGIAYSNKKESGERTQPSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGHSNDAGTMLHELLKFDEAIQT
VYEWAKNREDTIVIVTADHETGSFGFSYSSNDLPIPQKRSGEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQK
LDKAQQTPEKLAEIVNKNSEFPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAREQATSQN
IVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQVN

Sequences:

>Translated_525_residues
MKHIIKPIIAAVATSTLSFNVLSAEIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGNKTALYQLAQEGVIGSSLTHP
EDAIVVDSACSATMLATGIYSGSEVIGIDSQGNHVETVLEKAKKAGKATGLVSDTRLTHATPAAFAAHQPHRSLENQIAN
DMLETGVDVMLSGGLRHWIPKSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLAEAEKDGYQLAFNRSMLEDAKSDKLL
GLFAYSGMDDGIAYSNKKESGERTQPSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGHSNDAGTMLHELLKFDEAIQT
VYEWAKNREDTIVIVTADHETGSFGFSYSSNDLPIPQKRSGEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQK
LDKAQQTPEKLAEIVNKNSEFPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAREQATSQN
IVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQVN
>Mature_525_residues
MKHIIKPIIAAVATSTLSFNVLSAEIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGNKTALYQLAQEGVIGSSLTHP
EDAIVVDSACSATMLATGIYSGSEVIGIDSQGNHVETVLEKAKKAGKATGLVSDTRLTHATPAAFAAHQPHRSLENQIAN
DMLETGVDVMLSGGLRHWIPKSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLAEAEKDGYQLAFNRSMLEDAKSDKLL
GLFAYSGMDDGIAYSNKKESGERTQPSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGHSNDAGTMLHELLKFDEAIQT
VYEWAKNREDTIVIVTADHETGSFGFSYSSNDLPIPQKRSGEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQK
LDKAQQTPEKLAEIVNKNSEFPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAREQATSQN
IVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQVN

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=354, Percent_Identity=32.4858757062147, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI94721246, Length=354, Percent_Identity=31.638418079096, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI116734717, Length=347, Percent_Identity=32.2766570605187, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI157266292, Length=354, Percent_Identity=31.638418079096, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI294660770, Length=343, Percent_Identity=31.4868804664723, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI294660772, Length=297, Percent_Identity=32.6599326599327, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI48994877, Length=365, Percent_Identity=32.0547945205479, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=347, Percent_Identity=37.463976945245, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI21355981, Length=367, Percent_Identity=31.3351498637602, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24651554, Length=348, Percent_Identity=34.4827586206897, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21358067, Length=354, Percent_Identity=31.3559322033898, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355151, Length=358, Percent_Identity=32.122905027933, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI18859923, Length=346, Percent_Identity=31.5028901734104, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921912, Length=354, Percent_Identity=31.0734463276836, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI85725200, Length=367, Percent_Identity=29.700272479564, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI85815833, Length=367, Percent_Identity=29.700272479564, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585213, Length=355, Percent_Identity=31.2676056338028, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24651556, Length=301, Percent_Identity=35.8803986710963, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24657835, Length=355, Percent_Identity=29.2957746478873, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24657827, Length=356, Percent_Identity=31.1797752808989, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21355149, Length=351, Percent_Identity=27.9202279202279, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24657842, Length=356, Percent_Identity=28.9325842696629, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 57867; Mature: 57867

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIIKPIIAAVATSTLSFNVLSAEIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHCCC
KTALYQLAQEGVIGSSLTHPEDAIVVDSACSATMLATGIYSGSEVIGIDSQGNHVETVLE
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHH
KAKKAGKATGLVSDTRLTHATPAAFAAHQPHRSLENQIANDMLETGVDVMLSGGLRHWIP
HHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHEECCCHHHCCC
KSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLAEAEKDGYQLAFNRSMLEDAKSDKLL
CCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHHCCCCCEE
GLFAYSGMDDGIAYSNKKESGERTQPSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGH
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCC
SNDAGTMLHELLKFDEAIQTVYEWAKNREDTIVIVTADHETGSFGFSYSSNDLPIPQKRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCC
GEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQKLDKAQQTPEKLAEIVNKNSE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
FPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAREQATSQN
CCEEHHHHHHHHHCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCCCCCCCEEEHHHCCCCC
IVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQVN
EEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHIIKPIIAAVATSTLSFNVLSAEIKNVILMIGDGMGPQQVGLLETYANQAPNSIYKGN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHCCC
KTALYQLAQEGVIGSSLTHPEDAIVVDSACSATMLATGIYSGSEVIGIDSQGNHVETVLE
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHH
KAKKAGKATGLVSDTRLTHATPAAFAAHQPHRSLENQIANDMLETGVDVMLSGGLRHWIP
HHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHEECCCHHHCCC
KSTNDKGETYKQLEKLTQGDVYLKSKRKDDRNLLAEAEKDGYQLAFNRSMLEDAKSDKLL
CCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHHCCCCCEE
GLFAYSGMDDGIAYSNKKESGERTQPSLKEMTQKALNILSKDEDGFFLMVEGGQIDWAGH
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCC
SNDAGTMLHELLKFDEAIQTVYEWAKNREDTIVIVTADHETGSFGFSYSSNDLPIPQKRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCC
GEAFADRDYAPNFNFGAFDILDGLYNQKQSYYGMISEFQKLDKAQQTPEKLAEIVNKNSE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
FPITAEQAKNVLASKPNPYRLAQHKYLSAEEVPAINDFDAFFPYNDRGNLLAREQATSQN
CCEEHHHHHHHHHCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCCCCCCCEEEHHHCCCCC
IVWGTGTHTHTPVNVFAWGPAEKILPVSKIMHHSELGEYIKQQVN
EEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]