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The map label for this gene is htrB [H]

Identifier: 218708242

GI number: 218708242

Start: 176154

End: 177143

Strand: Reverse

Name: htrB [H]

Synonym: VS_0179

Alternate gene names: 218708242

Gene position: 177143-176154 (Counterclockwise)

Preceding gene: 218708243

Following gene: 218708239

Centisome position: 5.37

GC content: 45.86

Gene sequence:

>990_bases
GTGATGGTTATGACGACGAAAAACTCTCCTGCAAGTAGCACGGTACAACACGTTATTAATAAGCCACCTTTTACCCTAGC
ACTACTGCACCCTAAATATTGGGGAGTTTGGTTTGGCTTTGGGTTACTGGCTTTTATAGTCAACATTCTACCTTACCGAG
TATTACTACTATTAGGCCGTTCGTTAGGTGCTCTTGGTGCCCGCTATGGAAAAAAACGCGTAGCGGTAGCGACACGTAAC
TTGGAATTAGCCTTCCCCGATAAGCCAGCAGATGAAGTCGCGGCAATGGTCAGCGAGAATTTTAAAAATACTGGTATGGC
ATTGGTTGAAACCGGTATTACGTGGTTTTGGCCAACATGGCGCTTCAAGCGCATCCTAGTTGATAAAGATACCGAAGTTC
TTCGTAGTCATCAGGCTAACGGTAAAGGCGTTCTACTATGTTGTGTTCATGCCTTGAATCTAGAGATCACAGCCCGAGCA
ATGCCGATCTTGGGTATTCCTGGCCTAGGTGTTTATCGCCCGCATAACAACCCAGCTTATGAGTTCATTCAATACCGCGG
TCGAACTCAAAACGGCAACCGTCTAGTTCACCGTAAAGACGTAAAACGTATGATCCGAAGACTGCGACAAGGTGAGACCC
TTTTCTACCTCCCTGATCATGACTACGGTCGCAATAAATCTGTATTCGTACCTTTCTTTGCAGTAGAGGATGCAAGCACC
ACCACTGGAACCAGCATTTTGGCTTACACCAGTCGATGCGCGGTCGTTATTGGGTCTGGCTTTAGAAATGCCGACGGTAA
ATATGAAATCATGGCCGACGAATCAATCGAAGATAACTATCCGCAGAAAGATGAAAAAGCGGCAGCAGCGTATATGAACC
GCTATCTTGAGAAGATTATCTTAAGAGCCCCAGAGCAATGGATGTGGCTACACAAGCGCTTCAAAACCATGGAAGATCCA
GAAGAGGAAAAAGGCATTCGTTACAAGTAA

Upstream 100 bases:

>100_bases
ATCGATTTGTTCGCTCGGACTTCAAATATCAACCGACAGAAAACTTTGATGCTTATTGGGCTCTACTCAGCGCTCAGATT
AAGTAAACTCAATATCTTAA

Downstream 100 bases:

>100_bases
ACGAGCCTTGTTCTGTCCAATAAGATTTAAGTAAAAAAATGCCCGAAAGAGTTTCATCTCTCGGGCATTGTTCTTTTTTT
AAACTCTAAACAGAGGGAAC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MMVMTTKNSPASSTVQHVINKPPFTLALLHPKYWGVWFGFGLLAFIVNILPYRVLLLLGRSLGALGARYGKKRVAVATRN
LELAFPDKPADEVAAMVSENFKNTGMALVETGITWFWPTWRFKRILVDKDTEVLRSHQANGKGVLLCCVHALNLEITARA
MPILGIPGLGVYRPHNNPAYEFIQYRGRTQNGNRLVHRKDVKRMIRRLRQGETLFYLPDHDYGRNKSVFVPFFAVEDAST
TTGTSILAYTSRCAVVIGSGFRNADGKYEIMADESIEDNYPQKDEKAAAAYMNRYLEKIILRAPEQWMWLHKRFKTMEDP
EEEKGIRYK

Sequences:

