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The map label for this gene is hydN

Identifier: 218701203

GI number: 218701203

Start: 3007969

End: 3008496

Strand: Reverse

Name: hydN

Synonym: ECIAI39_2899

Alternate gene names: 218701203

Gene position: 3008496-3007969 (Counterclockwise)

Preceding gene: 218701204

Following gene: 218701202

Centisome position: 58.62

GC content: 52.08

Gene sequence:

>528_bases
ATGAACCGTTTCATCATTGCTGACGCGAGTAAATGTATTGGTTGCCGTACCTGTGAAGTGGCGTGCGTGGTTTCTCATCA
GGAGAATCAGGACTGTGCATCGCTGACCCCGGAAACTTTTTTACCGCGAATCCATGTCATTAAAGGTGTGAACATTTCCA
CGGCAACAGTCTGCCGTCAGTGTGAAAATGCACCGTGCGCTAACGTCTGCCCGAATGGTGCTATCAGTCGTGATAAAGGG
TTTGTTCATGTGATGCAGGAACGTTGTATTGGTTGCAAAACCTGCGTTGTGGCTTGCCCGTATGGCGCGATGGAAGTGGT
GGTACGTCCGGTGATTCGCAACAGCGGCGCGGGGCTGAATGTACGGGCTGACAAAGCCGAAGCCAATAAATGCGACCTCT
GTAACCATCGTGAAGACGGCCCGGCGTGTATGGCGGCTTGCCCGACCCATGCGCTGATTTGTGTCGATCGTAATAAACTT
GAACAACTGAGCGCAGAAAAACGTCGCCGTACGGCGCTGATGTTCTAA

Upstream 100 bases:

>100_bases
CCTGCATTACAGAATTTAATTTCTTTAAATAACAGTAAATTAAAAACTGGCATGATTTGTGAATGTATCGGCGCATTAAC
TGTCATTGCTGGAGAATTTG

Downstream 100 bases:

>100_bases
AAATCTTACAGAACTGTTCTGGTATCAAAGGAATTCCCGGAAATATTCGTAGAATACGAAGCGGGCAGTATCGCCGACCT
GTGACTGCCGGATGCGACGC

Product: electron transport protein HydN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCENAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF

Sequences:

>Translated_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCENAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF
>Mature_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCENAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=99.4285714285714, Blast_Score=354, Evalue=2e-99,
Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=61.1111111111111, Blast_Score=193, Evalue=6e-51,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=48.2558139534884, Blast_Score=155, Evalue=1e-39,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=40.7185628742515, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=39.8843930635838, Blast_Score=131, Evalue=2e-32,
Organism=Escherichia coli, GI1789079, Length=190, Percent_Identity=38.9473684210526, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI87082114, Length=199, Percent_Identity=34.6733668341709, Blast_Score=104, Evalue=3e-24,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.2033898305085, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=32.5301204819277, Blast_Score=80, Evalue=5e-17,
Organism=Escherichia coli, GI226510944, Length=158, Percent_Identity=34.8101265822785, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI2367345, Length=154, Percent_Identity=33.7662337662338, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787963, Length=174, Percent_Identity=28.1609195402299, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19025; Mature: 19025

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
10.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
13.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CENAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLN
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHCCCCCCE
VRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF
EECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CENAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLN
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHCCCCCCE
VRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF
EECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]