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The map label for this gene is hypF

Identifier: 218701202

GI number: 218701202

Start: 3005576

End: 3007888

Strand: Reverse

Name: hypF

Synonym: ECIAI39_2898

Alternate gene names: 218701202

Gene position: 3007888-3005576 (Counterclockwise)

Preceding gene: 218701203

Following gene: 218701199

Centisome position: 58.61

GC content: 58.24

Gene sequence:

>2313_bases
GTGACTGCCGGATGCGACGCGCTCGTGTCCGGCTGGGCGGGTTACGCAATTCATGATGGCGGGAATAACTCAATGGCAAA
AAACACATCTTGCGGTGTCCAACTGCGTATTCGTGGCAAAGTGCAGGGCGTCGGTTTTCGTCCGTTTGTCTGGCAACTGG
CACAGCAATTAAATCTTCACGGCGATGTTTGTAATGACGGCGATGGCGTAGAAGTCCGGCTGCTGGAAGCCCCGGAAACG
TTTCTTGTTCAATTGCATCAGCACTGCCCGCCACTGGCGCGTATTGATAGCGTCGAGCGTGAGCCGTTTATCTGGTCACA
ACTGCCCACTGAGTTCACCATCCGCCAGAGCGCGGGTGGCGTCATGAATACGCAAATTGTTCCCGATGCCGCGACTTGCC
CTGCTTGCCTTGCCGAAATGAATACCCCAGGCGAACGACGCTGGCGTTATCCGTTTATCAACTGTACCCACTGCGGCCCA
CGTTTCACCATTATTCGCGCGATGCCTTACGACCGCCCGTTTACCGTGATGGCGGCGTTTCCGCTGTGTCCGGCCTGTGA
CAAAGAATATCGCGACCCGCTCGATCGTCGCTTCCACGCCCAGCCGGTGGCCTGCCCGGAGTGTGGCCCACATCTTGAAT
GGGTAAGTCATGGTGAACATGCAGAACAAGAGGCGGCATTACAGGCAGCTATCGCACAGTTAAAAATGGGCAACATTGTC
GCCATCAAAGGGATTGGCGGATTTCATCTCGCCTGCGATGCGCGTAACAGTAACGCGGTGGCGACACTACGGGCACGCAA
ACATCGCCCGGCGAAACCGCTGGCGGTCATGTTGCCAGTGGCTGACGGTTTACCTGACGCTGCGCGCCAGTTGCTTACCA
CGCCCGCCGCGCCGATTGTTCTGGTGGATAAAAAATACGTTCCTGAGCTTTGTGATGATATCGCCCCTGGCCTTAACGAA
GTTGGGGTGATGTTGCCTGCGAACCCGCTCCAGCATTTGCTGTTACAGGAACTGCAATGCCCGCTGGTGATGACCTCCGG
CAACCTGAGCGGTAAACCACCAGCTATCAGCAACGAACAGGCGCTGGCGGATTTGCAGGGGATTGCCGACGGATTCTTGA
TACATAACCGCGACATCGTGCAGCGGATGGATGATTCGGTGGTGCGCGAAAACGGCGAAATGCTGCGCCGTTCGCGGGGG
TATGTGCCGGATGCGCTGGCTTTGCCTCCGGGCTTTAAAAATGTTCCGCCTGTGCTGTGTCTCGGTGCGGATCTGAAAAA
CACCTTCTGCCTGGTGCGCGGCGAACAAGCGGTGTTGAGTCAGCATTTGGGCGATTTAAGTGACGATGGCATCCAGATGC
AGTGGCGCGAAGCGTTACGCCTGATGCAAAACATCTACGATTTCACTCCGCAATACGTTGTGCATGACGCGCATCCGGGC
TATGTCTCCAGCCAGTGGGCGCGCGAAATGAATCTGCCGACGCAAACGGTACTGCATCATCATGCCCACGCGGCGGCGTG
TCTGGCAGAACATCACTGGCCGCTGGATGGCGGTGATGTCATTGCGTTGACGCTCGACGGTATCGGTATGGGGGAGAATG
GCGCTTTGTGGGGCGGCGAGTGTCTGCGGGTGAACTATCGCGAATGCCAGCATCTGGGCGGCTTGCCTGCGGTGGCGCTT
CCGGGTGGCGATTTTGCGGCGAAGCAGCCGTGGCGTAACCTGCTGGCGCAGTGCCTGCGCTTTGTGCCGGAGTGGCAGAA
TTACCCTGAAACGGCAAGTGTGCAACAGCAAAACTGGAGCGTGCTGGCGCGGGCCATTGAGCGTGGAATTAACGCGCCGC
TGGCGTCGTCGTGTGGGCGTTTGTTCGATGCTGTGGCGGCGGCACTGGGCTGTGCGCCAGCCATGTTAAGTTATGAAGGT
GAAGCGGCTTGCGCCCTCGAGGCGCTGGCGGCTTCGAGCCAGGGAGTGACGCATCCGGTGACGATACCGCTGGTGGACAA
TCAACTGGATCTCGCCACTTTCTGGCAGCAGTGGCTGAACTGGCAGGCACCGGTTAATCAACGCGCGTGGGCGTTTCATG
ATGCGCTGGCGCAGGGGTTTGCCGCGTTGATGCGTGAGCAGGCCACGATGCGCGGTATCACTACGCTGGTATTTAGCGGT
GGGGTTATTCATAACCGATTGCTGCGTACACGCCTGGCGCATTATCTCGCTGATTTCACATTGCTCTTTCCACAGAGTTT
ACCGGCGGGCGATGGCGGGTTGTCTCTGGGGCAGGGGGTTATTGTTGCGGCGCGCATCCTGCATGCTGCATAG

