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The map label for this gene is papA

Identifier: 218702822

GI number: 218702822

Start: 4785833

End: 4786429

Strand: Direct

Name: papA

Synonym: ECIAI39_4587

Alternate gene names: 218702822

Gene position: 4785833-4786429 (Clockwise)

Preceding gene: 218702821

Following gene: 218702823

Centisome position: 93.25

GC content: 44.39

Gene sequence:

>597_bases
GTGTGTTCAGTAATGAAAAAGAGGTTGTTATTTATGATTAAGTCGGTTATTGCCGGTGCGGTAGCTATGGCAGTGGTGTC
TTTTGGTGCATATGCTGCTCCAACTATTCCTCAGGGGCAGGGTAAAGTAACTTTTAACGGAACTGTTGTTACTGCTCCAT
GCGGCATTTCTCAGAAATCAGCTGATCAGTCTATTGATTTTGGGCAGCTTTCAAAAAGCTTCCTTGCGGCAGGAGGTGTA
TCCAAACCAATGAATTTAGATATTGAATTGGTTAATTGTGATATCACTTCATTTAAGGGGGGGGGAGGAAGCCAGGCAGC
AAAAAAAGGGACTGTGAAGCTGGCTTTTAGTGGTCCAAGGGTTTCTGGTCATAATGAGGAGTTAGATACCAGCGGGGGGA
CAGGTACTGCAATTGTAGTTCAGGCTGCAGGTAAAAACGTTTCTTTCGATGGCACAGAAGGTGATGCTAATACCCTGAAA
GATGGAGATAATGTGCTGCATTATACTGCTGTTGTTAAGAAGTCGTCAGCCGCTGGTGCCACTGTTACTGAAGGTGCCTT
CTCAGCAGTTGCGAATTTCAACCTGACTTATCAGTAA

Upstream 100 bases:

>100_bases
TTCTGTCGATAAATAACCTGCCCTGAAATACGAGAATATTATTTGTATTGATCTGGTTATTAAAGGTAATCGGGTCATTT
TAAATTGCCAGATATCTCTG

Downstream 100 bases:

>100_bases
TACTGATAATCCGGTCTGATAAACAGCGGATTTTTCCGCTGTTTATTTCTCAGGGTATTTATCATGAGACTGCGATTCTC
TGTTCCACTTTTCTTTTTTG

Product: major pilin protein PapA

Products: NA

Alternate protein names: Pap pili [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MCSVMKKRLLFMIKSVIAGAVAMAVVSFGAYAAPTIPQGQGKVTFNGTVVTAPCGISQKSADQSIDFGQLSKSFLAAGGV
SKPMNLDIELVNCDITSFKGGGGSQAAKKGTVKLAFSGPRVSGHNEELDTSGGTGTAIVVQAAGKNVSFDGTEGDANTLK
DGDNVLHYTAVVKKSSAAGATVTEGAFSAVANFNLTYQ

Sequences:

>Translated_198_residues
MCSVMKKRLLFMIKSVIAGAVAMAVVSFGAYAAPTIPQGQGKVTFNGTVVTAPCGISQKSADQSIDFGQLSKSFLAAGGV
SKPMNLDIELVNCDITSFKGGGGSQAAKKGTVKLAFSGPRVSGHNEELDTSGGTGTAIVVQAAGKNVSFDGTEGDANTLK
DGDNVLHYTAVVKKSSAAGATVTEGAFSAVANFNLTYQ
>Mature_198_residues
MCSVMKKRLLFMIKSVIAGAVAMAVVSFGAYAAPTIPQGQGKVTFNGTVVTAPCGISQKSADQSIDFGQLSKSFLAAGGV
SKPMNLDIELVNCDITSFKGGGGSQAAKKGTVKLAFSGPRVSGHNEELDTSGGTGTAIVVQAAGKNVSFDGTEGDANTLK
DGDNVLHYTAVVKKSSAAGATVTEGAFSAVANFNLTYQ

Specific function: Polymerizes to form the thick (6.8 nm in diameter) rod of the pilus (also called fimbria). The rod is a right-handed helical cylinder with 3.28 papA subunits per turn. Pili are polar filaments radiating from the surface of the bacterium to a length of 0.5

COG id: COG3539

COG function: function code NU; P pilus assembly protein, pilin FimA

Gene ontology:

Cell location: Secreted. Fimbrium [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial protein family [H]

Homologues:

Organism=Escherichia coli, GI1786938, Length=138, Percent_Identity=31.1594202898551, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008966
- InterPro:   IPR000259
- InterPro:   IPR014779 [H]

Pfam domain/function: PF00419 Fimbrial [H]

EC number: NA

Molecular weight: Translated: 20093; Mature: 20093

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSVMKKRLLFMIKSVIAGAVAMAVVSFGAYAAPTIPQGQGKVTFNGTVVTAPCGISQKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEECCCCCCCCC
ADQSIDFGQLSKSFLAAGGVSKPMNLDIELVNCDITSFKGGGGSQAAKKGTVKLAFSGPR
CCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCC
VSGHNEELDTSGGTGTAIVVQAAGKNVSFDGTEGDANTLKDGDNVLHYTAVVKKSSAAGA
CCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
TVTEGAFSAVANFNLTYQ
EECHHHHHHHHEEEEEEC
>Mature Secondary Structure
MCSVMKKRLLFMIKSVIAGAVAMAVVSFGAYAAPTIPQGQGKVTFNGTVVTAPCGISQKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEECCCCCCCCC
ADQSIDFGQLSKSFLAAGGVSKPMNLDIELVNCDITSFKGGGGSQAAKKGTVKLAFSGPR
CCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCC
VSGHNEELDTSGGTGTAIVVQAAGKNVSFDGTEGDANTLKDGDNVLHYTAVVKKSSAAGA
CCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
TVTEGAFSAVANFNLTYQ
EECHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6140260; 1357526; 10049807 [H]