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The map label for this gene is iscA [H]

Identifier: 218708631

GI number: 218708631

Start: 632287

End: 632610

Strand: Direct

Name: iscA [H]

Synonym: VS_0609

Alternate gene names: 218708631

Gene position: 632287-632610 (Clockwise)

Preceding gene: 218708630

Following gene: 218708632

Centisome position: 19.16

GC content: 44.14

Gene sequence:

>324_bases
ATGGCCATCACCATGACAGATACGGCAGCAAGCCGAGTTCAAGCTTTCCTAGATAACCGTGGTAAAGGTCTCGGGTTACG
CTTAGCAGTAAAAACTACTGGCTGTTCGGGTATGGCGTATGTACTGGAATTCGTTGACGAGCTTAACGAAGAAGACGAAG
TGTTTGAGCATTCAGGTGTTAAGGTCATCATTGATCCAAAGAGCCTAGTATACCTTGATGGTACTGAGCTTGATTATGTA
AAAGAAGGCCTAAACGAAGGTTTTGAATTCAACAACCCGAACGCAAAAGGCGAATGTGGTTGTGGTGAGAGCTTCAATGT
TTAA

Upstream 100 bases:

>100_bases
CGCTTCAATACCGTAATTACGTCGACAAAAAAGTAATATTTGGGAGCATCCTTTGTGCTCCCCCTGAGTTCTCAATTTAT
ATAAAACACAAGGTTGTAGT

Downstream 100 bases:

>100_bases
ACGCTTGAATCGTTAAGCGTTGTAAGCGAAGAATAAAATTAGGCTCTGAACAAAGAGCCTAAACTTAGGACCACCTTTAC
ATGAATCATTTCGAATTATT

Product: HesB family protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAITMTDTAASRVQAFLDNRGKGLGLRLAVKTTGCSGMAYVLEFVDELNEEDEVFEHSGVKVIIDPKSLVYLDGTELDYV
KEGLNEGFEFNNPNAKGECGCGESFNV

Sequences:

>Translated_107_residues
MAITMTDTAASRVQAFLDNRGKGLGLRLAVKTTGCSGMAYVLEFVDELNEEDEVFEHSGVKVIIDPKSLVYLDGTELDYV
KEGLNEGFEFNNPNAKGECGCGESFNV
>Mature_106_residues
AITMTDTAASRVQAFLDNRGKGLGLRLAVKTTGCSGMAYVLEFVDELNEEDEVFEHSGVKVIIDPKSLVYLDGTELDYVK
EGLNEGFEFNNPNAKGECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=42.4528301886792, Blast_Score=103, Evalue=2e-23,
Organism=Homo sapiens, GI160420328, Length=106, Percent_Identity=35.8490566037736, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=71.9626168224299, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=45.7142857142857, Blast_Score=111, Evalue=1e-26,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=43.6363636363636, Blast_Score=85, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=42.4528301886792, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=42.4528301886792, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=36.7924528301887, Blast_Score=68, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=41.9047619047619, Blast_Score=95, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=42.4528301886792, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24649697, Length=83, Percent_Identity=40.9638554216867, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11631; Mature: 11500

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITMTDTAASRVQAFLDNRGKGLGLRLAVKTTGCSGMAYVLEFVDELNEEDEVFEHSGV
CEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHCCCC
KVIIDPKSLVYLDGTELDYVKEGLNEGFEFNNPNAKGECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AITMTDTAASRVQAFLDNRGKGLGLRLAVKTTGCSGMAYVLEFVDELNEEDEVFEHSGV
EEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHCCCC
KVIIDPKSLVYLDGTELDYVKEGLNEGFEFNNPNAKGECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA