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The map label for this gene is rutE

Identifier: 218694544

GI number: 218694544

Start: 1178798

End: 1179388

Strand: Reverse

Name: rutE

Synonym: EC55989_1119

Alternate gene names: 218694544

Gene position: 1179388-1178798 (Counterclockwise)

Preceding gene: 218694545

Following gene: 218694543

Centisome position: 22.88

GC content: 55.67

Gene sequence:

>591_bases
ATGAACGAAGCCGTTAGCCCAGGTGCGCTTAGCACCCTGTTCACCGATGCCCGCACTCACAACGGCTGGCGGGAGACACC
CGTCAGCGATGAGACGTTACGGGAGCTTTATGCCCTGATGAAATGGGGGCCGACATCAGCTAACTGTTCTCCGGCACGGA
TCGTGTTTATCCGCACTGTAGAAGGAAAAGAACGTCTGCGCCCGGCGCTTTCCAGCGGCAATCTACAAAAAACCCTGACC
GCGCCCGTCACCGCTATCGTTGCCTGGGACAGTGAATTTTATGAACGGTTACCACTACTGTTTCCCCACGGTGATGCCCG
CAGTTGGTTTACCTCCAGCCCACAACTTGCCGAAGAAACAGCGTTTCGCAACAGCTCCATGCAGGCGGCCTATCTGATCG
TCGCTTGCCGTGCGCTGGGACTGGATACCGGCCCGATGTCGGGTTTTGACCGTCAATACGTTGACGACGCTTTTTTTGCA
GGCAGCACGCTGAAGAGCAATCTGCTGATTAATATCGGCTATGGCGATAGCAGCAAACTTTATGCGCGCCTGCCACGTCT
GTCCTTTGAAGAAGCCTGCGGGCTGTTGTAA

Upstream 100 bases:

>100_bases
CGCCTGCAACGTGACCGACCCCGAAACGTTTAATGCTCTTTTACTCAACGGGCTTGCCAGCCTGTTACATCACCGCGAAG
CCGCCCTGTAAGGAATTGCT

Downstream 100 bases:

>100_bases
GGAGCAAACTATGAACATAGTTGATCAACAAACCTTTCGCGATGCGATGTCCTGTATGGGCGCGGCGGTCAATATCATCA
CCACGGACGGTCCAGCCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGPTSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLT
APVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL

Sequences:

>Translated_196_residues
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGPTSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLT
APVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL
>Mature_196_residues
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGPTSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLT
APVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL

Specific function: May reduce toxic product malonic semialdehyde to 3- hydroxypropionic acid, which is excreted

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family. HadB/RutE subfamily

Homologues:

Organism=Escherichia coli, GI1787243, Length=196, Percent_Identity=97.4489795918367, Blast_Score=395, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTE_ECO24 (A7ZKB3)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462242.1
- ProteinModelPortal:   A7ZKB3
- SMR:   A7ZKB3
- STRING:   A7ZKB3
- EnsemblBacteria:   EBESCT00000022946
- GeneID:   5587315
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1126
- NMPDR:   fig|331111.3.peg.3609
- eggNOG:   COG0778
- GeneTree:   EBGT00050000010805
- HOGENOM:   HBG322534
- OMA:   MGPTSAN
- ProtClustDB:   PRK05365
- BioCyc:   ECOL331111:ECE24377A_1126-MONOMER
- HAMAP:   MF_01204
- InterPro:   IPR000415

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: =1.1.1.298

Molecular weight: Translated: 21607; Mature: 21607

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGPTSANCSPARIVFIRTV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEET
CCHHHHHHHHCCCCCEEEHHCCEEEEEEECHHHHHHCCEEECCCCCCCCCCCCCCHHHHH
AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFAGSTLKSNLLINIGYGDSSKL
HHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHH
YARLPRLSFEEACGLL
HHHCCCCCHHHHCCCC
>Mature Secondary Structure
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGPTSANCSPARIVFIRTV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEET
CCHHHHHHHHCCCCCEEEHHCCEEEEEEECHHHHHHCCEEECCCCCCCCCCCCCCHHHHH
AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFAGSTLKSNLLINIGYGDSSKL
HHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHH
YARLPRLSFEEACGLL
HHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA