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The map label for this gene is ascG

Identifier: 218701204

GI number: 218701204

Start: 3008645

End: 3009658

Strand: Reverse

Name: ascG

Synonym: ECIAI39_2900

Alternate gene names: 218701204

Gene position: 3009658-3008645 (Counterclockwise)

Preceding gene: 218701207

Following gene: 218701203

Centisome position: 58.64

GC content: 53.25

Gene sequence:

>1014_bases
ATGATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGTTTCAAAAGCGACCGTTTCCCGCGTGCTTTCAGGTAATGG
CTACGTCAGCCAGGAGACTAAAGATCGCGTGTTTCAGGCGGTAGAAGAGAGCGGTTACCGCCCAAACTTGCTGGCGCGCA
ATCTGTCGGCGAAGAGTACTCAGACGCTGGGGCTGGTAGTGACCAACACGCTTTACCACGGCATTTATTTTAGTGAACTA
CTGTTTCATGCCGCGCGAATGGCGGAAGAGAAAGGGCGACAGTTGCTATTGGCAGATGGTAAACACAGCGCCGAAGAAGA
GCGCCAGGCGATTCAGTATCTGCTGGATTTGCGCTGCGACGCCATTATGATTTACCCGCGTTTTTTAAGCGTGGATGAGA
TTGATGACATCATTGACGCACACAGTCAGCCGATAATGGTGCTTAATCGCCGCCTGCGCAAAAACACCAGCCATAGCGTC
TGGTGCGATCATAAACAGACCAGCTTTAACGCCGTGGCAGAGTTGATAAACGCCGGACATCAGGAGATTGCTTTCCTTAC
CGGCTCGATGGATTCCCCCACCAGCGTTGAACGTCTTGCCGGGTATAAAGACGCGCTGGCGCAGCATGGTATTGCGCTCA
ATGAAAAACTTATCGCTAACGGTAAATGGACGCCCGCCAGCGGGGCCGAAGGGGTAGAAACGTTGCTCGAACGTGGGGCT
AAATTTAGCGCGTTAGTTGCCAGTAACGACGATATGGCGATAGGTGCGATGAAAGCGTTGCACGAGCGCGGCGTAGCGGT
GCCAGAGCAGGTGTCAGTTATCGGATTCGATGATATCGCTATTGCCCCCTACACCGTTCCGGCGCTCTCCAGCGTAAAAA
TTCCGGTGACTGAGATGATTCAGGAAATTATTGGACGGCTGATTTTTATGCTCGATGGTGGGGATTTCTCACCGCCGAAA
ACCTTCAGCGGAAAACTGATCCGCCGCGGCTCCCTCATTGCTCTTTCGCAATAA

Upstream 100 bases:

>100_bases
TTTGTGAAACCGGTCACCTGAATATTTTTCCCGCCGATTGCGCAAGGACAATCGGCAAACGGTGCAGTACACTGCGTTGA
TTGCGTGTAAAGGGATGAAA

Downstream 100 bases:

>100_bases
ACATTGTCAACACATGTCATCGTCACTAGTGTCGATGACATCGTTTTTCCTGCATTACAGAATTTAATTTCTTTAAATAA
CAGTAAATTAAAAACTGGCA

Product: DNA-binding transcriptional repressor

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNTSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPK
TFSGKLIRRGSLIALSQ

Sequences:

>Translated_337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNTSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPK
TFSGKLIRRGSLIALSQ
>Mature_337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNTSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPK
TFSGKLIRRGSLIALSQ

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=98.2195845697329, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.6287425149701, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=33.6336336336336, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=30.5389221556886, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787948, Length=301, Percent_Identity=30.2325581395349, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=29.4117647058824, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790194, Length=318, Percent_Identity=27.9874213836478, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790715, Length=295, Percent_Identity=25.4237288135593, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787906, Length=317, Percent_Identity=29.0220820189274, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=24.9211356466877, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789456, Length=299, Percent_Identity=26.7558528428094, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=25.2459016393443, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36845; Mature: 36845

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
QTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
AIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNTSHSVWCDHKQTSFNAVAELINAGH
EEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
QEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCC
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMI
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCHHHHH
QEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIALSQ
HHHHHHHEEEECCCCCCCCCCCCCHHEECCCEEEECC
>Mature Secondary Structure
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
QTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
AIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNTSHSVWCDHKQTSFNAVAELINAGH
EEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
QEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCC
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYTVPALSSVKIPVTEMI
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCHHHHH
QEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIALSQ
HHHHHHHEEEECCCCCCCCCCCCCHHEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]