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The map label for this gene is epsI [H]

Identifier: 218708204

GI number: 218708204

Start: 135235

End: 135630

Strand: Direct

Name: epsI [H]

Synonym: VS_0140

Alternate gene names: 218708204

Gene position: 135235-135630 (Clockwise)

Preceding gene: 218708203

Following gene: 218708205

Centisome position: 4.1

GC content: 47.22

Gene sequence:

>396_bases
ATGAAGAGGAATAACCGTTCTCCTTATCGTTCTCGTGGTATGCCTCTTGGTTCTCGAGGAATGACTCTGCTTGAAGTATT
AGTTGCGCTCGCTATCTTCGCTACGGCGGCGATCAGTGTGATTCGTGCTGTCACCCAACACATTAATACCCTTAGTTATC
TAGAAGAAAAAACCTTCGCGGCGATGGTTGTTGATAACCAGATGGCCTTAGTCATGCTGCATCCTGAGAAGCTTAAAAAA
GCGCAGGGCACGCAAGAGTTAGCGGGAAGAGAGTGGTTCTGGAAGGTGACTCCCATCGATACCAGCGATAATTTATTAAA
GGCGTTTGATGTGAGTGCGGCAACAAGTAAGAAAGCTTCTCCAGTGGTCACGGTGCGCAGCTATGTGGTCAATTAA

Upstream 100 bases:

>100_bases
TTGTCGTTTTATCCAAATACCGGAGACACCATACAAGATGGTTGGCGTATTCGAGTGTTGGATAATGGTGTGATTCGACT
GCTAGAGCCGGGAGAAGAAG

Downstream 100 bases:

>100_bases
AAGCGTGTGGTCAACTAAAAGCATGTGGTTAAGTAAAATCGTGTCGTTAGCGAAGAGCAAGTCGCTAAGTAAAAGCATGT
CGGCAAATAAGCATACGCCG

Product: type II secretory pathway, pseudopilin EpsI

Products: NA

Alternate protein names: Cholera toxin secretion protein epsI [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MKRNNRSPYRSRGMPLGSRGMTLLEVLVALAIFATAAISVIRAVTQHINTLSYLEEKTFAAMVVDNQMALVMLHPEKLKK
AQGTQELAGREWFWKVTPIDTSDNLLKAFDVSAATSKKASPVVTVRSYVVN

Sequences:

>Translated_131_residues
MKRNNRSPYRSRGMPLGSRGMTLLEVLVALAIFATAAISVIRAVTQHINTLSYLEEKTFAAMVVDNQMALVMLHPEKLKK
AQGTQELAGREWFWKVTPIDTSDNLLKAFDVSAATSKKASPVVTVRSYVVN
>Mature_131_residues
MKRNNRSPYRSRGMPLGSRGMTLLEVLVALAIFATAAISVIRAVTQHINTLSYLEEKTFAAMVVDNQMALVMLHPEKLKK
AQGTQELAGREWFWKVTPIDTSDNLLKAFDVSAATSKKASPVVTVRSYVVN

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP I family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010052
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR003413 [H]

Pfam domain/function: PF02501 GSPII_IJ; PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 14509; Mature: 14509

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNNRSPYRSRGMPLGSRGMTLLEVLVALAIFATAAISVIRAVTQHINTLSYLEEKTFA
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMVVDNQMALVMLHPEKLKKAQGTQELAGREWFWKVTPIDTSDNLLKAFDVSAATSKKAS
EEEECCCEEEEEECHHHHHHHCCHHHHCCCCEEEEECCCCCCCCHHHHHCCCHHCCCCCC
PVVTVRSYVVN
CHHHHHHHHCC
>Mature Secondary Structure
MKRNNRSPYRSRGMPLGSRGMTLLEVLVALAIFATAAISVIRAVTQHINTLSYLEEKTFA
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMVVDNQMALVMLHPEKLKKAQGTQELAGREWFWKVTPIDTSDNLLKAFDVSAATSKKAS
EEEECCCEEEEEECHHHHHHHCCHHHHCCCCEEEEECCCCCCCCHHHHHCCCHHCCCCCC
PVVTVRSYVVN
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]