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The map label for this gene is epsK [H]

Identifier: 218708206

GI number: 218708206

Start: 136404

End: 137453

Strand: Direct

Name: epsK [H]

Synonym: VS_0142

Alternate gene names: 218708206

Gene position: 136404-137453 (Clockwise)

Preceding gene: 218708205

Following gene: 218708207

Centisome position: 4.13

GC content: 46.29

Gene sequence:

>1050_bases
ATGATTCATCAAACTAATAAGCCTGCACTGACAAGGTCTGCTGTGGGGCGTAAACAGCGTGGTGTCGCGCTGATCATTAT
TCTGATGCTTTTGGCGATCATGGCAACCATAGCTGGCAGTATGTCGGAGCGTCTGTTTACGCAATTCAAGCGCGTTGGCA
ATCAACTGAATTACCAACAGGCTTACTGGTATAGCATTGGTGTAGAAGCGCTTGTCCAAGACGGTATTAGGCAAAGTTAT
AAAGATAGTGATACGGTGAACTTAAGCCAGCCATGGGCGTTAGAAGAGCAGGTATACCCATTGGATTATGGCCAAGTTAA
AGGGCGTATCGTTGATGCGCAGGCTTGTTTCAATCTTAATGCGCTCGCTGGGATTACGACGACATCAAGTAACCAAACCC
CTTATTTAGTGAAGGTTTGGCAAACCTTACTGGAAAACCAAGATGTGGAGCCTTATCAGGCTGAGGTTATCGCAAATTCA
AGTTGGGAATTTCTGGACTCTGATACTCGAGCCACCTCTTCTACGGGTGTAGAAGACAGCACTTATGAGGCGATGAAGCC
AGCTTATTTGGCAGCGAATGGCTTAATAGCGGATGAATCGGAGCTGCGAGCCGTTTATCAGGTCTCTGGTGAGGCAATGG
AAAAGGTACATCCATTTATTTGTGCTTTGCCCACTGATGATTTCCGATTAAACGTGAATACCATCTCGGAAAAGCAGGCA
TCTTTACTGGAAGCAATGTTTGCACCCGGTCTAAGTGAATCGGACGCCAAACAGCTGATAGACAAGCGCCCATTTGATGG
CTGGGATTCGGTGGATGCGTTTATGGCTGAGCCTGCTATTGTTGGTGTGAGTGCTGAAATCAGCAAACAAGCGAAAGCAT
ATTTAACTGTAGATAGCGCCTATTTTGAGCTAGATGCAGAGGTATTAGTTGAGCAGTCTCGTGTACGTATACGAACGCTT
TTCTATAGTAGTAATAGAGAAACAGTGACGGTAGTACGCCGTCGTTTTGGAGGAATCCGTGAGCGAGTTTCTGACCGTTC
GACTGAGTAG

Upstream 100 bases:

>100_bases
TACGGTGAGATAGAACGTGTTTATCTCACACCAAGTGGCGCCTTAGATCAGGTCGATGATTCTTCAAACAGTGACTCTTC
AGGCAGTAATGAGGGGAATA

Downstream 100 bases:

>100_bases
CGAGCCACAAAGCCCAGTGCAGTGGTTGGTTTGGTCGACAAGCCAACAAGAAGTGATAGCAAGTGGTGAGCTGTCTAGCT
GGGATCAGCTTGATGAGTTA

Product: type II secretory pathway subunit EpsK

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIHQTNKPALTRSAVGRKQRGVALIIILMLLAIMATIAGSMSERLFTQFKRVGNQLNYQQAYWYSIGVEALVQDGIRQSY
KDSDTVNLSQPWALEEQVYPLDYGQVKGRIVDAQACFNLNALAGITTTSSNQTPYLVKVWQTLLENQDVEPYQAEVIANS
SWEFLDSDTRATSSTGVEDSTYEAMKPAYLAANGLIADESELRAVYQVSGEAMEKVHPFICALPTDDFRLNVNTISEKQA
SLLEAMFAPGLSESDAKQLIDKRPFDGWDSVDAFMAEPAIVGVSAEISKQAKAYLTVDSAYFELDAEVLVEQSRVRIRTL
FYSSNRETVTVVRRRFGGIRERVSDRSTE

Sequences:

>Translated_349_residues
MIHQTNKPALTRSAVGRKQRGVALIIILMLLAIMATIAGSMSERLFTQFKRVGNQLNYQQAYWYSIGVEALVQDGIRQSY
KDSDTVNLSQPWALEEQVYPLDYGQVKGRIVDAQACFNLNALAGITTTSSNQTPYLVKVWQTLLENQDVEPYQAEVIANS
SWEFLDSDTRATSSTGVEDSTYEAMKPAYLAANGLIADESELRAVYQVSGEAMEKVHPFICALPTDDFRLNVNTISEKQA
SLLEAMFAPGLSESDAKQLIDKRPFDGWDSVDAFMAEPAIVGVSAEISKQAKAYLTVDSAYFELDAEVLVEQSRVRIRTL
FYSSNRETVTVVRRRFGGIRERVSDRSTE
>Mature_349_residues
MIHQTNKPALTRSAVGRKQRGVALIIILMLLAIMATIAGSMSERLFTQFKRVGNQLNYQQAYWYSIGVEALVQDGIRQSY
KDSDTVNLSQPWALEEQVYPLDYGQVKGRIVDAQACFNLNALAGITTTSSNQTPYLVKVWQTLLENQDVEPYQAEVIANS
SWEFLDSDTRATSSTGVEDSTYEAMKPAYLAANGLIADESELRAVYQVSGEAMEKVHPFICALPTDDFRLNVNTISEKQA
SLLEAMFAPGLSESDAKQLIDKRPFDGWDSVDAFMAEPAIVGVSAEISKQAKAYLTVDSAYFELDAEVLVEQSRVRIRTL
FYSSNRETVTVVRRRFGGIRERVSDRSTE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=325, Percent_Identity=32.6153846153846, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 38932; Mature: 38932

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHQTNKPALTRSAVGRKQRGVALIIILMLLAIMATIAGSMSERLFTQFKRVGNQLNYQQ
CCCCCCCCCHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AYWYSIGVEALVQDGIRQSYKDSDTVNLSQPWALEEQVYPLDYGQVKGRIVDAQACFNLN
HHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCH
ALAGITTTSSNQTPYLVKVWQTLLENQDVEPYQAEVIANSSWEFLDSDTRATSSTGVEDS
HHEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHEEEECCCCHHHCCCCCCCCCCCCCCC
TYEAMKPAYLAANGLIADESELRAVYQVSGEAMEKVHPFICALPTDDFRLNVNTISEKQA
HHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECHHHHHHH
SLLEAMFAPGLSESDAKQLIDKRPFDGWDSVDAFMAEPAIVGVSAEISKQAKAYLTVDSA
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCHHHHHCCEEEEEEECH
YFELDAEVLVEQSRVRIRTLFYSSNRETVTVVRRRFGGIRERVSDRSTE
HHHCCHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIHQTNKPALTRSAVGRKQRGVALIIILMLLAIMATIAGSMSERLFTQFKRVGNQLNYQQ
CCCCCCCCCHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AYWYSIGVEALVQDGIRQSYKDSDTVNLSQPWALEEQVYPLDYGQVKGRIVDAQACFNLN
HHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCH
ALAGITTTSSNQTPYLVKVWQTLLENQDVEPYQAEVIANSSWEFLDSDTRATSSTGVEDS
HHEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHEEEECCCCHHHCCCCCCCCCCCCCCC
TYEAMKPAYLAANGLIADESELRAVYQVSGEAMEKVHPFICALPTDDFRLNVNTISEKQA
HHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECHHHHHHH
SLLEAMFAPGLSESDAKQLIDKRPFDGWDSVDAFMAEPAIVGVSAEISKQAKAYLTVDSA
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCHHHHHCCEEEEEEECH
YFELDAEVLVEQSRVRIRTLFYSSNRETVTVVRRRFGGIRERVSDRSTE
HHHCCHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]