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The map label for this gene is rffG [H]

Identifier: 218708289

GI number: 218708289

Start: 221670

End: 222746

Strand: Direct

Name: rffG [H]

Synonym: VS_0227

Alternate gene names: 218708289

Gene position: 221670-222746 (Clockwise)

Preceding gene: 218708288

Following gene: 218708290

Centisome position: 6.72

GC content: 39.74

Gene sequence:

>1077_bases
ATGATTTTCATTTCCGGCGGTGCTGGCTTTATTGGCAGTGCCGTAGTGCGCCATATCATCACAAACACCACTGAGTCAAT
TGTTAATGTAGATAAACTTACCTACGCTGGTAATGTTGAATCTATCGCTCTTATTTCCGGGAGTGACCGATATCATTTTG
TACAAGCTGATATTAGTGATGCCGATGCAATGGCTGAGGTATTTGAAAAATATCAACCAACGGCTGTGATGCACTTGGCT
GCTGAATCTCATGTCGATCGTTCAATTGATGGCCCAGCAGATTTCATGCAAACTAATATAATGGGTACGTTCACTCTGCT
AGAAGCTGCACGTGTTTATTGGTCTACTTTACCGCAAGATAAAAGAGAAACTTTCCGCTTTCATCACATAAGTACTGATG
AAGTGTACGGTGATTTGGAAGGGACTGATGAGCTGTTTACAGAAGAAACATCCTATGAGCCCAGCTCTCCGTATTCAGCA
TCTAAAGCCTCAAGCGACCACTTAGTTCGTGCTTGGAATCGAACTTACGGTTTACCAGTTATTGTCACTAACTGTTCAAA
TAACTATGGTCCTTATCATTTTCCTGAAAAATTGATTCCACACGTTATTTTAAATGCCTTAGATGCAAAACCATTACCTA
TTTACGGTGATGGCAGTCAGATTCGTGATTGGCTATATGTAGAAGACCATGCTCGAGCTCTTTATAAAGTAGTAACCGAG
GGAGTTGTGGGCGAGACCTATAATATTGGTGGTCATAACGAGAAGAAAAATATTGAAGTAGTTGAAACACTTTGTACTAT
TCTTGATGAATTAAAACCTATATCTGATAACCCAGCTTTTACTTCTTCCTCAATAGTCAATTATAACGAACTTATTACAT
TTGTTAAAGATCGCCCAGGTCACGATGTCCGCTATGCAATCGACGCTTCAAAAATTGAAAAAGAACTAGGCTGGGTGCCA
GAGGAAAGCTTTGAGACAGGAATTAGAAAGACAGTTGAATGGTATTTAAGTAATGAAAATTGGTGGAAGCGTGTATTAAA
TGGGGAATACCAACTAGAACGTATCGGAGAGATGTAA

Upstream 100 bases:

>100_bases
TGTCGGCTCTTGGAAGCAACTCGTAGAAAAATTTATATCGATAGCTAATGAGAAATAACTATTTGTCTTTTATTTTGACT
GTTTATATTTGGAACTAATT

Downstream 100 bases:

>100_bases
CCATGACTAAAGCTTCAGTTTCAAAATATAAAGGCATTATTCTAGCCGGTGGTTCAGGTAGTCGTTTGCATCCTATTACG
TTGGGTGCCTCGAAACAGTT

Product: dTDP-D-glucose-4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIFISGGAGFIGSAVVRHIITNTTESIVNVDKLTYAGNVESIALISGSDRYHFVQADISDADAMAEVFEKYQPTAVMHLA
AESHVDRSIDGPADFMQTNIMGTFTLLEAARVYWSTLPQDKRETFRFHHISTDEVYGDLEGTDELFTEETSYEPSSPYSA
SKASSDHLVRAWNRTYGLPVIVTNCSNNYGPYHFPEKLIPHVILNALDAKPLPIYGDGSQIRDWLYVEDHARALYKVVTE
GVVGETYNIGGHNEKKNIEVVETLCTILDELKPISDNPAFTSSSIVNYNELITFVKDRPGHDVRYAIDASKIEKELGWVP
EESFETGIRKTVEWYLSNENWWKRVLNGEYQLERIGEM

Sequences:

