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The map label for this gene is scrA [H]

Identifier: 218701265

GI number: 218701265

Start: 3069502

End: 3070872

Strand: Direct

Name: scrA [H]

Synonym: ECIAI39_2965

Alternate gene names: 218701265

Gene position: 3069502-3070872 (Clockwise)

Preceding gene: 218701264

Following gene: 218701266

Centisome position: 59.81

GC content: 46.1

Gene sequence:

>1371_bases
ATGGATTTCGATAAAATCGCCCAATCGTTGCTTCCTTTATTAGGTGGTAAGGAGAACATCACCAGTGCAGCACATTGCGC
CACTCGCCTACGGCTGGTACTGGTTGACGACACACTTGCCGATCAACATGCCATTGGCCAGATTGATGGAGTGAAAGGTT
GTTTTCGTAATTCAGGGCAAATGCAAATCATCTTTGGCACCGGCGTGGTTAATAAAGTCTATGCTGCCTTTATTCAGGTT
GCAGGTATTAGCGAATCCAGTAAAGCAGATACCGCTCGACTCGCCGCTCAAAAACTCAATCCTTTTCAGCGAATAGCACG
GCTACTTTCTAATATTTTTGTCCCCATCATTCCAGCCATTGTTGCTTCTGGTTTATTAATGGGGCTTCTGGGAATGGTGA
AAACATATGGCTGGGTGAATGCCGATAATGCGATTTATATCCTGCTGGATATGTGCAGTTCAGCCGCATTTATTATTCTC
CCCATCCTTATTGGCTTTACTGCTGCTCGTGAATTTGGTGGAAATCCTTATCTTGGCGCGACATTAGGAGGGATTCTGAC
TCACCCGGCACTCACAAATGCCTGGGGCGTTGCTGCGGGATTTCAGACAATGAACTTCTTCGGTTTTGAAATCGCCATGA
TTGGCTATCAGGGAACGGTTTTCCCCGTGCTTCTGGCAGTATGGTTTATGAGCATAGTGGAAAAACAGTTACGCAGGTTT
ATCCCTGATGCTCTGGATCTCATTCTGACGCCATTTCTGACTGTCGTCATTTCTGGCTTTATCGCTCTTTTGATTATTGG
CCCGGCAGGACGAGCGTTAGGTGACGGTATCTCTTTCGTTCTCAGCACGCTGATTGCACACGCAGGCTGGTTAGCAGGGT
TGTTATTCGGTGGACTATATTCAGCGATTGTTATTACGGGTATTCATCATAGCTTCCACGCAATTGAAGCAGGACTTTTA
GGTAACCCTGCAATCGGTGTTAATTTCCTGCTGCCTATTTGGGCAATGGCAAACGTTGCGCAAGGCGGTGCATGTCTGGC
GGTATGGTTTAAAACCAAAGATACAAAAATTAAAGCAATCACCCTACCCTCGGCCTTTTCCGCAATGTTAGGGATTACTG
AAGCCGCTATTTTTGGTATCAACCTTCGTTTTGTTAAACCTTTTATTGCGGCCTTAATTGGTGGCGCTGCCGGCGGTGCC
TGGGTTGTCTCTGTTCACGTTTATATGACTGCCGTTGGTCTGACCGCAATACCCGGCATGGCAATAGTTCAGCCAACATC
GTTGGTTAATTATATTATTGGAATGGTGATTGCTTTCGCTGTCGCTTTTAGCCTCTCTTTATTACTCAAATACAAAACGG
ACGAGGAGTAA

Upstream 100 bases:

>100_bases
AGAGTGGAATTTTGGTGTCCAAATGGAAACCTGGTTTTAATACCAGACAGCCTCACGGATGTGAGGCTTTTACTGAAATA
ACAATGAATTAGAGGGTTAT

Downstream 100 bases:

>100_bases
TGTCGATGCCTTCACAAATGCCCGCGATTTTACAGGCAGTGATGAAAGGTCAGTCCAAAGCACTGGCAGATGCCCACTAT
CCTTGTTGGCATCTCGCTCC

Product: PTS system sucrose-specific EIIBC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDFDKIAQSLLPLLGGKENITSAAHCATRLRLVLVDDTLADQHAIGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQV
AGISESSKADTARLAAQKLNPFQRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQGTVFPVLLAVWFMSIVEKQLRRF
IPDALDLILTPFLTVVISGFIALLIIGPAGRALGDGISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLL
GNPAIGVNFLLPIWAMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALIGGAAGGA
WVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLKYKTDEE

Sequences:

>Translated_456_residues
MDFDKIAQSLLPLLGGKENITSAAHCATRLRLVLVDDTLADQHAIGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQV
AGISESSKADTARLAAQKLNPFQRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQGTVFPVLLAVWFMSIVEKQLRRF
IPDALDLILTPFLTVVISGFIALLIIGPAGRALGDGISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLL
GNPAIGVNFLLPIWAMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALIGGAAGGA
WVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLKYKTDEE
>Mature_456_residues
MDFDKIAQSLLPLLGGKENITSAAHCATRLRLVLVDDTLADQHAIGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQV
AGISESSKADTARLAAQKLNPFQRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQGTVFPVLLAVWFMSIVEKQLRRF
IPDALDLILTPFLTVVISGFIALLIIGPAGRALGDGISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLL
GNPAIGVNFLLPIWAMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALIGGAAGGA
WVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLKYKTDEE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=436, Percent_Identity=33.256880733945, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1790159, Length=468, Percent_Identity=30.3418803418803, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI48994906, Length=467, Percent_Identity=29.9785867237687, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788769, Length=478, Percent_Identity=31.1715481171548, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 48173; Mature: 48173

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDKIAQSLLPLLGGKENITSAAHCATRLRLVLVDDTLADQHAIGQIDGVKGCFRNSGQ
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEECCCCCHHHCCCCCCHHHHHCCCCC
MQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPFQRIARLLSNIFVPIIPAI
EEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIILPILIGFTAAREFGGNPYLGA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHH
TLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQGTVFPVLLAVWFMSIVEKQLRRF
HHHHHHHCCCHHCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IPDALDLILTPFLTVVISGFIALLIIGPAGRALGDGISFVLSTLIAHAGWLAGLLFGGLY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIWAMANVAQGGACLAVWFKTKDTKIKAI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEE
TLPSAFSAMLGITEAAIFGINLRFVKPFIAALIGGAAGGAWVVSVHVYMTAVGLTAIPGM
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
AIVQPTSLVNYIIGMVIAFAVAFSLSLLLKYKTDEE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDFDKIAQSLLPLLGGKENITSAAHCATRLRLVLVDDTLADQHAIGQIDGVKGCFRNSGQ
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEECCCCCHHHCCCCCCHHHHHCCCCC
MQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPFQRIARLLSNIFVPIIPAI
EEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIILPILIGFTAAREFGGNPYLGA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHH
TLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQGTVFPVLLAVWFMSIVEKQLRRF
HHHHHHHCCCHHCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IPDALDLILTPFLTVVISGFIALLIIGPAGRALGDGISFVLSTLIAHAGWLAGLLFGGLY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIWAMANVAQGGACLAVWFKTKDTKIKAI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEE
TLPSAFSAMLGITEAAIFGINLRFVKPFIAALIGGAAGGAWVVSVHVYMTAVGLTAIPGM
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
AIVQPTSLVNYIIGMVIAFAVAFSLSLLLKYKTDEE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8628219 [H]