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The map label for this gene is scrR [H]

Identifier: 218701267

GI number: 218701267

Start: 3072307

End: 3073314

Strand: Direct

Name: scrR [H]

Synonym: ECIAI39_2967

Alternate gene names: 218701267

Gene position: 3072307-3073314 (Clockwise)

Preceding gene: 218701266

Following gene: 218701269

Centisome position: 59.86

GC content: 47.12

Gene sequence:

>1008_bases
GTGAGAAAAACCAAACGCGTTACTATTAAAGACATTGCTGAACTGGCGGGAGTGTCGAAAGCAACCGCCAGTCTGGTTCT
CAATGGTCGTGGCAAAGAGTTACGTGTTGCGCAGGAAACGCGTGAGCGTGTGCTGAGTATTGCCCAACAGCAGCATTATC
AGCCCAGCATTCATGCCCGCTCATTACGGGACAACCGCAGTCATACCATTGGTCTTGTCGTTCCTGAAATCACTAACTAC
GGATTTGCAGTATTTTCCCATGAACTGGAAACATTGTGTCGGGAAGCGGGTGTCCAGCTCCTCATATCCTGTACTGATGA
AAATCCTTCACAGGAAACAATGGTGGTGAACAATATGATTGCCCGCCAGGTTGATGGGTTAATCGTCGCATCCAGTATGC
TGCATGATAATGACTATCAAAAACTTAGCGAGCAATTACCTGTTGTGTTGTTCGATCGCCACATGAACGGCAGCACATTG
CCATTGGTCATTACTGACTCAGTTTCTCCCACCGCCACCCTGGTGGCAGATATTGCACGAAGTCACCCGGATGAATTCTA
TTTTCTGGGGGGACAACCCCGTTTGTCACCGACTCGCGATCGACTGGAAGGATTTACACAAGGCTTGCAGCAAGCCGGAG
TAACGCTACAACCTGAATGGATTATTCACGGTAATTATCATCCGAGCTCAGGCTATGAAATGTTTGCCGCGCTATGCGCT
CGTTTAGGACGTCCCCCTAAAGCCTTATTCACTGCCGCTTGTGGATTACTGGAAGGCGTTCTGCGCTATATGAGCCAATA
TAATTTACTCGACAGTAAAATCCACCTGGCGAGTTTTGATGATCATTACTTGTATGATTCACTTTCGGTAAGAATCGACA
CAATACAACAGGATAATCGCCAACTTGCTTTTCACTGCTTTGAGCTTATTTCGCAACTGATTGAAGGTGAAACGCCATCC
CCATTACAGCGTTATCTTCCAGCCAGTCTACAAAAACGCTACCGATAA

Upstream 100 bases:

>100_bases
GCTTCGCTGATATTACAGGGCGAGTCTGACGTCACGTTACACTACTGGTCGCTACGCGCCTGCATGGTAGAATGAGGGTT
TTGAATTACGGATCCTCGTC

Downstream 100 bases:

>100_bases
TCCCGTCACTATCTTGCATCGCCCCTGTAAAACGGGGCGATTATTTTCATCCTGAACGTTAAATATTAATGATGAATAAT
CAAATCAGGCAATATTCAAA

Product: Sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERVLSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNY
GFAVFSHELETLCREAGVQLLISCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
PLVITDSVSPTATLVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQPEWIIHGNYHPSSGYEMFAALCA
RLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHLASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPS
PLQRYLPASLQKRYR

Sequences:

>Translated_335_residues
MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERVLSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNY
GFAVFSHELETLCREAGVQLLISCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
PLVITDSVSPTATLVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQPEWIIHGNYHPSSGYEMFAALCA
RLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHLASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPS
PLQRYLPASLQKRYR
>Mature_335_residues
MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERVLSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNY
GFAVFSHELETLCREAGVQLLISCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
PLVITDSVSPTATLVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQPEWIIHGNYHPSSGYEMFAALCA
RLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHLASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPS
PLQRYLPASLQKRYR

Specific function: Repressor for the scr operon. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=321, Percent_Identity=30.2180685358255, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=26.3322884012539, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789068, Length=279, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=25.5663430420712, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790369, Length=313, Percent_Identity=25.5591054313099, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=25.7731958762887, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787906, Length=298, Percent_Identity=24.496644295302, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789456, Length=342, Percent_Identity=27.1929824561404, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=25.5384615384615, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788474, Length=280, Percent_Identity=25.3571428571429, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790715, Length=161, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786540, Length=347, Percent_Identity=24.4956772334294, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37621; Mature: 37621

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERVLSIAQQQHYQPSIHAR
CCCCCCCHHHHHHHHHCCCHHHHHEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHH
SLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLISCTDENPSQETMVVNNMI
HHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
ARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTLPLVITDSVSPTATLVADIAR
HHHHCCHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHH
SHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQPEWIIHGNYHPSSGYEMFAALCA
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCHHHHHHHHHH
RLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHLASFDDHYLYDSLSVRIDTIQQDNR
HHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCHHHHHHEEEEEEHHCCCH
QLAFHCFELISQLIEGETPSPLQRYLPASLQKRYR
HHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCC
>Mature Secondary Structure
MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERVLSIAQQQHYQPSIHAR
CCCCCCCHHHHHHHHHCCCHHHHHEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHH
SLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLISCTDENPSQETMVVNNMI
HHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
ARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTLPLVITDSVSPTATLVADIAR
HHHHCCHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHH
SHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQPEWIIHGNYHPSSGYEMFAALCA
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCHHHHHHHHHH
RLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHLASFDDHYLYDSLSVRIDTIQQDNR
HHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCHHHHHHEEEEEEHHCCCH
QLAFHCFELISQLIEGETPSPLQRYLPASLQKRYR
HHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412665 [H]