>Translated_358_residues
MIFISGGAGFIGSAVVRHIITNTTESIVNVDKLTYAGNVESIALISGSDRYHFVQADISDADAMAEVFEKYQPTAVMHLA
AESHVDRSIDGPADFMQTNIMGTFTLLEAARVYWSTLPQDKRETFRFHHISTDEVYGDLEGTDELFTEETSYEPSSPYSA
SKASSDHLVRAWNRTYGLPVIVTNCSNNYGPYHFPEKLIPHVILNALDAKPLPIYGDGSQIRDWLYVEDHARALYKVVTE
GVVGETYNIGGHNEKKNIEVVETLCTILDELKPISDNPAFTSSSIVNYNELITFVKDRPGHDVRYAIDASKIEKELGWVP
EESFETGIRKTVEWYLSNENWWKRVLNGEYQLERIGEM
>Mature_358_residues
MIFISGGAGFIGSAVVRHIITNTTESIVNVDKLTYAGNVESIALISGSDRYHFVQADISDADAMAEVFEKYQPTAVMHLA
AESHVDRSIDGPADFMQTNIMGTFTLLEAARVYWSTLPQDKRETFRFHHISTDEVYGDLEGTDELFTEETSYEPSSPYSA
SKASSDHLVRAWNRTYGLPVIVTNCSNNYGPYHFPEKLIPHVILNALDAKPLPIYGDGSQIRDWLYVEDHARALYKVVTE
GVVGETYNIGGHNEKKNIEVVETLCTILDELKPISDNPAFTSSSIVNYNELITFVKDRPGHDVRYAIDASKIEKELGWVP
EESFETGIRKTVEWYLSNENWWKRVLNGEYQLERIGEM

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=33.9130434782609, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=24.7787610619469, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4504031, Length=351, Percent_Identity=22.2222222222222, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=68.4507042253521, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=70.6703910614525, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=24.7191011235955, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788365, Length=355, Percent_Identity=23.0985915492958, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788366, Length=247, Percent_Identity=24.6963562753036, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=35.7771260997067, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=33.6309523809524, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=351, Percent_Identity=25.6410256410256, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=376, Percent_Identity=24.7340425531915, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=378, Percent_Identity=24.6031746031746, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=196, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=196, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=196, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=196, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=196, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=199, Percent_Identity=25.1256281407035, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=299, Percent_Identity=26.4214046822742, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19923002, Length=376, Percent_Identity=25.2659574468085, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=25.6637168141593, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40362; Mature: 40362

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFISGGAGFIGSAVVRHIITNTTESIVNVDKLTYAGNVESIALISGSDRYHFVQADISD
CEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCEEEEEECCCCEEEEECCCCC
ADAMAEVFEKYQPTAVMHLAAESHVDRSIDGPADFMQTNIMGTFTLLEAARVYWSTLPQD
HHHHHHHHHHHCCHHEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRETFRFHHISTDEVYGDLEGTDELFTEETSYEPSSPYSASKASSDHLVRAWNRTYGLPV
HHHHEEEEECCHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
IVTNCSNNYGPYHFPEKLIPHVILNALDAKPLPIYGDGSQIRDWLYVEDHARALYKVVTE
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHEEEEHHHHHHHHHHHHH
GVVGETYNIGGHNEKKNIEVVETLCTILDELKPISDNPAFTSSSIVNYNELITFVKDRPG
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
HDVRYAIDASKIEKELGWVPEESFETGIRKTVEWYLSNENWWKRVLNGEYQLERIGEM
CCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MIFISGGAGFIGSAVVRHIITNTTESIVNVDKLTYAGNVESIALISGSDRYHFVQADISD
CEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCEEEEEECCCCEEEEECCCCC
ADAMAEVFEKYQPTAVMHLAAESHVDRSIDGPADFMQTNIMGTFTLLEAARVYWSTLPQD
HHHHHHHHHHHCCHHEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRETFRFHHISTDEVYGDLEGTDELFTEETSYEPSSPYSASKASSDHLVRAWNRTYGLPV
HHHHEEEEECCHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
IVTNCSNNYGPYHFPEKLIPHVILNALDAKPLPIYGDGSQIRDWLYVEDHARALYKVVTE
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHEEEEHHHHHHHHHHHHH
GVVGETYNIGGHNEKKNIEVVETLCTILDELKPISDNPAFTSSSIVNYNELITFVKDRPG
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
HDVRYAIDASKIEKELGWVPEESFETGIRKTVEWYLSNENWWKRVLNGEYQLERIGEM
CCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]