Upstream 100 bases:

>100_bases
GTACGGCGCTGATGTTCTAAAAATCTTACAGAACTGTTCTGGTATCAAAGGAATTCCCGGAAATATTCGTAGAATACGAA
GCGGGCAGTATCGCCGACCT

Downstream 100 bases:

>100_bases
TGGCGGATGTGGCGTAACGAAGCGTTAAAAGTAGCGCGGACAGTCCCCTCGCCCTTTGGGCGAGGGAGAAAACCATGCCT
ACATCGGCAACGTTTTCAAC

Product: carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MTAGCDALVSGWAGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEAPET
FLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPGERRWRYPFINCTHCGP
RFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIV
AIKGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNE
VGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRENGEMLRRSRG
YVPDALALPPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPG
YVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVAL
PGGDFAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPAMLSYEG
EAACALEALAASSQGVTHPVTIPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSG
GVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAARILHAA

Sequences:

>Translated_770_residues
MTAGCDALVSGWAGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEAPET
FLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPGERRWRYPFINCTHCGP
RFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIV
AIKGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNE
VGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRENGEMLRRSRG
YVPDALALPPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPG
YVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVAL
PGGDFAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPAMLSYEG
EAACALEALAASSQGVTHPVTIPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSG
GVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAARILHAA
>Mature_769_residues
TAGCDALVSGWAGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEAPETF
LVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPGERRWRYPFINCTHCGPR
FTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVA
IKGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEV
GVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRENGEMLRRSRGY
VPDALALPPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGY
VSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALP
GGDFAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPAMLSYEGE
AACALEALAASSQGVTHPVTIPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGG
VIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAARILHAA

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=743, Percent_Identity=97.0390309555855, Blast_Score=1385, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 83820; Mature: 83689

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGCDALVSGWAGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLH
CCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCC
GDVCNDGDGVEVRLLEAPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGG
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCC
VMNTQIVPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAF
EEECEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEC
PLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIV
CCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEE
AIKGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
EEECCCCEEEEEECCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEE
LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQ
EECCHHHHHHHHHHCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
ALADLQGIADGFLIHNRDIVQRMDDSVVRENGEMLRRSRGYVPDALALPPGFKNVPPVLC
HHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
LGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPG
ECCCCCCCEEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
YVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGENGALWGGE
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCC
CLRVNYRECQHLGGLPAVALPGGDFAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWS
EEEECHHHHHHCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VLARAIERGINAPLASSCGRLFDAVAAALGCAPAMLSYEGEAACALEALAASSQGVTHPV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCE
TIPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSG
EEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAARILHAA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TAGCDALVSGWAGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLH
CCCHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCC
GDVCNDGDGVEVRLLEAPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGG
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCC
VMNTQIVPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAF
EEECEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEEC
PLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIV
CCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEE
AIKGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
EEECCCCEEEEEECCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEE
LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQ
EECCHHHHHHHHHHCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
ALADLQGIADGFLIHNRDIVQRMDDSVVRENGEMLRRSRGYVPDALALPPGFKNVPPVLC
HHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
LGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPG
ECCCCCCCEEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
YVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGENGALWGGE
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCC
CLRVNYRECQHLGGLPAVALPGGDFAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWS
EEEECHHHHHHCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VLARAIERGINAPLASSCGRLFDAVAAALGCAPAMLSYEGEAACALEALAASSQGVTHPV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCE
TIPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSG
EEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAARILHAA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]