>Translated_329_residues
MMVMTTKNSPASSTVQHVINKPPFTLALLHPKYWGVWFGFGLLAFIVNILPYRVLLLLGRSLGALGARYGKKRVAVATRN
LELAFPDKPADEVAAMVSENFKNTGMALVETGITWFWPTWRFKRILVDKDTEVLRSHQANGKGVLLCCVHALNLEITARA
MPILGIPGLGVYRPHNNPAYEFIQYRGRTQNGNRLVHRKDVKRMIRRLRQGETLFYLPDHDYGRNKSVFVPFFAVEDAST
TTGTSILAYTSRCAVVIGSGFRNADGKYEIMADESIEDNYPQKDEKAAAAYMNRYLEKIILRAPEQWMWLHKRFKTMEDP
EEEKGIRYK
>Mature_329_residues
MMVMTTKNSPASSTVQHVINKPPFTLALLHPKYWGVWFGFGLLAFIVNILPYRVLLLLGRSLGALGARYGKKRVAVATRN
LELAFPDKPADEVAAMVSENFKNTGMALVETGITWFWPTWRFKRILVDKDTEVLRSHQANGKGVLLCCVHALNLEITARA
MPILGIPGLGVYRPHNNPAYEFIQYRGRTQNGNRLVHRKDVKRMIRRLRQGETLFYLPDHDYGRNKSVFVPFFAVEDAST
TTGTSILAYTSRCAVVIGSGFRNADGKYEIMADESIEDNYPQKDEKAAAAYMNRYLEKIILRAPEQWMWLHKRFKTMEDP
EEEKGIRYK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=299, Percent_Identity=45.819397993311, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI87082094, Length=294, Percent_Identity=42.8571428571429, Blast_Score=250, Evalue=9e-68,
Organism=Escherichia coli, GI1788161, Length=298, Percent_Identity=28.5234899328859, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37563; Mature: 37563

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVMTTKNSPASSTVQHVINKPPFTLALLHPKYWGVWFGFGLLAFIVNILPYRVLLLLGR
CEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGALGARYGKKRVAVATRNLELAFPDKPADEVAAMVSENFKNTGMALVETGITWFWPTW
HHHHHHHHCCCCEEEEEEECEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEECCH
RFKRILVDKDTEVLRSHQANGKGVLLCCVHALNLEITARAMPILGIPGLGVYRPHNNPAY
HEEEEEECCCHHHHHHHCCCCCEEEEEEEEHHCEEEEEEECCEECCCCCCEECCCCCCHH
EFIQYRGRTQNGNRLVHRKDVKRMIRRLRQGETLFYLPDHDYGRNKSVFVPFFAVEDAST
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCC
TTGTSILAYTSRCAVVIGSGFRNADGKYEIMADESIEDNYPQKDEKAAAAYMNRYLEKII
CCCCCCEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LRAPEQWMWLHKRFKTMEDPEEEKGIRYK
HHCCHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure
MMVMTTKNSPASSTVQHVINKPPFTLALLHPKYWGVWFGFGLLAFIVNILPYRVLLLLGR
CEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGALGARYGKKRVAVATRNLELAFPDKPADEVAAMVSENFKNTGMALVETGITWFWPTW
HHHHHHHHCCCCEEEEEEECEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEECCH
RFKRILVDKDTEVLRSHQANGKGVLLCCVHALNLEITARAMPILGIPGLGVYRPHNNPAY
HEEEEEECCCHHHHHHHCCCCCEEEEEEEEHHCEEEEEEECCEECCCCCCEECCCCCCHH
EFIQYRGRTQNGNRLVHRKDVKRMIRRLRQGETLFYLPDHDYGRNKSVFVPFFAVEDAST
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCC
TTGTSILAYTSRCAVVIGSGFRNADGKYEIMADESIEDNYPQKDEKAAAAYMNRYLEKII
CCCCCCEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LRAPEQWMWLHKRFKTMEDPEEEKGIRYK
HHCCHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]