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The map label for this gene is tapB [H]

Identifier: 218708371

GI number: 218708371

Start: 325365

End: 327089

Strand: Direct

Name: tapB [H]

Synonym: VS_0331

Alternate gene names: 218708371

Gene position: 325365-327089 (Clockwise)

Preceding gene: 218708370

Following gene: 218708372

Centisome position: 9.86

GC content: 45.8

Gene sequence:

>1725_bases
ATGCAAATTAGATTAAGAAAAAGGCTCGGTGATTTGCTCGTAGAAGAGGGCATCATTACTGAATATCAAGTTGAACAAGC
TCTGGCCGCTCAAAAAAGGACAGGCCGTAAGCTTGGTGATGCACTGATCGAGCTTGGCTTTTTATCAGAGCAGCAAATGC
TCAGTTTCTTGTCGCAACAGCTTGCTATTCCTCTTATTGATATTAGCCGAGCTAATGTTGACGTCGAAGCTGTGCAGCTT
TTACCTGAGGTTCATGCTCGCCGTCTTCGTGCTTTGATCATTGGGCGTCAAGGTGACACATTACGTGTTGCAATGAGTGA
TCCTGCCGATCTGTTTGCCCAAGAATCTTTGCTGAGTCAACTAGGCGACTACGCACTCGAATTTGTCGTCGCACAAGAGA
GGCAATTAGTTGATGGTTTCGACCGTTATTACCGAAGAACCAAAGAGATTGCCTCATTTGCTGAGCAGCTTCATGCTGAG
CATAAAGTTAATGAAGCCTTTGATTTTGATATCGCAGAAGAAGACAGCGACGAAGTTACAGTCGTCAAGCTAGTAAACTC
GCTGTTTGAAGATGCTATTCAAGTTGGTGCATCTGATATTCATATTGAACCAGATTCAAACGTATTACGTTTGCGCCAGC
GTATTGATGGTGTTCTGCATGAAACGTTGTTGAATGAAGTGAATATTGCTTCTGCACTGGTTTTGCGCTTGAAGTTGATG
GCTAACCTCGACATTTCAGAGAAACGACTGCCTCAAGATGGTCGCTTTAATATTCGTGTTAAAGGTCAATCGGTTGATAT
TCGTATGTCGACTATGCCAGTACAGCACGGTGAGTCAGTGGTTATGCGTTTGCTTAATCAATCCGCAGGCCTACGTAAAT
TGGAGTTGTCTGGCATTCCGAGTGATTTGCTCGTTCGTCTTCGTCATCAATTACGCCGCCCACATGGCATGATCCTCGTT
ACCGGGCCTACGGGCTCAGGTAAAACAACCACTCTATACGGTGCGCTAAGTGAATTAAACGAACCGGGTAAAAAGATCAT
TACCGCTGAAGACCCTGTAGAATACCGTCTTCCTCGTGTGAATCAGGTTCAGGTAAACCCTAAGATTAACCTCAACTTCT
CAACGATTTTGAGAACCTTCCTACGTCAGGACCCGGATATCATTCTTATTGGTGAGATGCGAGATCATGAAACCGTTGAA
ATTGGTTTGAGAGCGGCACTTACTGGTCACTTAGTGTTAAGTACTCTGCATACCAACGATGCAGTAGACAGTGCATTGCG
TATGATGGACATGGGGGCTCCGGGTTATCTGGTAGCAAGTGCTGTTCGAGCCGTGGTCGCACAGCGGTTAGTTCGTAAGG
TATGTACTGATTGCAAGGTTGATGATGAGCTGGATGAAGCGCGTAAGCAATGGTTGAGTGTTCGTTTCCCAAACCAGCTA
GGTACGCCATTTATGAAAGGGCGTGGTTGTCAAAATTGTAATTTGACAGGCTACAGTGGTCGTGTTGGTGTGTTTGAAAT
GTTGGAGCTCGATCAGCCTATGATGGATGCGCTTAGAGCCAATGATGCCGTTGGTTTTGCTCAAACGGCAAGGCAGTCTG
AAAACTACAAACCGCTGTTAGCTTCTGCGATGGAATTGGCCATGCAGGGTGTTGTGAGCTTCGATGAGGTAATGAACCTT
GGCGAAGGCGATGCTTCTGGTGCCACTGATCCAATTTATCTGTAG

Upstream 100 bases:

>100_bases
TCATACCAAGGAGCATTGGTTAGAGTTGGGATCTCATCTCAGTCGCAAGGCTTTGTACGTGATCGTTTAAAAATACTCAA
TGCTTTAGAGGAGAGTAGCG

Downstream 100 bases:

>100_bases
GGGTAGAGTGAAATGCCAACTTATCGTTATGTAGGTCGCAGTTCTGATGGCAGTCAAGTGACAGGTCAGTTGGATGCGAA
TAACGAAGATCTTGCTGCTG

Product: MSHA biogenesis protein MshE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MQIRLRKRLGDLLVEEGIITEYQVEQALAAQKRTGRKLGDALIELGFLSEQQMLSFLSQQLAIPLIDISRANVDVEAVQL
LPEVHARRLRALIIGRQGDTLRVAMSDPADLFAQESLLSQLGDYALEFVVAQERQLVDGFDRYYRRTKEIASFAEQLHAE
HKVNEAFDFDIAEEDSDEVTVVKLVNSLFEDAIQVGASDIHIEPDSNVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPVQHGESVVMRLLNQSAGLRKLELSGIPSDLLVRLRHQLRRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRVNQVQVNPKINLNFSTILRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMMDMGAPGYLVASAVRAVVAQRLVRKVCTDCKVDDELDEARKQWLSVRFPNQL
GTPFMKGRGCQNCNLTGYSGRVGVFEMLELDQPMMDALRANDAVGFAQTARQSENYKPLLASAMELAMQGVVSFDEVMNL
GEGDASGATDPIYL

Sequences:

>Translated_574_residues
MQIRLRKRLGDLLVEEGIITEYQVEQALAAQKRTGRKLGDALIELGFLSEQQMLSFLSQQLAIPLIDISRANVDVEAVQL
LPEVHARRLRALIIGRQGDTLRVAMSDPADLFAQESLLSQLGDYALEFVVAQERQLVDGFDRYYRRTKEIASFAEQLHAE
HKVNEAFDFDIAEEDSDEVTVVKLVNSLFEDAIQVGASDIHIEPDSNVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPVQHGESVVMRLLNQSAGLRKLELSGIPSDLLVRLRHQLRRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRVNQVQVNPKINLNFSTILRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMMDMGAPGYLVASAVRAVVAQRLVRKVCTDCKVDDELDEARKQWLSVRFPNQL
GTPFMKGRGCQNCNLTGYSGRVGVFEMLELDQPMMDALRANDAVGFAQTARQSENYKPLLASAMELAMQGVVSFDEVMNL
GEGDASGATDPIYL
>Mature_574_residues
MQIRLRKRLGDLLVEEGIITEYQVEQALAAQKRTGRKLGDALIELGFLSEQQMLSFLSQQLAIPLIDISRANVDVEAVQL
LPEVHARRLRALIIGRQGDTLRVAMSDPADLFAQESLLSQLGDYALEFVVAQERQLVDGFDRYYRRTKEIASFAEQLHAE
HKVNEAFDFDIAEEDSDEVTVVKLVNSLFEDAIQVGASDIHIEPDSNVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPVQHGESVVMRLLNQSAGLRKLELSGIPSDLLVRLRHQLRRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRVNQVQVNPKINLNFSTILRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMMDMGAPGYLVASAVRAVVAQRLVRKVCTDCKVDDELDEARKQWLSVRFPNQL
GTPFMKGRGCQNCNLTGYSGRVGVFEMLELDQPMMDALRANDAVGFAQTARQSENYKPLLASAMELAMQGVVSFDEVMNL
GEGDASGATDPIYL

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=40.9756097560976, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1786296, Length=433, Percent_Identity=37.6443418013857, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI87082188, Length=213, Percent_Identity=38.4976525821596, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 63911; Mature: 63911

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRLRKRLGDLLVEEGIITEYQVEQALAAQKRTGRKLGDALIELGFLSEQQMLSFLSQQ
CCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAIPLIDISRANVDVEAVQLLPEVHARRLRALIIGRQGDTLRVAMSDPADLFAQESLLSQ
HCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHH
LGDYALEFVVAQERQLVDGFDRYYRRTKEIASFAEQLHAEHKVNEAFDFDIAEEDSDEVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCH
VVKLVNSLFEDAIQVGASDIHIEPDSNVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPVQHGESVVMRLLNQSAGLRKLELSGIP
HCCCCCHHHCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCC
SDLLVRLRHQLRRPHGMILVTGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRV
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHCCCCC
NQVQVNPKINLNFSTILRTFLRQDPDIILIGEMRDHETVEIGLRAALTGHLVLSTLHTND
CEEEECCEEECCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHH
AVDSALRMMDMGAPGYLVASAVRAVVAQRLVRKVCTDCKVDDELDEARKQWLSVRFPNQL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEECCHHH
GTPFMKGRGCQNCNLTGYSGRVGVFEMLELDQPMMDALRANDAVGFAQTARQSENYKPLL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHH
ASAMELAMQGVVSFDEVMNLGEGDASGATDPIYL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIRLRKRLGDLLVEEGIITEYQVEQALAAQKRTGRKLGDALIELGFLSEQQMLSFLSQQ
CCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAIPLIDISRANVDVEAVQLLPEVHARRLRALIIGRQGDTLRVAMSDPADLFAQESLLSQ
HCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHH
LGDYALEFVVAQERQLVDGFDRYYRRTKEIASFAEQLHAEHKVNEAFDFDIAEEDSDEVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCH
VVKLVNSLFEDAIQVGASDIHIEPDSNVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPVQHGESVVMRLLNQSAGLRKLELSGIP
HCCCCCHHHCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCC
SDLLVRLRHQLRRPHGMILVTGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRV
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHCCCCC
NQVQVNPKINLNFSTILRTFLRQDPDIILIGEMRDHETVEIGLRAALTGHLVLSTLHTND
CEEEECCEEECCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHH
AVDSALRMMDMGAPGYLVASAVRAVVAQRLVRKVCTDCKVDDELDEARKQWLSVRFPNQL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEECCHHH
GTPFMKGRGCQNCNLTGYSGRVGVFEMLELDQPMMDALRANDAVGFAQTARQSENYKPLL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHH
ASAMELAMQGVVSFDEVMNLGEGDASGATDPIYL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]