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The map label for this gene is rutD

Identifier: 218694545

GI number: 218694545

Start: 1179398

End: 1180198

Strand: Reverse

Name: rutD

Synonym: EC55989_1120

Alternate gene names: 218694545

Gene position: 1180198-1179398 (Counterclockwise)

Preceding gene: 218694546

Following gene: 218694544

Centisome position: 22.89

GC content: 58.18

Gene sequence:

>801_bases
ATGAAACTTTCACTCTCACCTCCCCCTTATGCTGATGCGCCCGTAGTGGTGTTGATTTCGGGTCTTGGCGGTAGCGGCAG
TTACTGGTTACCGCAACTGGCGGTGCTGGAGCAGGAGTATCAGGTAGTCTGTTACGACCAGCGCGGCACCGGCAATAATC
CCGACACGCTGGCAGAAGATTACAGTATCGCCCAGATGGCAGCGGAACTGCATCAGGCGCTGGTAGCCGCAGGGATTGAG
CATTACGCGGTGGTCGGCCATGCGCTCGGTGCGCTGGTGGGAATGCAGCTGGCGCTGGATTATCCCGCGTCGGTAACTGT
GCTGATCAGCGTTAACGGCTGGCTACGAATAAACGCCCATACGCGCCGCTGTTTTCAGGTTCGCGAACGATTACTGTATA
GCGGCGGCGCGCAGGCATGGGTGGAAGCGCAGCCGTTGTTCCTCTATCCCGCCGACTGGATGGCGGCCCGCGCACCTCGC
CTTGAGGCAGAAGACGCGCTGGCACTGGCGCATTTTCAGGGCAAAAATAATTTACTGCGTCGACTTAACGCCCTCAAACG
CGCTGACTTTAGTCACCATGCGGATCGCATCCGCTGCCCGGTGCAAATCATCTGCGCCAGTGATGATCTGCTGGTGCCAT
CAGCATGTTCCAGTGAGCTTCATGCCGCCCTGCCCGATAGCCAGAAAATGGTGATGCGCTATGGCGGACACGCCTGCAAC
GTGACCGACCCCGAAACGTTTAATGCTCTTTTACTCAACGGGCTTGCCAGCCTGTTACATCACCGCGAAGCCGCCCTGTA
A

Upstream 100 bases:

>100_bases
GGTGATAAACCGGCGCGATTCTGCATTCAGTGCGGACTGGTAAAACCTGACGCGCTGGTGGAAATCGCCACAATTGCGCA
TATCGCCAAGTGAGGCCGCG

Downstream 100 bases:

>100_bases
GGAATTGCTATGAACGAAGCCGTTAGCCCAGGTGCGCTTAGCACCCTGTTCACCGATGCCCGCACTCACAACGGCTGGCG
GGAGACACCCGTCAGCGATG

Product: enzyme of the alternative pyrimidine degradation pathway

Products: NA

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIE
HYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
VTDPETFNALLLNGLASLLHHREAAL

Sequences:

>Translated_266_residues
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIE
HYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
VTDPETFNALLLNGLASLLHHREAAL
>Mature_266_residues
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIE
HYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
VTDPETFNALLLNGLASLLHHREAAL

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Escherichia coli, GI1787244, Length=266, Percent_Identity=99.2481203007519, Blast_Score=539, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 28943; Mature: 28943

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAED
CCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
YSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAH
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEECCEEEECHH
TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLR
HHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCCCHHHEEEEHCCCHHHHHH
RLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
HHHHHHHCCCHHCHHHCCCCEEEEECCCCEECCCCCCCHHHHCCCCHHHHHHHHCCCCCC
VTDPETFNALLLNGLASLLHHREAAL
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAED
CCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
YSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAH
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEECCEEEECHH
TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLR
HHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCCCHHHEEEEHCCCHHHHHH
RLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
HHHHHHHCCCHHCHHHCCCCEEEEECCCCEECCCCCCCHHHHCCCCHHHHHHHHCCCCCC
VTDPETFNALLLNGLASLLHHREAAL
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA