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The map label for this gene is scrB [H]

Identifier: 218701266

GI number: 218701266

Start: 3070872

End: 3072281

Strand: Direct

Name: scrB [H]

Synonym: ECIAI39_2966

Alternate gene names: 218701266

Gene position: 3070872-3072281 (Clockwise)

Preceding gene: 218701265

Following gene: 218701267

Centisome position: 59.84

GC content: 48.65

Gene sequence:

>1410_bases
ATGTCGATGCCTTCACAAATGCCCGCGATTTTACAGGCAGTGATGAAAGGTCAGTCCAAAGCACTGGCAGATGCCCACTA
TCCTTGTTGGCATCTCGCTCCCGTCACCGGACTAATGAACGATCCAAATGGCTTTTGCTGGTCTGGAGGCCGCTATCATC
TGTTTTATCAGTGGAATCCACTGGCTTGTGATCATAAGTACAAATGTTGGGGGCACTGGAGTTCGACGGATTTATTGCAC
TGGCAGCATGAACCATTAGCGCTAATGCCCGATAAAGAGTACGACCGTAATGGTTGTTACTCAGGAAGCGCGGTTAATAA
TCAGGGCGTTCTCACACTTTGCTATACCGGTAATGTTAAGTTTGATGATGGCAGCAGAACGGCATGGCAATGCCTGGCCA
CCGAAAATAACCAGGGCGGGTTTGATAAACTAGGCCCTGTCATACCGTTACCTGATGGTTACACCGGACATGTTCGTGAT
CCAAAAGTCTGGAAGCACAACAGCCAATGGTACATGGTGCTTGGCGCGCAAGATAAAGAGAAACGGGGGAAAGTGCTGCT
GTATTCCTCTGTCGATCTCTATACCTGGTCTTTTCATGGAGAAATTGCTGGTAACGGTCTGAATGAAATAGATAACGCAG
GATACATGTGGGAATGTCCTGATTTTTTCGCCCTTGATGGTGAATACATTCTCCTCTGTTGCCCCCAGGGAATGGTGCGC
GAACATGAGCGCTATCTGAATACTTATCCCTGCGCCTGGCTACATGGACAGTTTGATTACGAGACAGGCAAATTTACGCA
TGGCGCTTTCTCAGAGCTGGATGCGGGATTTGAATTCTATGCACCACAGACGATGGAAGCCCCGGACGGGCGACGATTGC
TGGTCGGCTGGATGGGGGTTCCTGATGGCGAAGAGATGTTGCAACCAACCAGAAAGCATCATTGGCAGCATCAAATGACC
TGTTTTCGCGAACTCTCATTCCAGAAGGGAAAATTGTTTCAGATGCCAATCAGGGAACTCGCGCAACTGCGTGAAGCTGA
ACACTTCTGGCAAGGAAAAGCAGATCATGCGCCGCATGTCGAAATTGAACGCCTGGAAATGGACATCATTCCATCAGGCG
AGCTACATCTGAATTTTGGCAACGCACTGGCACTGCATCTTAATGACGATGGTATTCAACTACAGAGGAAAAGCCTTGCA
GGGCAAGAGAAACTAACTCGTTACTGGCGAGGAAGCGTAACGTCACTGAAGATTCTCTGTGACAGTTCCAGCATAGAGAT
ATTCATCAATAATGGCGAAGGCGTAATGAGCAATCGTTATTTTCCCCATCATCCAGCTTCGCTGATATTACAGGGCGAGT
CTGACGTCACGTTACACTACTGGTCGCTACGCGCCTGCATGGTAGAATGA

Upstream 100 bases:

>100_bases
AGCCAACATCGTTGGTTAATTATATTATTGGAATGGTGATTGCTTTCGCTGTCGCTTTTAGCCTCTCTTTATTACTCAAA
TACAAAACGGACGAGGAGTA

Downstream 100 bases:

>100_bases
GGGTTTTGAATTACGGATCCTCGTCGTGAGAAAAACCAAACGCGTTACTATTAAAGACATTGCTGAACTGGCGGGAGTGT
CGAAAGCAACCGCCAGTCTG

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNGFCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLH
WQHEPLALMPDKEYDRNGCYSGSAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWECPDFFALDGEYILLCCPQGMVR
EHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAGFEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMT
CFRELSFQKGKLFQMPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELHLNFGNALALHLNDDGIQLQRKSLA
GQEKLTRYWRGSVTSLKILCDSSSIEIFINNGEGVMSNRYFPHHPASLILQGESDVTLHYWSLRACMVE

Sequences:

>Translated_469_residues
MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNGFCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLH
WQHEPLALMPDKEYDRNGCYSGSAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWECPDFFALDGEYILLCCPQGMVR
EHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAGFEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMT
CFRELSFQKGKLFQMPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELHLNFGNALALHLNDDGIQLQRKSLA
GQEKLTRYWRGSVTSLKILCDSSSIEIFINNGEGVMSNRYFPHHPASLILQGESDVTLHYWSLRACMVE
>Mature_468_residues
SMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNGFCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHW
QHEPLALMPDKEYDRNGCYSGSAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRDP
KVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWECPDFFALDGEYILLCCPQGMVRE
HERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAGFEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTC
FRELSFQKGKLFQMPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELHLNFGNALALHLNDDGIQLQRKSLAG
QEKLTRYWRGSVTSLKILCDSSSIEIFINNGEGVMSNRYFPHHPASLILQGESDVTLHYWSLRACMVE

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=347, Percent_Identity=26.2247838616715, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 53618; Mature: 53487

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNGFCWSGGRYHLFYQWNP
CCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEECCHHCCCCCCCCCEECCCEEEEEEEECC
LACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSGSAVNNQGVLTLCYTGNVK
CCCCCCEEEEECCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEE
FDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRDPKVWKHNSQWYMVLGAQDKE
ECCCCCCEEEEEEECCCCCCHHHCCCEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCC
KRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWECPDFFALDGEYILLCCPQGMVR
CCCCEEEEEECEEEEEEEECEECCCCCCHHCCCCEEEECCCEEEECCCEEEEECCCHHHH
EHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAGFEFYAPQTMEAPDGRRLLVGWMGV
HHHHHHCCCCEEEEECEEECCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCEEEEEEECC
PDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQMPIRELAQLREAEHFWQGKADHAPHV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
EIERLEMDIIPSGELHLNFGNALALHLNDDGIQLQRKSLAGQEKLTRYWRGSVTSLKILC
EEEEEEEEECCCCEEEEEECCEEEEEECCCCCEEEHHHHCCHHHHHHHHCCCCEEEEEEE
DSSSIEIFINNGEGVMSNRYFPHHPASLILQGESDVTLHYWSLRACMVE
CCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCEEEEEEEEEEEECC
>Mature Secondary Structure 
SMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNGFCWSGGRYHLFYQWNP
CCCHHHHHHHHHHHCCCCHHHHCCCCCEEEECCHHCCCCCCCCCEECCCEEEEEEEECC
LACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSGSAVNNQGVLTLCYTGNVK
CCCCCCEEEEECCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEE
FDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRDPKVWKHNSQWYMVLGAQDKE
ECCCCCCEEEEEEECCCCCCHHHCCCEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCC
KRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWECPDFFALDGEYILLCCPQGMVR
CCCCEEEEEECEEEEEEEECEECCCCCCHHCCCCEEEECCCEEEECCCEEEEECCCHHHH
EHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAGFEFYAPQTMEAPDGRRLLVGWMGV
HHHHHHCCCCEEEEECEEECCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCEEEEEEECC
PDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQMPIRELAQLREAEHFWQGKADHAPHV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
EIERLEMDIIPSGELHLNFGNALALHLNDDGIQLQRKSLAGQEKLTRYWRGSVTSLKILC
EEEEEEEEECCCCEEEEEECCEEEEEECCCCCEEEHHHHCCHHHHHHHHCCCCEEEEEEE
DSSSIEIFINNGEGVMSNRYFPHHPASLILQGESDVTLHYWSLRACMVE
CCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412665; 8628219 [H]