BacMap

Definition	Escherichia coli 55989, complete genome.
Accession	NC_011748
Length	5,154,862

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The map label for this gene is paaG

Identifier: 218694940

GI number: 218694940

Start: 1575729

End: 1576517

Strand: Direct

Name: paaG

Synonym: EC55989_1530

Alternate gene names: 218694940

Gene position: 1575729-1576517 (Clockwise)

Preceding gene: 218694939

Following gene: 218694941

Centisome position: 30.57

GC content: 55.13

Gene sequence:

>789_bases
ATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGACACTAACGCTCAACCGCCCGGAACGCCTGAACAGTTT
TAATGATGAGATGCACGCACAACTCGCAGAGAGCCTGAAACAGGCCGAGCGCGACGACACTATCCGTTGCCTGTTACTTA
CAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAATGTTGATCCCACAGGCCCCGCACCGGATTTA
GGGATGTCAGTGGAGCGTTTCTATAACCCGCTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAA
TGGCGTGGCGGCAGGGGCTGGCGCAACACTGGCACTGGGATGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCA
TGGCGTTTAGTAAGTTAGGCTTAGTACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCTGGGCGAGCGCGCGCC
ATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGATCTGGCAGGTTGTTGATGA
TGAAACGCTGGCAGATACCGCGCAACAGCTGGCGCGGCATCTGGCGACACAGCCGACATTTGGTCTTGGACTTATCAAGC
AAGCGATAAATAGCGCTGAAACCAATACGCTCGATATGCAACTGGATCTGGAACGCGACTATCAGCGACTTGCCGGACGG
AGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCCAAACGCTCACCGCAGTTCACGGGGAAATAG

Upstream 100 bases:

>100_bases
AGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCTGCTTTCTTACAAAAACGCTCG
CCTGACTTTAAAGGACGCTA

Downstream 100 bases:

>100_bases
CATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCTGGCATTGCTGAAGTTGCCGCCAGTC
ATGGACATCAGGTTTTACTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAESLKQAERDDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDMQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

Sequences:

>Translated_262_residues
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAESLKQAERDDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDMQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK
>Mature_262_residues
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAESLKQAERDDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDMQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=31.8548387096774, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=28.7937743190661, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=28.7937743190661, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=28.7937743190661, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=30.5970149253731, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=29.4372294372294, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=29.4372294372294, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI70995211, Length=208, Percent_Identity=29.8076923076923, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI20127408, Length=189, Percent_Identity=29.1005291005291, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4757968, Length=189, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI49355787, Length=189, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI221136753, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=30.939226519337, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI221307494, Length=168, Percent_Identity=31.547619047619, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=30.939226519337, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=28.8990825688073, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=25.6809338521401, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI150378541, Length=262, Percent_Identity=28.6259541984733, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI25453481, Length=189, Percent_Identity=28.042328042328, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538573, Length=189, Percent_Identity=28.042328042328, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=189, Percent_Identity=28.042328042328, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538581, Length=189, Percent_Identity=28.042328042328, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=28.1553398058252, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI116235487, Length=187, Percent_Identity=26.7379679144385, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=98.0916030534351, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=36.6412213740458, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=30.2681992337165, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=27.9693486590038, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=29.6, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=29.2307692307692, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=30, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=34, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.4347826086957, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=194, Percent_Identity=29.8969072164948, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=29.8701298701299, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=25, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=24.9027237354086, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=254, Percent_Identity=26.7716535433071, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=196, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=196, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=190, Percent_Identity=27.8947368421053, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=164, Percent_Identity=28.0487804878049, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=164, Percent_Identity=28.0487804878049, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=164, Percent_Identity=28.0487804878049, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=227, Percent_Identity=25.9911894273128, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=36.0153256704981, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=36.0153256704981, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=31.3432835820896, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=25.4752851711027, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=30.0448430493274, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24654903, Length=196, Percent_Identity=27.0408163265306, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=28.1746031746032, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=28.2926829268293, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28423; Mature: 28423

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAESLKQAERDDTIRCLLLTGAGRGF
CHHHHHHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
CDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
CEEEEEECCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHCHHHHHHCCE
IWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDMQLDLERDYQRLAGR
EEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCC
SADYREGVSAFLAKRSPQFTGK
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAESLKQAERDDTIRCLLLTGAGRGF
CHHHHHHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
CDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
CEEEEEECCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHCHHHHHHCCE
IWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDMQLDLERDYQRLAGR
EEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCC
SADYREGVSAFLAKRSPQFTGK
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]