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The map label for this gene is iscS

Identifier: 218708629

GI number: 218708629

Start: 630560

End: 631774

Strand: Direct

Name: iscS

Synonym: VS_0607

Alternate gene names: 218708629

Gene position: 630560-631774 (Clockwise)

Preceding gene: 218708628

Following gene: 218708630

Centisome position: 19.11

GC content: 46.5

Gene sequence:

>1215_bases
ATGAAACTGCCTATTTACTTTGACTATTCAGCTACATGCCCAGTCGATCCACGTGTTGCTGAAAAAATGGTTCAGTGCAT
GACGATGGACGGTAACTTCGGTAACCCTGCATCTCGTTCACACCGTTACGGCTGGCAGGCAGAAGAATCAGTAGATAATG
CTCGTGAGCAAATTGCCGATCTACTAAATGCAGACCCACGTGAGATTGTTTTCACGTCAGGTGCTACAGAGTCAGATAAC
CTTGCTATCAAAGGTGCTGCGCACTTTTACGAGAAGAAAGGTAAGCACGTAATCACGTGCAAAACAGAACACAAAGCGGT
TCTTGATCCATGCCGCCAATTAGAGCGTGAAGGTTTTGAGGTGACATACCTTGAGCCAGAAGCAAACGGCATCATTGATC
TAGATAAGCTTCAAGCTGCAATGCGTGAAGATACTGTTCTAGTTTCTATCATGCACGTAAACAACGAGATTGGTGTAATC
CAAGATATCGGTGCAATCGGCGAATTATGTCGTGCACGCAAGATCATCTTCCACGTTGATGCAGCGCAGTCTGCGGGTAA
AATCCCACTAGACGTAAAAGAGATGAAAGTTGATCTTATCTCTATGTCTGCACACAAAGCATACGGCCCTAAAGGCATCG
GCGCTTTGTACGTTCGTCGTAAGCCACGTATTCGCCTTGAAGCGCAAATGCACGGTGGCGGTCATGAACGTGGTTTCCGT
TCTGGTACGCTTGCTACTCACCAAATCGTGGGTATGGGCGAAGCGTTTGCTATCGCTAAGCAAGACATGCAGAAAGATTA
CGATCACGCACTAGCACTTCGTGAGCGCCTATTGAAAGGTGTTCAAGATCTAGAAGCAGTAACCGTAAACGGCGACTTAG
ACCAACGTGTACCACACAACCTAAACGTGAGCTTCGCTTTCGTTGAAGGTGAGTCTCTGCTTATGTCTCTAAAAGACCTA
GCGGTATCATCAGGTTCTGCATGTACATCAGCAAGCCTAGAGCCTTCATACGTTCTACGTGCTCTTGGTCTGAACGATGA
ACTGGCACACAGCTCAGTACGTTTCTCATTCGGCCGTTTCACAACGGAAGAAGAAATCGACTACGCGATTGCACAAATCC
GTGTAGCGGTAAACAAATTACGCGACATGTCTCCTCTATGGGATATGTATAAAGAAGGGATTGATTTGAACACTGTTGAG
TGGGCTCATCACTAA

Upstream 100 bases:

>100_bases
TGGTTTTGCACATAAAAATACGAGCACTACAACGATTAGTGCAGCACCTCACGGTGTTAATGCCCGCTCTTAGCGGTCAG
TTTTTACATTGGAGTAGAAA

Downstream 100 bases:

>100_bases
TCTCACGGAAATAGAGGATTCGAGGTAACTATCATGGCATATAGCGAAAAAGTAATTGATCACTACGAAAACCCACGTAA
TGTAGGTTCGTTTGATAAAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYFDYSATCPVDPRVAEKMVQCMTMDGNFGNPASRSHRYGWQAEESVDNAREQIADLLNADPREIVFTSGATESDN
LAIKGAAHFYEKKGKHVITCKTEHKAVLDPCRQLEREGFEVTYLEPEANGIIDLDKLQAAMREDTVLVSIMHVNNEIGVI
QDIGAIGELCRARKIIFHVDAAQSAGKIPLDVKEMKVDLISMSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLATHQIVGMGEAFAIAKQDMQKDYDHALALRERLLKGVQDLEAVTVNGDLDQRVPHNLNVSFAFVEGESLLMSLKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSVRFSFGRFTTEEEIDYAIAQIRVAVNKLRDMSPLWDMYKEGIDLNTVE
WAHH

