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The map label for this gene is folK [H]

Identifier: 218708442

GI number: 218708442

Start: 410724

End: 411206

Strand: Direct

Name: folK [H]

Synonym: VS_0405

Alternate gene names: 218708442

Gene position: 410724-411206 (Clockwise)

Preceding gene: 218708441

Following gene: 218708443

Centisome position: 12.45

GC content: 42.86

Gene sequence:

>483_bases
ATGACAATAGCCTATGTTGGTGTTGGCACGAACATAGACCGCGACAAGCATGCAAGGGTGGCATGGACAGAGCTTCAATC
ATTAGGGACTAACCTTAAATGCTCTAAGATCTATCACTGTGAGCCTGTGGGGTTTAAAAGCCACTCGTTTTATAACTTTG
TGGTTGAGATCGATACATCACTCTCATTGACTGAATTTTCACAGCACCTGCGTGAAATTGAGTTTAAATGGGGTCGCCCT
CAAGATGCCCATAAGCTTCAAGATCGAAAGCTCGATCTTGATATTGTGCTGTTTGGAGAGGTGGTATCAGAGAGCGATCC
AGAATTACCACGCAGTGATATCTATAAATATCCTTTTGTAACACAACCACTTTATGATCTTTGTCCTGCGAGAGTGATCC
CGCAAGATGGAAGAACCGTCAGTGAAATATGGCAGAAGATGCAGCAATTAGATACGCTCTCTGTCGTTGATATAACACTA
TAA

Upstream 100 bases:

>100_bases
TGACTCAGTTCTCAGTGCCTTGGATTAAAATCCGTTTAGCGAAGCCTGGCGCAGTACCACAAGCAAAAGCCGTGGGTGTG
ATCATCGAACGAGGCCAAGC

Downstream 100 bases:

>100_bases
TTTTTTAGGGTAAGTAATGAGTTATTTTGAAGCGTTTATGTTGGCGTTGGTGCAAGGCTTTACTGAGTTTTTACCTATTT
CCAGCTCTGCACACTTAATC

Product: 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTIAYVGVGTNIDRDKHARVAWTELQSLGTNLKCSKIYHCEPVGFKSHSFYNFVVEIDTSLSLTEFSQHLREIEFKWGRP
QDAHKLQDRKLDLDIVLFGEVVSESDPELPRSDIYKYPFVTQPLYDLCPARVIPQDGRTVSEIWQKMQQLDTLSVVDITL

Sequences:

>Translated_160_residues
MTIAYVGVGTNIDRDKHARVAWTELQSLGTNLKCSKIYHCEPVGFKSHSFYNFVVEIDTSLSLTEFSQHLREIEFKWGRP
QDAHKLQDRKLDLDIVLFGEVVSESDPELPRSDIYKYPFVTQPLYDLCPARVIPQDGRTVSEIWQKMQQLDTLSVVDITL
>Mature_159_residues
TIAYVGVGTNIDRDKHARVAWTELQSLGTNLKCSKIYHCEPVGFKSHSFYNFVVEIDTSLSLTEFSQHLREIEFKWGRPQ
DAHKLQDRKLDLDIVLFGEVVSESDPELPRSDIYKYPFVTQPLYDLCPARVIPQDGRTVSEIWQKMQQLDTLSVVDITL

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=150, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18447; Mature: 18316

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAYVGVGTNIDRDKHARVAWTELQSLGTNLKCSKIYHCEPVGFKSHSFYNFVVEIDTS
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEECEEEECCCCCCCCCCEEEEEEEECCC
LSLTEFSQHLREIEFKWGRPQDAHKLQDRKLDLDIVLFGEVVSESDPELPRSDIYKYPFV
CCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEHHHCCCCCCCCHHHCCCCCCC
TQPLYDLCPARVIPQDGRTVSEIWQKMQQLDTLSVVDITL
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure 
TIAYVGVGTNIDRDKHARVAWTELQSLGTNLKCSKIYHCEPVGFKSHSFYNFVVEIDTS
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEECEEEECCCCCCCCCCEEEEEEEECCC
LSLTEFSQHLREIEFKWGRPQDAHKLQDRKLDLDIVLFGEVVSESDPELPRSDIYKYPFV
CCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEHHHCCCCCCCCHHHCCCCCCC
TQPLYDLCPARVIPQDGRTVSEIWQKMQQLDTLSVVDITL
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]