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The map label for this gene is epsJ [H]

Identifier: 218708205

GI number: 218708205

Start: 135617

End: 136420

Strand: Direct

Name: epsJ [H]

Synonym: VS_0141

Alternate gene names: 218708205

Gene position: 135617-136420 (Clockwise)

Preceding gene: 218708204

Following gene: 218708206

Centisome position: 4.11

GC content: 44.78

Gene sequence:

>804_bases
ATGTGGTCAATTAAAAGCGTGTGGTCAACTAAAAGCATGTGGTTAAGTAAAATCGTGTCGTTAGCGAAGAGCAAGTCGCT
AAGTAAAAGCATGTCGGCAAATAAGCATACGCCGCGTAAACAAGGTATATCTTCAAAAGGGAGAGGCTTTACTTTAATCG
AAGTTTTAGTTTCGATTGCCATCTTTTCCACGTTAAGCATGGCGGCTTATCAGGTGGTAAATCAGGTCCAACGAAGCAAC
GAACTCTCTATTGAGCGCAGTGCTCGTTTAAATCAACTGCAACGCAGTTTAGTCATTTTAGATAATGATTTTCGCCAGAT
GGCGGTACGACAATTTCGTACCAATGGTGAAGAAGCATCATCTAAGCTGATCCTAATGAAAGAGTATTTACTGGATTCCG
ACAGTGTTGGCATCATGTTTACTCGCCTTGGTTGGCACAACCCACAGCAGCAGTTCCCTCGCGGAGAAGTCACCAAAGTA
GGCTACCGCATAAAAGAGGAAACGCTTGAGCGTGTATGGTGGCGTTACCCCGATACACCTGCCGGCCAACAAGGTGTGAT
TACTCCTCTGCTTGATGATGTTGAGAACCTGGAGTTCGAGTTTTATGACGGAAGCCGCTGGGGCAAAGAGTGGCAAACCG
ATAAATCACTGCCTAAAGCGGTGAGACTTAAGCTGACACTGAAAGATTACGGTGAGATAGAACGTGTTTATCTCACACCA
AGTGGCGCCTTAGATCAGGTCGATGATTCTTCAAACAGTGACTCTTCAGGCAGTAATGAGGGGAATAATGATTCATCAAA
CTAA

Upstream 100 bases:

>100_bases
AAGGTGACTCCCATCGATACCAGCGATAATTTATTAAAGGCGTTTGATGTGAGTGCGGCAACAAGTAAGAAAGCTTCTCC
AGTGGTCACGGTGCGCAGCT

Downstream 100 bases:

>100_bases
TAAGCCTGCACTGACAAGGTCTGCTGTGGGGCGTAAACAGCGTGGTGTCGCGCTGATCATTATTCTGATGCTTTTGGCGA
TCATGGCAACCATAGCTGGC

Product: type II secretory pathway subunit EpsJ

Products: NA

Alternate protein names: Cholera toxin secretion protein epsJ [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MWSIKSVWSTKSMWLSKIVSLAKSKSLSKSMSANKHTPRKQGISSKGRGFTLIEVLVSIAIFSTLSMAAYQVVNQVQRSN
ELSIERSARLNQLQRSLVILDNDFRQMAVRQFRTNGEEASSKLILMKEYLLDSDSVGIMFTRLGWHNPQQQFPRGEVTKV
GYRIKEETLERVWWRYPDTPAGQQGVITPLLDDVENLEFEFYDGSRWGKEWQTDKSLPKAVRLKLTLKDYGEIERVYLTP
SGALDQVDDSSNSDSSGSNEGNNDSSN

Sequences:

>Translated_267_residues
MWSIKSVWSTKSMWLSKIVSLAKSKSLSKSMSANKHTPRKQGISSKGRGFTLIEVLVSIAIFSTLSMAAYQVVNQVQRSN
ELSIERSARLNQLQRSLVILDNDFRQMAVRQFRTNGEEASSKLILMKEYLLDSDSVGIMFTRLGWHNPQQQFPRGEVTKV
GYRIKEETLERVWWRYPDTPAGQQGVITPLLDDVENLEFEFYDGSRWGKEWQTDKSLPKAVRLKLTLKDYGEIERVYLTP
SGALDQVDDSSNSDSSGSNEGNNDSSN
>Mature_267_residues
MWSIKSVWSTKSMWLSKIVSLAKSKSLSKSMSANKHTPRKQGISSKGRGFTLIEVLVSIAIFSTLSMAAYQVVNQVQRSN
ELSIERSARLNQLQRSLVILDNDFRQMAVRQFRTNGEEASSKLILMKEYLLDSDSVGIMFTRLGWHNPQQQFPRGEVTKV
GYRIKEETLERVWWRYPDTPAGQQGVITPLLDDVENLEFEFYDGSRWGKEWQTDKSLPKAVRLKLTLKDYGEIERVYLTP
SGALDQVDDSSNSDSSGSNEGNNDSSN

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

Organism=Escherichia coli, GI1789728, Length=197, Percent_Identity=26.3959390862944, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 30341; Mature: 30341

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSIKSVWSTKSMWLSKIVSLAKSKSLSKSMSANKHTPRKQGISSKGRGFTLIEVLVSIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHH
IFSTLSMAAYQVVNQVQRSNELSIERSARLNQLQRSLVILDNDFRQMAVRQFRTNGEEAS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCHHHH
SKLILMKEYLLDSDSVGIMFTRLGWHNPQQQFPRGEVTKVGYRIKEETLERVWWRYPDTP
HHEEHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
AGQQGVITPLLDDVENLEFEFYDGSRWGKEWQTDKSLPKAVRLKLTLKDYGEIERVYLTP
CCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCHHEEEEEEECCCCCEEEEEECC
SGALDQVDDSSNSDSSGSNEGNNDSSN
CCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MWSIKSVWSTKSMWLSKIVSLAKSKSLSKSMSANKHTPRKQGISSKGRGFTLIEVLVSIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHH
IFSTLSMAAYQVVNQVQRSNELSIERSARLNQLQRSLVILDNDFRQMAVRQFRTNGEEAS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCHHHH
SKLILMKEYLLDSDSVGIMFTRLGWHNPQQQFPRGEVTKVGYRIKEETLERVWWRYPDTP
HHEEHHHHHHCCCCCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
AGQQGVITPLLDDVENLEFEFYDGSRWGKEWQTDKSLPKAVRLKLTLKDYGEIERVYLTP
CCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCHHEEEEEEECCCCCEEEEEECC
SGALDQVDDSSNSDSSGSNEGNNDSSN
CCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]