BacMap

Definition	Escherichia coli 55989, complete genome.
Accession	NC_011748
Length	5,154,862

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The map label for this gene is paaF

Identifier: 218694939

GI number: 218694939

Start: 1574962

End: 1575729

Strand: Direct

Name: paaF

Synonym: EC55989_1529

Alternate gene names: 218694939

Gene position: 1574962-1575729 (Clockwise)

Preceding gene: 218694938

Following gene: 218694940

Centisome position: 30.55

GC content: 54.17

Gene sequence:

>768_bases
ATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAA
TAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCG
GTAATGCACGCTTTTTTGCCGCTGGGGCCGATCTCAACGAAATGGCAGAAAAAGATCTCGCGGCCACCTTAAACGATACA
CGTCCGCAGCTATGGGCGCGATTGCAGGCCTTTAATAAACCACTTATCGCAGCCGTCAATGGTTACGCGCTTGGGGCGGG
TTGCGAACTGGCATTGTTGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCA
TCATACCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGA
GAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGC
CTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGG
AAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGC
ATCTCTGCTTTCTTACAAAAACGCTCGCCTGACTTTAAAGGACGCTAA

Upstream 100 bases:

>100_bases
GTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGAC
TTTGACGCGAAGGGGATGGC

Downstream 100 bases:

>100_bases
TGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGACACTAACGCTCAACCGCCCGGAACGCCTGAACAGTTTT
AATGATGAGATGCACGCACA

Product: enoyl-CoA hydratase-isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIIPGAGGTQRLIRSVGKSLASKMVLSG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRSPDFKGR

Sequences:

>Translated_255_residues
MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDT
RPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIIPGAGGTQRLIRSVGKSLASKMVLSG
ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
ISAFLQKRSPDFKGR
>Mature_254_residues
SELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTR
PQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIIPGAGGTQRLIRSVGKSLASKMVLSGE
SITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEGI
SAFLQKRSPDFKGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=45.9349593495935, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=36.4341085271318, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI31542718, Length=239, Percent_Identity=31.3807531380753, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=232, Percent_Identity=32.7586206896552, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=29.9212598425197, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=27.734375, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=27.734375, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=27.734375, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI68989263, Length=253, Percent_Identity=31.6205533596838, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI20127408, Length=203, Percent_Identity=33.9901477832512, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI25453481, Length=259, Percent_Identity=26.6409266409266, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI51538573, Length=259, Percent_Identity=26.6409266409266, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI4757966, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI51538581, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=28.4090909090909, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=28.4090909090909, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221307494, Length=264, Percent_Identity=28.4090909090909, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=25.5813953488372, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=25.5813953488372, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI157694516, Length=223, Percent_Identity=29.1479820627803, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=223, Percent_Identity=29.1479820627803, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI116235487, Length=258, Percent_Identity=27.1317829457364, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI62530384, Length=229, Percent_Identity=27.5109170305677, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=26.4864864864865, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=26.4864864864865, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=163, Percent_Identity=31.9018404907975, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI157694520, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=99.2156862745098, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=36.6412213740458, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=31.9838056680162, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788682, Length=150, Percent_Identity=41.3333333333333, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=237, Percent_Identity=27.4261603375527, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=33.5195530726257, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=48, Blast_Score=247, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=48.1481481481481, Blast_Score=228, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=31.304347826087, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=217, Percent_Identity=29.9539170506912, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=31.1320754716981, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=29.1338582677165, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=32.2115384615385, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=29.6137339055794, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=238, Percent_Identity=28.9915966386555, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=25.8620689655172, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=33.5195530726257, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=267, Percent_Identity=25.8426966292135, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=267, Percent_Identity=25.8426966292135, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=28.2722513089005, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=46.3709677419355, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=46.3709677419355, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=34.3396226415094, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=29.6442687747036, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=30.1158301158301, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24654903, Length=180, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=24.8062015503876, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24650670, Length=257, Percent_Identity=26.4591439688716, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583165, Length=248, Percent_Identity=25.4032258064516, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921018, Length=235, Percent_Identity=26.8085106382979, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=33.9080459770115, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.9080459770115, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.9080459770115, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27206; Mature: 27074

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFA
CCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAG
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHHHHHCC
ENARFGLPEITLGIIPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCC
DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
ISAFLQKRSPDFKGR
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
SELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFA
CCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAG
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCEEECCCCHHHHHHHHHHCC
ENARFGLPEITLGIIPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPS
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCC
DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
ISAFLQKRSPDFKGR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]