Sequences:

>Translated_404_residues
MKLPIYFDYSATCPVDPRVAEKMVQCMTMDGNFGNPASRSHRYGWQAEESVDNAREQIADLLNADPREIVFTSGATESDN
LAIKGAAHFYEKKGKHVITCKTEHKAVLDPCRQLEREGFEVTYLEPEANGIIDLDKLQAAMREDTVLVSIMHVNNEIGVI
QDIGAIGELCRARKIIFHVDAAQSAGKIPLDVKEMKVDLISMSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLATHQIVGMGEAFAIAKQDMQKDYDHALALRERLLKGVQDLEAVTVNGDLDQRVPHNLNVSFAFVEGESLLMSLKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSVRFSFGRFTTEEEIDYAIAQIRVAVNKLRDMSPLWDMYKEGIDLNTVE
WAHH
>Mature_404_residues
MKLPIYFDYSATCPVDPRVAEKMVQCMTMDGNFGNPASRSHRYGWQAEESVDNAREQIADLLNADPREIVFTSGATESDN
LAIKGAAHFYEKKGKHVITCKTEHKAVLDPCRQLEREGFEVTYLEPEANGIIDLDKLQAAMREDTVLVSIMHVNNEIGVI
QDIGAIGELCRARKIIFHVDAAQSAGKIPLDVKEMKVDLISMSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLATHQIVGMGEAFAIAKQDMQKDYDHALALRERLLKGVQDLEAVTVNGDLDQRVPHNLNVSFAFVEGESLLMSLKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSVRFSFGRFTTEEEIDYAIAQIRVAVNKLRDMSPLWDMYKEGIDLNTVE
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=57.4626865671642, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=32.5358851674641, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=76.4851485148515, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=355, Percent_Identity=25.3521126760563, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789175, Length=245, Percent_Identity=29.3877551020408, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=58.560794044665, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=33.9393939393939, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.1072319201995, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI20129463, Length=403, Percent_Identity=57.5682382133995, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_VIBSL (B7VJS6)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416250.1
- ProteinModelPortal:   B7VJS6
- SMR:   B7VJS6
- GeneID:   7160227
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0607
- HOGENOM:   HBG635316
- OMA:   DMSPLWD
- ProtClustDB:   PRK14012
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44857; Mature: 44857

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYFDYSATCPVDPRVAEKMVQCMTMDGNFGNPASRSHRYGWQAEESVDNAREQIAD
CCCEEEECCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGAAHFYEKKGKHVITCKTEHKAVLDPCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCE
VTYLEPEANGIIDLDKLQAAMREDTVLVSIMHVNNEIGVIQDIGAIGELCRARKIIFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEEEE
AAQSAGKIPLDVKEMKVDLISMSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCCCCCHHHHHEEHHEECHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLATHQIVGMGEAFAIAKQDMQKDYDHALALRERLLKGVQDLEAVTVNGDLDQRVPHN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHCCCCC
LNVSFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSVRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCHHEEECCCC
TTEEEIDYAIAQIRVAVNKLRDMSPLWDMYKEGIDLNTVEWAHH
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYFDYSATCPVDPRVAEKMVQCMTMDGNFGNPASRSHRYGWQAEESVDNAREQIAD
CCCEEEECCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGAAHFYEKKGKHVITCKTEHKAVLDPCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCE
VTYLEPEANGIIDLDKLQAAMREDTVLVSIMHVNNEIGVIQDIGAIGELCRARKIIFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEEEE
AAQSAGKIPLDVKEMKVDLISMSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCCCCCHHHHHEEHHEECHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLATHQIVGMGEAFAIAKQDMQKDYDHALALRERLLKGVQDLEAVTVNGDLDQRVPHN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHCCCCC
LNVSFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSVRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCHHEEECCCC
TTEEEIDYAIAQIRVAVNKLRDMSPLWDMYKEGIDLNTVEWAHH
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA