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The map label for this gene is rffH [H]

Identifier: 218708290

GI number: 218708290

Start: 222749

End: 223645

Strand: Direct

Name: rffH [H]

Synonym: VS_0228

Alternate gene names: 218708290

Gene position: 222749-223645 (Clockwise)

Preceding gene: 218708289

Following gene: 218708291

Centisome position: 6.75

GC content: 38.8

Gene sequence:

>897_bases
ATGACTAAAGCTTCAGTTTCAAAATATAAAGGCATTATTCTAGCCGGTGGTTCAGGTAGTCGTTTGCATCCTATTACGTT
GGGTGCCTCGAAACAGTTATTACCAATTTACGATAAGCCAATGATTTTCTATCCGCTATCAGTTTTGATGTTGGCGGGCA
TTAAAGATATTCTAATTATCTCAACACCAGAAGACTTGCCTAATTTTGAGCGTATGCTGGGCGACGGTAGTCAATTTGGC
GTTCAAATTGTTTATAAAGAGCAACCAACTCCAGACGGGTTAGCACAAGCATTTATCATAGGCGAAGAATTTATTGGCAA
TGATAACGTAGCTTTAGTATTAGGTGATAACATCTTCTATGGTCAACATTTTTCAGATAAGCTACTAGCTGCTGCAAAAG
TTGAAAAAGGTGCTTCAGTATTTGGTTATCATGTTTCTGATCCTGACCGTTTTGGCGTAGTAGAGTTTGATGAAAATGGT
AAGGCGCTAAGTATTGAAGAGAAGCCTGAACACCCCAAATCAAACTATGCTGTTACAGGCCTGTACTTTTATGACAATGA
TGTAGTTGAAATCGCAAAAAGCATTAAACCATCTCACCGTGGGGAATTAGAAATTACCGATGTTAACATCGCTTATTTAG
ATCGTGGTGATTTGAATGTTGAGCTGTTAGGTCGCGGTTTTGCTTGGCTTGATACGGGTACGCATGACTCACTACTTGAA
GCTAGTCAATTCGTACAAACTGTTGAGCATCGTCAAGGCTTTAAAGTCGCTTGTTTAGAAGAGATTGCTTACGTGCAAGG
TTGGTTAAGTAAAGATGAACTACTTGTTCAAGCAGAAAAACTGAAGAAAACAGGTTACGGTCAGTACTTATTTAATATCG
CTAATGGAGTGATTTAA

Upstream 100 bases:

>100_bases
GAATTAGAAAGACAGTTGAATGGTATTTAAGTAATGAAAATTGGTGGAAGCGTGTATTAAATGGGGAATACCAACTAGAA
CGTATCGGAGAGATGTAACC

Downstream 100 bases:

>100_bases
TGAAATTTATCGATACTGCTATTTCTGATGTGAAAATTGTTGAAGTGAGTGTATTTGGTGATGAACGAGGTTTCTTTATG
GAAACATTTCGTGCAGATGA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTKASVSKYKGIILAGGSGSRLHPITLGASKQLLPIYDKPMIFYPLSVLMLAGIKDILIISTPEDLPNFERMLGDGSQFG
VQIVYKEQPTPDGLAQAFIIGEEFIGNDNVALVLGDNIFYGQHFSDKLLAAAKVEKGASVFGYHVSDPDRFGVVEFDENG
KALSIEEKPEHPKSNYAVTGLYFYDNDVVEIAKSIKPSHRGELEITDVNIAYLDRGDLNVELLGRGFAWLDTGTHDSLLE
ASQFVQTVEHRQGFKVACLEEIAYVQGWLSKDELLVQAEKLKKTGYGQYLFNIANGVI

Sequences:

>Translated_298_residues
MTKASVSKYKGIILAGGSGSRLHPITLGASKQLLPIYDKPMIFYPLSVLMLAGIKDILIISTPEDLPNFERMLGDGSQFG
VQIVYKEQPTPDGLAQAFIIGEEFIGNDNVALVLGDNIFYGQHFSDKLLAAAKVEKGASVFGYHVSDPDRFGVVEFDENG
KALSIEEKPEHPKSNYAVTGLYFYDNDVVEIAKSIKPSHRGELEITDVNIAYLDRGDLNVELLGRGFAWLDTGTHDSLLE
ASQFVQTVEHRQGFKVACLEEIAYVQGWLSKDELLVQAEKLKKTGYGQYLFNIANGVI
>Mature_297_residues
TKASVSKYKGIILAGGSGSRLHPITLGASKQLLPIYDKPMIFYPLSVLMLAGIKDILIISTPEDLPNFERMLGDGSQFGV
QIVYKEQPTPDGLAQAFIIGEEFIGNDNVALVLGDNIFYGQHFSDKLLAAAKVEKGASVFGYHVSDPDRFGVVEFDENGK
ALSIEEKPEHPKSNYAVTGLYFYDNDVVEIAKSIKPSHRGELEITDVNIAYLDRGDLNVELLGRGFAWLDTGTHDSLLEA
SQFVQTVEHRQGFKVACLEEIAYVQGWLSKDELLVQAEKLKKTGYGQYLFNIANGVI

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=284, Percent_Identity=70.7746478873239, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1788351, Length=285, Percent_Identity=66.3157894736842, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1787488, Length=234, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133931050, Length=183, Percent_Identity=25.6830601092896, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=253, Percent_Identity=25.296442687747, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355443, Length=253, Percent_Identity=24.1106719367589, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644084, Length=253, Percent_Identity=24.1106719367589, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32838; Mature: 32707

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKASVSKYKGIILAGGSGSRLHPITLGASKQLLPIYDKPMIFYPLSVLMLAGIKDILII
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEE
STPEDLPNFERMLGDGSQFGVQIVYKEQPTPDGLAQAFIIGEEFIGNDNVALVLGDNIFY
ECCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEEECHHHCCCCCEEEEEECCEEE
GQHFSDKLLAAAKVEKGASVFGYHVSDPDRFGVVEFDENGKALSIEEKPEHPKSNYAVTG
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEE
LYFYDNDVVEIAKSIKPSHRGELEITDVNIAYLDRGDLNVELLGRGFAWLDTGTHDSLLE
EEEECCHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCEEEEECCCCHHHHH
ASQFVQTVEHRQGFKVACLEEIAYVQGWLSKDELLVQAEKLKKTGYGQYLFNIANGVI
HHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TKASVSKYKGIILAGGSGSRLHPITLGASKQLLPIYDKPMIFYPLSVLMLAGIKDILII
CCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEE
STPEDLPNFERMLGDGSQFGVQIVYKEQPTPDGLAQAFIIGEEFIGNDNVALVLGDNIFY
ECCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEEECHHHCCCCCEEEEEECCEEE
GQHFSDKLLAAAKVEKGASVFGYHVSDPDRFGVVEFDENGKALSIEEKPEHPKSNYAVTG
CCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEE
LYFYDNDVVEIAKSIKPSHRGELEITDVNIAYLDRGDLNVELLGRGFAWLDTGTHDSLLE
EEEECCHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCEEEEECCCCHHHHH
ASQFVQTVEHRQGFKVACLEEIAYVQGWLSKDELLVQAEKLKKTGYGQYLFNIANGVI
HHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]